Activin-actrii antagonists and uses for treating bone and other disorders

ABSTRACT

Provided herein are methods for the treatment of bone disorders that are associated with kidney disease wherein the methods comprise administration of Activin-ActRIIA inhibitors to a subject in need of the treatment. Also provided herein are methods and compositions for the treatment of low turnover bone disorders wherein the methods comprise administration of Activin-ActRIIA inhibitors to a subject in need of the treatment. Further provided herein are compositions for the treatment of bone disorders that are associated with kidney disease and compositions for the treatment of low turnover bone disorders and vascular calcification.

This application claims priority to U.S. Provisional Pat. ApplicationNo. 61/721,898, filed Nov. 2, 2012, and to U.S. Provisional Pat.Application No. 61/740,665, filed Dec. 21, 2012, the disclosures of eachof which are herein incorporated by reference in their entireties.

INTRODUCTION

Provided herein are methods for the treatment of bone disorders that areassociated with kidney disease, such as chronic kidney disease-mineraland bone disorder (“CKD-MBD”), wherein the methods compriseadministration of Activin-ActRII inhibitors to a subject in need of thetreatment. Also provided herein are methods and compositions for thetreatment of low turnover bone disorders wherein the methods compriseadministration of Activin-ActRII inhibitors to a subject in need of thetreatment. Also provided herein are compositions for the treatment ofbone disorders that are associated with kidney disease and compositionsfor the treatment of low turnover bone disorders and vascularcalcification.

BACKGROUND

Bone growth and mineralization are dependent on the activities of twocell types, osteoclasts and osteoblasts, although chondrocytes and cellsof the vasculature also participate in critical aspects of theseprocesses. Developmentally, bone formation occurs through twomechanisms, endochondral ossification and intramembranous ossification,with the former responsible for longitudinal bone formation and thelater responsible for the formation of topologically flat bones, such asthe bones of the skull. Endochondral ossification requires thesequential formation and degradation of cartilaginous structures in thegrowth plates that serve as templates for the formation of osteoblasts,osteoclasts, the vasculature and subsequent mineralization. Duringintramembranous ossification, bone is formed directly in the connectivetissues. Both processes require the infiltration of osteoblasts andsubsequent matrix deposition.

Chronic kidney disease is associated with a progressive deterioration inmineral homeostasis, with a disruption of normal serum and tissueconcentrations of phosphorus and calcium, and changes in circulatinghormones, such as parathyroid hormone, 25-hydroxyvitamin D,1,25-dihydroxyvitamin D, other vitamin D metabolites, fibroblast growthfactor-23, and growth hormone. See, Chronic Kidney Disease-Mineral andBone Disorder (CKD-MBD), Kidney Disease: Improving Global Outcomes(KDIGO) CKD-MBD Work Group, In: Kidney Int Suppl. (2009) 76 (Suppl113):S1-130, page S3. The mineral and hormone homeostasis that isdisrupted in chronic kidney disease is critical for initial boneformation during growth (bone modeling) and bone structure and functionduring adulthood (bone remodeling). As a result, bone abnormalities arefound in patients with chronic kidney disease. In addition, similarlydue to the disruption in mineral and endocrine functions, extraskeletalcalcification may be found in patients with chronic kidney disease.These syndromes are termed chronic kidney disease-related mineral andbone disorders (“CDK-MBD”).

Bone undergoes continuous turnover. Bone turnover is the process ofresorption followed by replacement of bone. Osteoblasts and osteoclastsare the cells necessary for bone turnover. Low turnover and adynamicbone diseases are characterized by reduced or absent resorption andreplacement of bone. CKD-MBD can be characterized by low turnover oradynamic bone. (Chronic Kidney Disease-Mineral and Bone Disorder(CKD-MBD), Kidney Disease: Improving Global Outcomes (KDIGO) CKD-MBDWork Group, In: Kidney Int Suppl. (2009) 76 (Suppl 113):S1-130, pageS34).

Increased calcium levels in the vasculature can lead to vascularcalcification, a condition characterized by increased vessel stiffening.Patients with vascular calcification have an increased risk ofmyocardial infarction, and vascular calcification is particularlyprevalent in patients suffering from kidney disease, e.g., CKD-MBD. See,e.g., Shanahan et al., 2011, Circ. Res. 109:697-711.

Two related type II receptors, ActRIIA and ActRIIB, have been identifiedas the type II receptors for activins (Mathews and Vale, 1991, Cell65:973-982; Attisano et al., 1992, Cell 68: 97-108). Besides activins,ActRIIA and ActRIIB can biochemically interact with several otherTGF-beta family proteins, including BMP7, Nodal, GDF8, and GDF11(Yamashita et al., 1995, J. Cell Biol. 130:217-226; Lee and McPherron,2001, Proc. Natl. Acad. Sci. 98:9306-9311; Yeo and Whitman, 2001, Mol.Cell 7: 949-957; Oh et al., 2002, Genes Dev. 16:2749-54). ALK4 is theprimary type I receptor for activins, particularly for activin A, andALK-7 may serve as a receptor for activins as well, particularly foractivin B.

SUMMARY

In certain embodiments, provided herein are methods for treating anadynamic bone disorder in a subject, wherein the method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto a subject in need of treatment of the adynamic bone disorder. Furtherprovided herein are methods for treating an adynamic bone disorder formof CKD-MBD in a subject, wherein the method comprises administering atherapeutically effective amount of an ActRII inhibitor to a subject inneed of treatment of the adynamic bone disorder form of CKD-MBD.

In certain more specific embodiments, the adynamic bone disorder ischaracterized by absence of tetracycline incorporation into mineralizedbone.

In certain embodiments, provided herein are methods for treating a lowbone turnover form of CKD-MBD in a subject, wherein the method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto a subject in need of treatment of the low bone turnover form ofCKD-MBD. In a more specific embodiment, the low bone turnover form ofCKD-MBD is osteomalacia.

In certain embodiments, provided herein are methods for treating a bonedisorder characterized by hyperphosphatemia in a subject, wherein themethod comprises administering a therapeutically effective amount of anActRII inhibitor to a subject in need of treatment of the bone disordercharacterized by hyperphosphatemia.

In certain embodiments, provided herein are methods for treatingatherosclerotic calcification in a subject, wherein the method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto a subject in need of treatment of atherosclerotic calcification.

In certain embodiments, provided herein are methods for treating a renaldisease in a subject, wherein the method comprises administering atherapeutically effective amount of an ActRII inhibitor to a subject inneed of treatment of the renal disease. In a more specific embodiment,the renal disease is renal fibrosis.

In a specific embodiment, provided herein is a method for treatingextraskeletal calcification in a subject, wherein said method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto the subject. In another specific embodiment, provided herein is amethod for preventing extraskeletal calcification in a subject, whereinsaid method comprises administering a therapeutically effective amountof an ActRII inhibitor to the subject. In specific embodiments, theextraskeletal calcification treated or prevented in a subject by themethods described herein is vascular calcification, i.e., theaccumulation of calcium salts in the vasculature of the subject, e.g.,calcification of arteries of the subject.

In certain embodiments, the ActRII inhibitor that can be used with themethods provided herein is a polypeptide comprising an amino acidsequence selected from the group consisting of: 90% identical to SEQ IDNO:2; 95% identical to SEQ ID NO:2; 98% identical to SEQ ID NO:2; SEQ IDNO:2; 90% identical to SEQ ID NO:3; 95% identical to SEQ ID NO:3; 98%identical to SEQ ID NO:3; SEQ ID NO:3; 90% identical to SEQ ID NO:6; 95%identical to SEQ ID NO:6; 98% identical to SEQ ID NO:6; SEQ ID NO:6; 90%identical to SEQ ID NO:7; 95% identical to SEQ ID NO:7; 98% identical toSEQ ID NO:7; SEQ ID NO:7; 90% identical to SEQ ID NO: 12; 95% identicalto SEQ ID NO: 12; 98% identical to SEQ ID NO: 12; SEQ ID NO: 12; 90%identical to SEQ ID NO:17; 95% identical to SEQ ID NO:17; 98% identicalto SEQ ID NO:17; SEQ ID NO:17; 90% identical to SEQ ID NO:20; 95%identical to SEQ ID NO:20; 98% identical to SEQ ID NO:20; SEQ ID NO:20;90% identical to SEQ ID NO:21; 95% identical to SEQ ID NO:21; 98%identical to SEQ ID NO:21; and SEQ ID NO:21. In a more specificembodiment, the ActRII inhibitor is a polypeptide comprising the aminoacid sequence of SEQ ID NO:7. In a more specific embodiment, the ActRIIinhibitor is administered parentally.

In a specific embodiment, the ActRII inhibitor that can be used with themethods provided herein is an ActRIIA inhibitor, wherein the ActRIIAinhibitor comprises or consists of a polypeptide selected from the groupconsisting of: a. a polypeptide at least 90% identical to SEQ ID NO:2;b. a polypeptide at least 95% identical to SEQ ID NO:2; c. a polypeptideat least 98% identical to SEQ ID NO:2; d. SEQ ID NO:2; e. a polypeptideat least 90% identical to SEQ ID NO:3; f. a polypeptide at least 95%identical to SEQ ID NO:3; g. a polypeptide at least 98% identical to SEQID NO:3; h. SEQ ID NO:3; i. a polypeptide at least 90% identical to SEQID NO:6; j. a polypeptide at least 95% identical to SEQ ID NO:6; k. apolypeptide at least 98% identical to SEQ ID NO:6; 1. SEQ ID NO:6; m. apolypeptide at least 90% identical to SEQ ID NO:7; n. a polypeptide atleast 95% identical to SEQ ID NO:7; o. a polypeptide at least 98%identical to SEQ ID NO:7; p. SEQ ID NO:7; q. a polypeptide at least 90%identical to SEQ ID NO: 12; r. a polypeptide at least 95% identical toSEQ ID NO: 12; s. a polypeptide at least 98% identical to SEQ ID NO: 12;and t. SEQ ID NO: 12. In a specific embodiment, the ActRIIA inhibitor isa polypeptide comprising or consisting of the amino acid sequence of SEQID NO:7.

In another specific embodiment, the ActRII inhibitor that can be usedwith the methods provided herein is an ActRIIB inhibitor, wherein theActRIIB inhibitor comprises or consists of a polypeptide selected fromthe group consisting of: a. a polypeptide at least 90% identical to SEQID NO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, or 43; b. apolypeptide at least 95% identical to SEQ ID NO:17, 18, 23, 26, 27, 29,30, 31, 32, 33, 36, 37, 42, or 43; c. a polypeptide at least 98%identical to SEQ ID NO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37,42, or 43; d. SEQ ID NO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37,42, or 43; e. a polypeptide 90% identical to SEQ ID NO:20, 21, 24, 25,34, 35, 38, 39, 40, 41, 44, 46, or 47; f. a polypeptide 95% identical toSEQ ID NO:20, 21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46, or 47; g. apolypeptide 98% identical to SEQ ID NO:20, 21, 24, 25, 34, 35, 38, 39,40, 41, 44, 46, or 47; and h. SEQ ID NO:20, 21, 24, 25, 34, 35, 38, 39,40, 41, 44, 46, or 47. In a specific embodiment, the ActRIIB inhibitoris a polypeptide comprising or consisting of SEQ ID NO:23. In anotherspecific embodiment, the ActRIIB inhibitor is a polypeptide comprisingor consisting of SEQ ID NO:25.

In another specific embodiment, an ActRIIA inhibitor and an ActRIIBinhibitor can be used in the methods provided herein (e.g., acomposition comprising an ActRIIA inhibitor and an ActRIIB inhibitor canbe used; or an ActRIIA inhibitor and an ActRIIB inhibitor can both beadministered, separately, to a subject being treated in accordance withthe methods described herein), wherein the ActRIIA inhibitor comprisesor consists of a polypeptide selected from the group consisting of: a. apolypeptide at least 90% identical to SEQ ID NO:2; b. a polypeptide atleast 95% identical to SEQ ID NO:2; c. a polypeptide at least 98%identical to SEQ ID NO:2; d. SEQ ID NO:2; e. a polypeptide at least 90%identical to SEQ ID NO:3; f. a polypeptide at least 95% identical to SEQID NO:3; g. a polypeptide at least 98% identical to SEQ ID NO:3; h. SEQID NO:3; i. a polypeptide at least 90% identical to SEQ ID NO:6; j. apolypeptide at least 95% identical to SEQ ID NO:6; k. a polypeptide atleast 98% identical to SEQ ID NO:6; 1. SEQ ID NO:6; m. a polypeptide atleast 90% identical to SEQ ID NO:7; n. a polypeptide at least 95%identical to SEQ ID NO:7; o. a polypeptide at least 98% identical to SEQID NO:7; p. SEQ ID NO:7; q. a polypeptide at least 90% identical to SEQID NO: 12; r. a polypeptide at least 95% identical to SEQ ID NO: 12; s.a polypeptide at least 98% identical to SEQ ID NO: 12; and t. SEQ ID NO:12; and wherein the ActRIIB inhibitor comprises or consists of apolypeptide selected from the group consisting of: a. a polypeptide atleast 90% identical to SEQ ID NO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33,36, 37, 42, or 43; b. a polypeptide at least 95% identical to SEQ IDNO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, or 43; c. apolypeptide at least 98% identical to SEQ ID NO:17, 18, 23, 26, 27, 29,30, 31, 32, 33, 36, 37, 42, or 43; d. SEQ ID NO: 17, 18, 23, 26, 27, 29,30, 31, 32, 33, 36, 37, 42, or 43; e. a polypeptide 90% identical to SEQID NO:20, 21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46, or 47; f. apolypeptide 95% identical to SEQ ID NO:20, 21, 24, 25, 34, 35, 38, 39,40, 41, 44, 46, or 47; g. a polypeptide 98% identical to SEQ ID NO:20,21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46, or 47; and h. SEQ ID NO:20,21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46, or 47. In a specificembodiment, the ActRIIA inhibitor is a polypeptide comprising orconsisting of SEQ ID NO:7 and the ActRIIB inhibitor is a polypeptidecomprising or consisting of SEQ ID NO:23. In another specificembodiment, the ActRIIA inhibitor is a polypeptide comprising orconsisting of SEQ ID NO:7 and the ActRIIB inhibitor is a polypeptidecomprising or consisting of SEQ ID NO:25.

In certain embodiments, the subject to be treated with the methodsprovided herein is less than 18 years old. In certain embodiments, thesubject to be treated with the methods provided herein has end stagerenal disease. In certain embodiments, the subject to be treated withthe methods provided herein undergoes dialysis. In certain embodiments,provided herein is a method to increase the height of the subject.

In certain embodiments, provided herein are methods for treating orpreventing hyperphosphatemia, secondary hyperparathyroidism (due toincrease in phosphorus), extraskeletal calcification, e.g., vascularcalcification, and adynamic bone disorder in a subject, wherein themethod comprises administering a therapeutically effective amount of anActRII inhibitor to a subject in need of treatment of hyperphosphatemia,secondary hyperparathyroidism (due to increase in phosphorus),extraskeletal calcification, e.g., vascular calcification, and adynamicbone.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 : Mouse body weight following partial nephrectomy.

FIG. 2 : Changes in BMD by DEXA Scan following partial nephrectomy inmice.

FIG. 3 : The murine counterpart of SEQ ID NO 7 (“mActRIIA-Fc”)hematocrit changes following partial nephrectomy in mice.

FIG. 4 : MicroCT 3D image of representative bones following partialnephrectomy in mice.

FIG. 5 : mActRIIA-Fe treatment Increases Hematocrit.

FIG. 6 : mActRIIA-Fe increases Bone Mineral Density.

FIG. 7 : Representative microCT Scans of Femurs.

FIG. 8 : mActRIIA-Fe increases Cortical Thickness of the FemurMid-Shaft.

FIG. 9 : mActRIIA-Fe Increases Trabecular Bone Volume.

FIG. 10 : mActRIIA-Fc Increases Trabecular Thickness in the DistalFemur.

FIG. 11 : mActRIIA-Fe causes a reduction in the levels of aortic calciumin a CKD mouse model.

DETAILED DESCRIPTION 5.1 Overview

Provided herein, in one aspect, is a method for the treatment of ChronicKidney Disease-Mineral and Bone Disorders (CKD-MBD) wherein the methodcomprises administering an inhibitor of ActRII to a patient in need oftreatment. The inhibitor of ActRII can be an inhibitor of ActRIIA and /or ActRIIB.

CKD-MBD can be diagnosed as a systemic disorder of mineral and bonemetabolism due to chronic kidney disease and manifested by either one ora combination of (1) abnormalities of calcium; phosphorus; calcium xphosphorus product; alkaline phosphatases (total or bone specific);bicarbonate; parathyroid hormone (“PTH”); 1-84 PTH, 1-84-PTH/7-84 PTHratio; osteocalcin; osteoprotegrin; tartrate-resistant acid phosphataseisoform 5b (“TRAP-5b”); pyridinoline and deoxypyridinoline; procollagentype 1 amino-terminal extension peptides; C-terminal crosslinks;C-terminal crosslinks of collagen; fibroblast growth factor 23(“FGF23”); Fetulin-A; or vitamin D metabolism; (2) abnormalities of boneturnover, mineralization, volume, linear growth, or strength; and (3)vascular or other soft tissue calcification. See Nickolas, 2008, KidneyInternational 74:721-731; and Moe et al., 2006, Kidney International69:1945-1953. Guidelines for the diagnosis of CKD-MBD can be found,e.g., in KDIGO clinical practice guidelines for the prevention,diagnosis, evaluation, and treatment of Chronic Kidney Disease-Mineraland Bone Disorder (CKD-MBD), Kidney Disease: Improving Global Outcomes(KDIGO) CKD-MBD Work Group, In: Kidney Int Suppl. (2009) 76 (Suppl113):S1-130.

In certain embodiments, provided herein are methods for the treatment oflow bone turnover forms of CKD-MBD wherein the method comprisesadministering an inhibitor of ActRII to a patient in need of treatment.In certain embodiments, provided herein are methods for the treatment ofCKD-MBD characterized by hyperphosphatemia and / or hypercalcemia. Incertain embodiments, provided herein are methods for the treatment ofCKD-MBD characterized by extraskeletal calcification, such as, but notlimited to atherosclerotic calcification.

In certain embodiments, provided herein are methods for the treatment ofCKD-MBD, wherein the chronic kidney disease has reached stage 3, stage4, stage 5, or stage 5D. In a specific embodiment, the kidney disease isend stage kidney disease. In certain embodiments, provided herein aremethods for the treatment of CKD-MBD characterized by a glomerularfiltration rate of less than 60 ml/min/1.73 m² in adults or less than 89ml/min/1.73 m² in pediatric patients. See, Moe et al., 2006, KidneyInternational 69:1945-1953. In certain embodiments, provided herein aremethods for the treatment in adults of CKD-MBD characterized by aglomerular filtration rate of less than 50 ml/min/1.73 m², 40ml/min/1.73 m², 30 ml/min/1.73 m², 20 ml/min/1.73 m², or less than 10ml/min/1.73 m². In certain embodiments, provided herein are methods forthe treatment in pediatric patients of CKD-MBD characterized by aglomerular filtration rate of less than 80 ml/min/1.73 m², 70ml/min/1.73 m², 60 ml/min/1.73 m², 50 ml/min/1.73 m², 40 ml/min/1.73 m²,30 ml/min/1.73 m², 20 ml/min/1.73 m², or less than 10 ml/min/1.73 m².

Without being bound by theory, a glomerular filtration rate of less than60 ml/min/1.73 m² in adult patients and less than 89 ml/min/1.73 m² inpediatric patients results in detectable abnormalities in calciumlevels, phosphorus levels, PTH levels, and vitamin D metabolism; andabnormal levels of these markers result in bone disease.

In certain embodiments, provided herein are methods for the treatment ofa bone pathology associated with chronic kidney disease, i.e., CKD-MBD.See Moe et al., 2006, Kidney International 69:1945-1953. In certainembodiments, the CKD-MBD is low-turnover CKD-MBD. Low-turnover CKD-MBDcan be diagnosed by the histological features set forth in Table 1below. See National Kidney Foundation, Kidney Disease Outcomes QualityInitiative Guidelines at the website of the National Kidney Foundation.

TABLE 1 Histological Features of Low-Turnover CKD-MBD Feature AdynamicOsteomalacia Bone Formation Trabecular bone volume Normal, low VariableLow, normal or high Osteoid volume Normal, low High-very high Osteoidseam thickness Normal, low High-very high Number of osteoblasts Low LowBone formation rate Low-very low Low-very low Mineralization lag timeNormal Prolonged Bone Resorption Eroded bone perimeter Normal, lowVariable Often low, may be high Number of osteoclasts Low Low, may benormal or high Marrow fibrosis Absent Absent

In a specific embodiment, provided herein is a method for treatingextraskeletal calcification in a subject, wherein said method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto the subject. In another specific embodiment, provided herein is amethod for preventing extraskeletal calcification in a subject, whereinsaid method comprises administering a therapeutically effective amountof an ActRII inhibitor to the subject. In specific embodiments, theextraskeletal calcification treated or prevented in a subject by themethods described herein is vascular calcification, i.e., theaccumulation of calcium salts in the vasculature of the subject, e.g.,calcification of arteries of the subject.

In certain embodiments, the methods of of treatment or prevention ofextraskeletal calcification, e.g., vascular calcification, providedherein are performed on a subject that is at risk of suffering fromextraskeletal calcification, e.g., vascular calcification (i.e., the atrisk subject is administered an ActRII inhibitor in accordance with themethods described herein). In a specific embodiment, the subject at riskof suffering from extraskeletal calcification, e.g., vascularcalcification, has hypercholesterolemia. In another specific embodiment,the subject at risk of suffering from extraskeletal calcification, e.g.,vascular calcification, has hypertension. In another specificembodiment, the subject at risk of suffering from extraskeletalcalcification, e.g., vascular calcification, has diabetes. In anotherspecific embodiment, the subject at risk of suffering from extraskeletalcalcification, e.g., vascular calcification, has renal disease (e.g.,end-stage renal disease). In another specific embodiment, the subject atrisk of suffering from extraskeletal calcification, e.g., vascularcalcification, has chronic kidney disease. In another specificembodiment, the subject at risk of suffering from cxtraskclctalcalcification, e.g., vascular calcification, has increased oxidativestress, e.g., an imbalance between oxidant production and antioxidantactivity in the vasculature. In another specific embodiment, the subjectat risk of suffering from extraskeletal calcification, e.g., vascularcalcification, has a calcification inhibitor deficiency (e.g., adeficiency in one or more of fetuin-A, matrix gla protein (MGP), andosteoprotegerin (OPG)).

In certain embodiments, the subjects suffering from vascularcalcification treated in accordance with the methods described hereinhave Media calcification (also known as Mönckeberg’s sclerosis or mediacalcinosis). Media calcification is characterized by diffuse mineraldeposits within the arterial tunica media. In a specific embodiment, thesubjects suffering from media calcification are elderly. In a specificembodiment, the subjects suffering from media calcification have adisorder that causes the Media calcification, e.g., diabetes, renaldisease (e.g., CKD).

In certain embodiments, the subjects suffering from vascularcalcification treated in accordance with the methods described hereinhave Intima calcification. Intima calcification is associated withatherosclerosis and progresses as atherosclerotic plaques progress.

In certain embodiments, a subject suffering from, or at risk ofsuffering from, a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification, has increased levels of FGF23, a hormoneproduced by osteocytes in response to decreased mechanical loading,decreases in bone formation and to excess phosphorus in the exchangablepool (see, e.g., Hruska and Mathew, 2011, Advances in Chronic KidneyDisease 18(2):98-104), relative to FGF23 levels in subjects that are notsuffering from, or not at risk of suffering from, a form of CKD-MBDand/or extraskeletal calcification, e.g., vascular calcification. Levelsof FGF23 can be detected using methods known in the art, e.g., ELISA,using samples from the subjects, e.g, blood, serum. In a specificembodiment, the level of FGF23 (e.g., the level detectable in the serum)in a subject suffering from, or at risk of suffering from, a form ofCKD-MBD and/or extraskeletal calcification, e.g., vascularcalcification, is about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%,or greater than 50%, greater than the level of FGF23 (e.g., the leveldetectable in the serum) in a subject not suffering from, or not at riskof suffering from, a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification. In another specific embodiment, the levelof FGF23 (e.g., the level detectable in the serum) in a subjectsuffering from, or at risk of suffering from, a form of CKD-MBD and/orextraskeletal calcification, e.g., vascular calcification, is about5-10%, 10-20%, 20-30%, 30-40%, 40-50%, 50-60%, 50-75%, or 75-100%,greater than the level of FGF23 (e.g., the level detectable in theserum) in a subject not suffering from, or not at risk of sufferingfrom, a form of CKD-MBD and/or extraskeletal calcification, e.g.,vascular calcification.

In certain embodiments, levels of FGF23 in a subject suffering from, orat risk of suffering from, a form of CKD-MBD and/or extraskeletalcalcification, e.g., vascular calcification, can be used to monitor theeffectiveness of a method described herein, e.g., a method of treating aform of CKD-MBD and/or a method of treating extraskeletal calcification(e.g., vascular calcification), wherein such methods compriseadministration of a therapeutically effective amount of an ActRIIinhibitor described herein. In a specific embodiment, a subject treatedin accordance with one or more of the methods described herein has adecreased level of FGF23 (e.g., as detected in the serum of the subject)as compared to the level of FGF23 detected in the subject prior to beingtreated with a method described herein. In another specific embodiment,the level of FGF23 (e.g., the level detectable in the serum) in asubject suffering from, or at risk of suffering from, a form of CKD-MBDand/or extraskeletal calcification, e.g., vascular calcification,treated with a method described herein is decreased by about 5%, 10%,15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, or greater than 50%, relative tothe level of FGF23 (e.g., the level detectable in the serum) detected inthe subject prior to treatment with a method described herein. Inanother specific embodiment, the level of FGF23 (e.g., the leveldetectable in the serum) in a subject suffering from, or at risk ofsuffering from, a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification, is decreased by about 5-10%, 10-20%,20-30%, 30-40%, 40-50%, 50-60%, 50-75%, or 75-100%, relative to thelevel of FGF23 (e.g., the level detectable in the serum) detected in thesubject prior to treatment with a method described herein.

In a specific embodiment, provided herein is a method of treating a formof CKD-MBD and/or extraskeletal calcification, e.g., vascularcalcification, comprising: (i) administering an ActRII inhibitor to anindividual having a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification; (ii) determining an amount of FGF23 in atissue sample (e.g., serum) of said individual after the adminstrationof the ActRII inhibitor; and (iii) if the amount of FGF23 in said tissuesample is decreased by no more than about 5%, 10%, 15%, 20%, or 25%, orby about 5-10%, 10-20%, 20-30%, as compared to the amount of FGF23determined in a sample of the same tissue type from said individual(e.g., a different sample of serum from the same individual) prior toadministration of the ActRII inhibitor, repeating the administration ofthe ActRII inhibitor. In certain embodiments, if the amount of FGF23 isnot decreased following administration of the ActRII inhibitor, the doseof the ActRII inhibitor administered can be increased. In certainembodiments, if the amount of FGF23 is not decreased followingadministration of the ActRII inhibitor, the frequency of administrationof the ActRII inhibitor administered can be increased.

In certain embodiments, a subject suffering from, or at risk ofsuffering from, a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification, has increased levels of sclerostin, aprotein increased in subjects suffering from, or at risk of sufferingfrom, CKD-MBD (see, e.g., Graciolli et al., 2010, J Am Soc Nephrol 21:774A), relative to sclerostin levels in subjects that are not sufferingfrom, or not at risk of suffering from, a form of CKD-MBD and/orextraskeletal calcification, e.g., vascular calcification. Levels ofsclerostin can be detected using methods known in the art, e.g., ELISA,using samples from the subjects, e.g, blood, serum. In a specificembodiment, the level of sclerostin (e.g., the level detectable in theserum) in a subject suffering from, or at risk of suffering from, a formof CKD-MBD and/or extraskeletal calcification, e.g., vascularcalcification, is about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%,or greater than 50%, greater than the level of sclerostin (e.g., thelevel detectable in the serum) in a subject not suffering from, or notat risk of suffering from, a form of CKD-MBD and/or extraskeletalcalcification, e.g., vascular calcification. In another specificembodiment, the level of sclerostin (e.g., the level detectable in theserum) in a subject suffering from, or at risk of suffering from, a formof CKD-MBD and/or extraskeletal calcification, e.g., vascularcalcification, is about 5-10%, 10-20%, 20-30%, 30-40%, 40-50%, 50-60%,50-75%, or 75-100%, greater than the level of sclerostin (e.g., thelevel detectable in the serum) in a subject not suffering from, or notat risk of suffering from, a form of CKD-MBD and/or extraskeletalcalcification, e.g., vascular calcification.

In certain embodiments, levels of sclerostin in a subject sufferingfrom, or at risk of suffering from, a form of CKD-MBD and/orextraskeletal calcification, e.g., vascular calcification, can be usedto monitor the effectiveness of a method described herein, e.g., amethod of treating a form of CKD-MBD and/or a method of treatingextraskeletal calcification (e.g., vascular calcification), wherein suchmethods comprise administration of a therapeutically effective amount ofan ActRII inhibitor described herein. In a specific embodiment, asubject treated in accordance with one or more of the methods describedherein has a decreased level of sclerostin (e.g., as detected in theserum of the subject) as compared to the level of sclerostin detected inthe subject prior to being treated with a method described herein. Inanother specific embodiment, the level of sclerostin (e.g., the leveldetectable in the serum) in a subject suffering from, or at risk ofsuffering from, a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification, treated with a method described herein isdecreased by about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, orgreater than 50%, relative to the level of sclerostin (e.g., the leveldetectable in the serum) detected in the subject prior to treatment witha method described herein. In another specific embodiment, the level ofsclerostin (e.g., the level detectable in the serum) in a subjectsuffering from, or at risk of suffering from, a form of CKD-MBD and/orextraskeletal calcification, e.g., vascular calcification, is decreasedby about 5-10%, 10-20%, 20-30%, 30-40%, 40-50%, 50-60%, 50-75%, or75-100%, relative to the level of sclerostin (e.g., the level detectablein the serum) detected in the subject prior to treatment with a methoddescribed herein.

In a specific embodiment, provided herein is a method of treating a formof CKD-MBD and/or extraskeletal calcification, e.g., vascularcalcification, comprising: (i) administering an ActRII inhibitor to anindividual having a form of CKD-MBD and/or extraskeletal calcification,e.g., vascular calcification; (ii) determining an amount of sclerostinin a tissue sample (e.g., serum) of said individual after theadminstration of the ActRII inhibitor; and (iii) if the amount ofsclerostin in said tissue sample is decreased by no more than about 5%,10%, 15%, 20%, or 25%, or by about 5-10%, 10-20%, 20-30%, as compared tothe amount of sclerostin determined in a sample of the same tissue fromsaid individual (e.g., a different sample of serum from the sameindividual) prior to administration of the ActRII inhibitor, repeatingthe administration of the ActRII inhibitor. In certain embodiments, ifthe amount of sclerostin is not decreased following administration ofthe ActRII inhibitor, the dose of the ActRII inhibitor administered canbe increased. In certain embodiments, if the amount of sclerostin is notdecreased following administration of the ActRII inhibitor, thefrequency of administration of the ActRII inhibitor administered can beincreased.

In certain embodiments, the subject suffering from vascularcalcification treated in accordance with the methods described herein isless than 18 years old. In a specific embodiment, the subject sufferingfrom vascular calcification treated in accordance with the methodsdescribed herein is less than 13 years old. In another specificembodiment, the subject suffering from vascular calcification treated inaccordance with the methods described herein is less than 12, less than11, less than 10, less than 9, less than 8, less than 7, less than 6, orless than 5 years old. In another specific embodiment, the subjectsuffering from vascular calcification treated in accordance with themethods described herein is 1-3 years old, 3-5 years old, 5-7 years old,7-9 years old, 9-11 years old, 11-13 years old, 13-15 years old, 15-20years old, 20-25 years old, 25-30 years old, or greater than 30 yearsold. In another specific embodiment, the subject suffering from vascularcalcification treated in accordance with the methods described herein is30-35 years old, 35-40 years old, 40-45 years old, 45-50 years old,50-55 years old, 55-60 years old, or greater than 60 years old. Inanother specific embodiment, the subject suffering from vascularcalcification treated in accordance with the methods described herein is60-65 years old, 65-70 years old, 70-75 years old, 75-80 years old, orgreater than 80 years old.

In certain embodiments, the subject suffering from vascularcalcification treated in accordance with the methods described hereinhas end stage renal disease. In certain embodiments, the subjectsuffering from vascular calcification treated in accordance with themethods described herein undergoes dialysis.

In certain embodiments, the effectiveness of treatment or prevention ofextraskeletal calcification, e.g., vascular calcification, is assessedusing one or more assays known to those of skill in the art. Exemplaryassays are described in Section 5.3(a)(iv). In accordance with suchembodiments, one of skill in the art will understand that a subjectbeing treated with an ActRII inhibitor as described herein may havetheir treatment regimen adjusted based on the outcome of the assays. Forexample, a subject being treated by a method described herein thatdisplays increases in levels of calcium, e.g., vascular calcium (e.g.,arterial calcium) may be administered an increased dose of ActRIIinhibitor, or a may be administered an ActRII inhibitor more frequently(i.e., the time between dose administrations may be decreased).Conversely, a subject being treated by a method described herein thatdisplays decreases in levels of calcium, e.g., vascular calcium (e.g.,arterial calcium) may be administered a decreased dose of ActRIIinhibitor, or a may be administered an ActRII inhibitor less frequently(i.e., the time between dose administrations may be increased).

In certain embodiments, the methods provided herein result in theimprovement of the symptoms of one or more of the following:hyperphosphatemia, secondary hyperparathyroidism (due to increase inphosphorus), and extraskeletal calcification, e.g., vascularcalcification. Any method known to the skilled artisan to determine thedegree of these symptoms can be used with the methods provided herein.In specific embodiments, the methods described herein result in theimprovement of one or more symptoms of vascular calcification. Exemplarysymptoms include, without limitation, increases in the levels ofvascular (e.g., arterial) calcium, increased apoptosis of vascularsmooth muscle cells, loss of arterial elasticity, an increase in PWV(pulse wave velocity), development of left ventricular hypertrophy,decrease in coronary artery perfusion, and myocardial ischaemia.

In certain embodiments, the methods described herein result in adecrease in the levels of vascular calcium, e.g., arterial calcium, in asubject by at least 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%.In certain embodiments, the methods described herein result in adecrease in the levels of vascular calcium, e.g., arterial calcium, in asubject by 5%-10%, 10%-15%, 15%-20%, 20%-25%, 25%-30%, 30%-35%, 35%-40%,40%-45%, or 45%-50%.

In a specific embodiment, provided herein is a method of reducing thelevels of vascular calcium in a subject, comprising: (i) administeringan ActRII inhibitor to a subject in need of reduction vascular calciumlevels (e.g., a subject having a form of CKD-MBD and/or extraskeletalcalcification, e.g., vascular calcification); (ii) determining an amountof vascular calcium in a tissue sample (e.g., serum) of said subjectafter the adminstration of the ActRII inhibitor; and (iii) if the amountof vascular calcium in said tissue sample is decreased by no more thanabout 5%, 10%, 15%, 20%, or 25%, or by about 5-10%, 10-20%, 20-30%, ascompared to the amount of vascular calcium determined in a sample of thesame tissue from said subject (e.g., a different sample of serum fromthe same individual) prior to administration of the ActRII inhibitor,repeating the administration of the ActRII inhibitor. In certainembodiments, if the amount of vascular calcium is not decreasedfollowing administration of the ActRII inhibitor, the dose of the ActRIIinhibitor administered can be increased. In certain embodiments, if theamount of vascular calcium is not decreased following administration ofthe ActRII inhibitor, the frequency of administration of the ActRIIinhibitor administered can be increased.

In certain embodiments, the methods described herein result in adecrease in the progression of the Agatston score of a subject having orat risk of developing vascular calcification. In a specific embodiment,the methods described herein result in a 5%, 10%, 15%, 20%, 25%, 30%, orgreater than 30% decrease in the Agatston score of a subject having orat risk of developing vascular calcification as compared to the Agatstonscore of the subject prior to administration of an ActRII inhibitor inaccordance with the methods described herein (see, e.g., Section5.3(a)(iv)). In another specific embodiment, the methods describedherein result in a 5%-10%, 10%-15%, 15%-20%, 20%-25%, 25%-30%, 30%-35%,35%-40%, 40%-45%, or 45%-50% decrease in the Agatston score of a subjecthaving or at risk of developing vascular calcification as compared tothe Agatston score of the subject prior to administration of an ActRIIinhibitor in accordance with the methods described herein (see, e.g.,Section 5.3(a)(iv)).

In another specific embodiment, the methods described herein result in adecrease in the levels of calcium in the vasculature of a subject, e.g.,a decrease in the levels of calcium in one or more arteries of thesubject, e.g., a subject having or at risk of developing vascularcalcification. In another specific embodiment, the methods describedherein result in a decrease in the levels of phosphorus in thevasculature of a subject, e.g., a decrease in the levels of phosphorusin one or more arteries of the subject, e.g., a subject having or atrisk of developing vascular calcification.

In certain embodiments, provided herein are methods for the treatment oflow turnover bone disorders. Low bone turnover can be diagnosed usingthe tests set forth in Section 5.3(a) below. Biochemical markers of boneturnover include: serum or urine collagen crosslinks (N-tclopcptidc orC-telopeptide), bone-specific alkaline phosphatase, serum osteocalcinand/or propeptide type 1 collagen, 25 hydroxyvitamin D, and parathyroidhormone (“PTH”). In a specific embodiment, the low turnover bonedisorder is adynamic bone disorder. In certain embodiments, a patient tobe treated with the methods provided herein has a reduction inbone-turnover of at least 10%, 20%, 25%, 30%, 40%, 50%, 60%, 70%, 75%,80%, 85%, 90%, 95%, 98%, 99% or of 100%. In certain embodiments, apatient to be treated with the methods provided herein has a reductionin bone-turnover of at most 10%, 20%, 25%, 30%, 40%, 50%, 60%, 70%, 75%,80%, 85%, 90%, 95%, 98%, 99% or of 100%. In certain embodiments, apatient to be treated with the methods provided herein has a reductionin bone-turnover of at between 10% and 25%, 20% and 35%, 30% and 45%,40% and 55%, 50% and 65%, 60% and 75%, 70% and 85%, 80% and 95%, 90% and100%. In certain embodiments, the reduction in bone turnover is comparedto historical data of the same patient. In other embodiments, thereduction in bone turnover is compared to the average bone turnover in apopulation without bone disorders. The population without bone disorderscan be of the same age and / or same sex as the patient.

In a specific embodiment, provided herein is a method of treating a lowturnover bone disorder, e.g., adynamic bone disorder, comprising: (i)administering an ActRII inhibitor to a subject having a low turnoverbone disorder; (ii) determining the level of bone-turnover in saidsubject after the adminstration of the ActRII inhibitor (e.g., by usingone or more of the tests set forth in Section 5.3(a) below and/or bymeasuring one or more biochemical markers of bone turnover); and (iii)if the level of bone turnover in the subject is decreased by no morethan about 5%, 10%, 15%, 20%, or 25%, or by about 5-10%, 10-20%, 20-30%,as compared to the level of bone turnover in the subject prior toadministration of the ActRII inhibitor, repeating the administration ofthe ActRII inhibitor. In certain embodiments, if the level of boneturnover is not decreased following administration of the ActRIIinhibitor, the dose of the ActRII inhibitor administered can beincreased. In certain embodiments, if the level of bone turnover is notdecreased following administration of the ActRII inhibitor, thefrequency of administration of the ActRII inhibitor administered can beincreased.

5.2 Inhibitors of ActRII (A) Inhibitors of ActRIIA

As used herein, the term “ActRIIA” refers to a family of activinreceptor type IIa (ActRIIA) proteins from any species and variantsderived from such ActRIIA proteins by mutagenesis or other modification.Reference to ActRIIA herein is understood to be a reference to any oneof the currently identified forms. Members of the ActRIIA family aregenerally transmembrane proteins, composed of a ligand-bindingextracellular domain with a cysteine-rich region, a transmembranedomain, and a cytoplasmic domain with predicted serine/threonine kinaseactivity.

ActRIIA inhibitors to be used in the compositions and methods describedherein include, without limitation, activin-binding soluble ActRIIApolypeptides; antibodies that bind to activin (particularly the activinA or B subunits, also referred to as ßA or ßB) and disrupt ActRIIAbinding; antibodies that bind to ActRIIA and disrupt activin binding;non-antibody proteins selected for activin or ActRIIA binding (see e.g.,WO/2002/088171, WO/2006/055689, WO/2002/032925, WO/2005/037989, US2003/0133939, and US 2005/0238646, each of which is incorporated hereinby reference in its entirety, for examples of such proteins and methodsfor design and selection of same); and randomized peptides selected foractivin or ActRIIA binding, which can be conjugated to an Fc domain.

In certain embodiments, two or more different proteins (or othermoieties) with activin or ActRIIA binding activity, especially activinbinders that block the type I (e.g., a soluble type I activin receptor)and type II (e.g., a soluble type II activin receptor) binding sites,respectively, may be linked together to create a bifunctional ormultifunctional binding molecule that inhibits ActRIIA and thus can beused in the compositions and methods described herein. In certainembodiments, Activin-ActRIIA signaling axis antagonists that inhibitActRIIA include nucleic acid aptamers, small molecules and other agentsare used in the compositions and methods described herein include.

(I) ActRIIA Inhibitors Comprising ActRIIA Polypeptides

The term “ActRIIA polypeptide” includes polypeptides comprising anynaturally occurring polypeptide of an ActRIIA family member as well asany variants thereof (including mutants, fragments, fusions, andpeptidomimetic forms) that retain a useful activity. For example,ActRIIA polypeptides include polypeptides derived from the sequence ofany known ActRIIA having a sequence at least about 80% identical to thesequence of an ActRIIA polypeptide, and optionally at least 85%, 90%,95%, 97%, 98%, 99% or greater identity. For example, an ActRIIApolypeptide may bind to and inhibit the function of an ActRIIA proteinand/or activin. An ActRIIB polypeptide may be selected for its abilityto promote bone growth and bone mineralization. Examples of ActRIIApolypeptides include human ActRIIA precursor polypeptide (SEQ ID NO: 1)and soluble human ActRIIA polypeptides (e.g., SEQ ID NOs: 2, 3, 7 and12). With respect to the ActRIIA precursor polypeptide whose amino acidsequence is depicted at SEQ ID NO:1, the signal peptide of the humanActRIIA precursor polypeptide located at amino acid positions 1 to 20;the extracellular domain is located at amino acid positions 21 to 135and the N-linked glycosylation sites of the human ActRIIA precursorpolypeptide (SEQ ID NO: 1) arc located at amino acid positions 43 and 56of SEQ ID NO:1. The nucleic acid sequence encoding the human ActRIIBprecursor polypeptide of SEQ ID NO:1 is disclosed as SEQ ID NO:4(nucleotides 164-1705 of Genbank entry NM_001616). The nucleic acidsequence encoding the soluble human ActRIIA polypeptide of SEQ ID NO:2is disclosed as SEQ ID NO:5. See Table 6 for a description of thesequences.

In specific embodiments, the ActRIIA polypeptides used in thecompositions and methods described herein are soluble ActRIIApolypeptides. An extracellular domain of an ActRIIA protein can bind toactivin and is generally soluble, and thus can be termed a soluble,activin-binding ActRIIA polypeptide. Thus, as used herein, the term“soluble ActRIIA polypeptide” generally refers to polypeptidescomprising an extracellular domain of an ActRIIA protein, including anynaturally occurring extracellular domain of an ActRIIA protein as wellas any variants thereof (including mutants, fragments and peptidomimeticforms). Soluble ActRIIA polypeptides can bind to activin; however, thewild type ActRIIA protein does not exhibit significant selectivity inbinding to activin versus GDF8/11. Native or altered ActRIIA proteinsmay be given added specificity for activin by coupling them with asecond, activin-selective binding agent. Examples of soluble,activin-binding ActRIIA polypeptides include the soluble polypeptidesillustrated in SEQ ID NOs: 2, 3, 7, 12 and 13. Other examples ofsoluble, activin-binding ActRIIA polypeptides comprise a signal sequencein addition to the extracellular domain of an ActRIIA protein, forexample, the honey bee mellitin leader sequence (SEQ ID NO: 8), thetissue plasminogen activator (TPA) leader (SEQ ID NO: 9) or the nativeActRIIA leader (SEQ ID NO: 10). The ActRIIA-hFc polypeptide illustratedin SEQ ID NO:13 uses a TPA leader.

In certain embodiments, the inhibitors of ActRIIA used in thecompositions and methods described herein comprise a conjugate/fusionprotein comprising an activin-binding domain of ActRIIA linked to an Fcportion of an antibody. In certain embodiments, the activin-bindingdomain is linked to an Fc portion of an antibody via a linker, e.g., apeptide linker. Optionally, the Fc domain has one or more mutations atresidues such as Asp-265, lysine 322, and Asn-434. In certain cases, themutant Fc domain having one or more of these mutations (e.g., an Asp-265mutation) has a reduced ability to bind to the Fcγ receptor relative toa wild-type Fc domain. In other cases, the mutant Fc domain having oneor more of these mutations (e.g., an Asn-434 mutation) has an increasedability to bind to the MHC class I- related Fc-receptor (FeRN) relativeto a wild-type Fc domain. Exemplary fusion proteins comprising a solubleextracellular domain of ActRIIA fused to an Fc domain are set forth inSEQ ID NOs: 6, 7, 12, and 13.

In a specific embodiment, the ActRIIA inhibitors used in thecompositions and methods described herein comprise the extracellulardomain of ActRIIA, or a portion thereof, linked to an Fc portion of anantibody, wherein said ActRIIA inhibitor comprises an amino acidsequence that is at least 75% identical to an amino acid sequenceselected from SEQ ID NOs: 6, 7, 12, and 13. In another specificembodiment, the ActRIIA inhibitors used in the compositions and methodsdescribed herein comprise the extracellular domain of ActRIIA, or aportion thereof, linked to an Fc portion of an antibody, wherein saidActRIIA inhibitor comprises an amino acid sequence that is at least 80%,85%, 90%, 95%, 96%, 97%, 98%, or 99% identical to an amino acid sequenceselected from SEQ ID NOs: 6, 7, 12, and 13.

In certain embodiments, the inhibitors of ActRIIA used in thecompositions and methods described herein comprise a truncated form ofan extracellular domain of ActRIIA. The truncation can be at the carboxyterminus and/or the amino terminus of the ActRIIA polypeptide. Incertain embodiments, the truncation can be 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 aminoacids long relative to the mature ActRIIB polypeptide extracellulardomain. In certain embodiments, the truncation can be 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or25 N-terminal amino acids of the mature ActRIIA polypeptideextracellular domain. In certain embodiments, the truncation can be 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, or 25 C-terminal amino acids of the mature ActRIIApolypeptide extracellular domain. For example, truncated forms ofActRIIA include polypeptides with amino acids 20-119; 20-128; 20-129;20-130; 20-131; 20-132; 20-133; 20-134; 20-131; 21-131; 22-131; 23-131;24-131; and 25-131, wherein the amino acid positions refer to the aminoacid positions in SEQ ID NO: 1.

In certain embodiments, the inhibitors of ActRIIA used in thecompositions and methods described herein comprise an extracellulardomain of ActRIIA with one or more amino acid substitutions. In certainembodiments, the inhibitors of ActRIIA used in the compositions andmethods described herein comprise a truncated form of an ActRIIAextracellular domain that also carries an amino acid substitution.

In a specific embodiment, the ActRIIA inhibitor to be used in thecompositions and methods described herein is a fusion protein betweenthe extracellular domain of the human ActRIIA receptor and the Fcportion of IgG1. In another specific embodiment, the ActRIIA inhibitorto be used in the compositions and methods described herein is a fusionprotein between a truncated extracellular domain of the human ActRIIAreceptor and the Fc portion of IgG1. In another specific embodiment, theActRIIA inhibitor to be used in the compositions and methods describedherein is a fusion protein between a truncated extracellular domain ofthe human ActRIIA receptor and the Fc portion of IgG1, wherein thetruncated extracellular domain of the human ActRIIA receptor possessesone or more amino acid substitutions.

Functionally active fragments of ActRIIA polypeptides can be obtained,for example, by screening polypeptides recombinantly produced from thecorresponding fragment of the nucleic acid encoding an ActRIIApolypeptide. In addition, fragments can be chemically synthesized usingtechniques known in the art such as conventional Merrifield solid phasef-Moc or t-Boc chemistry. The fragments can be produced (recombinantlyor by chemical synthesis) and tested to identify those peptidylfragments that can function as antagonists (inhibitors) of ActRIIAprotein or signaling mediated by activin.

In addition, functionally active variants of ActRIIA polypeptides can beobtained, for example, by screening libraries of modified polypeptidesrecombinantly produced from the corresponding mutagenized nucleic acidsencoding an ActRIIA polypeptide. The variants can be produced and testedto identify those that can function as antagonists (inhibitors) ofActRIIA protein or signaling mediated by activin. In certainembodiments, a functional variant of the ActRIIA polypeptides comprisesan amino acid sequence that is at least 75% identical to an amino acidsequence selected from SEQ ID NOs: 2 or 3. In certain cases, thefunctional variant has an amino acid sequence at least 80%, 85%, 90%,95%, 97%, 98%, 99% or 100% identical to an amino acid sequence selectedfrom SEQ ID NOs: 2 or 3.

Functional variants may be generated, for example, by modifying thestructure of an ActRIIA polypeptide for such purposes as enhancingtherapeutic efficacy, or stability (e.g., ex vivo shelf life andresistance to proteolytic degradation in vivo). Such modified ActRIIApolypeptides when selected to retain activin binding, can be consideredfunctional equivalents of the naturally-occurring ActRIIA polypeptides.Modified ActRIIA polypeptides can also be produced, for instance, byamino acid substitution, deletion, or addition. For instance, it isreasonable to expect that an isolated replacement of a leucine with anisoleucine or valine, an aspartate with a glutamate, a threonine with aserine, or a similar replacement of an amino acid with a structurallyrelated amino acid (e.g., conservative mutations) will not have a majoreffect on the biological activity of the resulting molecule.Conservative replacements are those that take place within a family ofamino acids that are related in their side chains. Whether a change inthe amino acid sequence of an ActRIIA polypeptide results in afunctional homolog can be readily determined by assessing the ability ofthe variant ActRIIA polypeptide to produce a response in cells in afashion similar to the wild-type ActRIIA polypeptide.

In certain embodiments, the ActRIIA inhibitor to be used in thecompositions and methods described herein may comprise an ActRIIApolypeptide having one or more specific mutations that can alter theglycosylation of the polypeptide. Such mutations may introduce oreliminate one or more glycosylation sites, such as O-linked or N-linkedglycosylation sites. Asparagine-linked glycosylation recognition sitesgenerally comprise a tripeptide sequence, asparagine-X-threonine (orasparagines-X-serine) (where “X” is any amino acid) which isspecifically recognized by appropriate cellular glycosylation enzymes.The alteration may also be made by the addition of, or substitution by,one or more serine or threonine residues to the sequence of thewild-type ActRIIA polypeptide (for O-linked glycosylation sites). Avariety of amino acid substitutions or deletions at one or both of thefirst or third amino acid positions of a glycosylation recognition site(and/or amino acid deletion at the second position) results innon-glycosylation at the modified tripeptide sequence. Another means ofincreasing the number of carbohydrate moieties on an ActRIIA polypeptideis by chemical or enzymatic coupling of glycosides to the ActRIIApolypeptide. Depending on the coupling mode used, the sugar(s) may beattached to (a) arginine and histidine; (b) free carboxyl groups; (c)free sulfhydryl groups such as those of cysteine; (d) free hydroxylgroups such as those of serine, threonine, or hydroxyproline; (e)aromatic residues such as those of phenylalanine, tyrosine, ortryptophan; or (f) the amide group of glutamine. These methods aredescribed in WO 87/05330 published Sep. 11, 1987, and in Aplin andWriston (1981) CRC Crit. Rev. Biochem., pp. 259-306, incorporated byreference herein. Removal of one or more carbohydrate moieties presenton an ActRIIA polypeptide may be accomplished chemically and/orenzymatically. Chemical deglycosylation may involve, for example,exposure of the ActRIIA polypeptide to the compoundtrifluoromethanesulfonic acid, or an equivalent compound. This treatmentresults in the cleavage of most or all sugars except the linking sugar(N-acetylglucosamine or N-acetylgalactosamine), while leaving the aminoacid sequence intact. Chemical deglycosylation is further described byHakimuddin et al. (1987) Arch. Biochem. Biophys. 259:52 and by Edge etal. (1981) Anal. Biochem. 118:131. Enzymatic cleavage of carbohydratemoieties on ActRIIA polypeptides can be achieved by the use of a varietyof endo- and exo-glycosidases as described by Thotakura et al. (1987)Meth. Enzymol. 138:350. The sequence of an ActRIIA polypeptide may beadjusted, as appropriate, depending on the type of expression systemused, as mammalian, yeast, insect and plant cells may all introducediffering glycosylation patterns that can be affected by the amino acidsequence of the peptide. In general, ActRIIA proteins for use in humanscan be expressed in a mammalian cell line that provides properglycosylation, such as HEK293 or CHO cell lines, although otherexpression systems, such as other mammalian expression cell lines, yeastcell lines with engineered glycosylation enzymes and insect cells, areexpected to be useful as well.

Further provided herein are methods of generating mutants, particularlysets of combinatorial mutants of an ActRIIA polypeptide, as well astruncation mutants; pools of combinatorial mutants are especially usefulfor identifying functional variant sequences. The purpose of screeningsuch combinatorial libraries may be to generate, for example, ActRIIApolypeptide variants which can act as either agonists or antagonist, oralternatively, which possess novel activities all together. A variety ofscreening assays are provided below, and such assays may be used toevaluate variants. For example, an ActRIIA polypeptide variant may bescreened for ability to bind to an ActRIIA ligand, to prevent binding ofan ActRIIA ligand to an ActRIIA polypeptide or to interfere withsignaling caused by an ActRIIA ligand.

Combinatorially-derived variants can be generated which have a selectiveor generally increased potency relative to a naturally occurring ActRIIApolypeptide. Likewise, mutagenesis can give rise to variants which haveintracellular half-lives dramatically different than the corresponding awild-type ActRIIA polypeptide. For example, the altered protein can berendered either more stable or less stable to proteolytic degradation orother cellular processes which result in destruction of, or otherwiseinactivation of a native ActRIIA polypeptide. Such variants, and thegenes which encode them, can be utilized to alter ActRIIA polypeptidelevels by modulating the half-life of the ActRIIA polypeptides. Forinstance, a short half-life can give rise to more transient biologicaleffects and can allow tighter control of recombinant ActRIIA polypeptidelevels within the patient. In an Fc fusion protein, mutations may bemade in the linker (if any) and/or the Fc portion to alter the half-lifeof the protein.

A combinatorial library may be produced by way of a degenerate libraryof genes encoding a library of polypeptides which each include at leasta portion of potential ActRIIA polypeptide sequences. For instance, amixture of synthetic oligonucleotides can be enzymatically ligated intogene sequences such that the degenerate set of potential ActRIIApolypeptide nucleotide sequences are expressible as individualpolypeptides, or alternatively, as a set of larger fusion proteins(e.g., for phage display).

There are many ways by which the library of potential homologs can begenerated from a degenerate oligonucleotide sequence. Chemical synthesisof a degenerate gene sequence can be carried out in an automatic DNAsynthesizer, and the synthetic genes then be ligated into an appropriatevector for expression. The synthesis of degenerate oligonucleotides iswell known in the art (see for example, Narang, S A (1983) Tetrahedron39:3; Itakura et al., (1981) Recombinant DNA, Proc. 3rd ClevelandSympos. Macromolecules, ed. AG Walton, Amsterdam: Elsevier pp 273-289;Itakura et al., (1984) Annu. Rev. Biochem. 53:323; Itakura et al.,(1984) Science 198:1056; Ike et al., (1983) Nucleic Acid Res. 11:477).Such techniques have been employed in the directed evolution of otherproteins (see, for example, Scott et al., (1990) Science 249:386-390;Roberts et al., (1992) PNAS USA 89:2429-2433; Devlin et al., (1990)Science 249: 404-406; Cwirla et al., (1990) PNAS USA 87: 6378-6382; aswell as U.S. Pat. Nos. 5,223,409, 5,198,346, and 5,096,815).

Alternatively, other forms of mutagenesis can be utilized to generate acombinatorial library. For example, ActRIIA polypeptide variants can begenerated and isolated from a library by screening using, for example,alanine scanning mutagenesis and the like (Ruf et al., (1994)Biochemistry 33:1565-1572; Wang et al., (1994) J. Biol. Chem.269:3095-3099; Balint et al., (1993) Gene 137:109-118; Grodberg et al.,(1993) Eur. J. Biochem. 218:597-601; Nagashima et al., (1993) J. Biol.Chem. 268:2888-2892; Lowman et al., (1991) Biochemistry 30:10832-10838;and Cunningham et al., (1989) Science 244:1081-1085), by linker scanningmutagenesis (Gustin et al., (1993) Virology 193:653-660; Brown et al.,(1992) Mol. Cell Biol. 12:2644-2652; McKnight et al., (1982) Science232:316); by saturation mutagenesis (Meyers et al., (1986) Science232:613); by PCR mutagenesis (Leung et al., (1989) Method Cell Mol Biol1:11-19); or by random mutagenesis, including chemical mutagenesis, etc.(Miller et al., (1992) A Short Course in Bacterial Genetics, CSHL Press,Cold Spring Harbor, N.Y.; and Greener et al., (1994) Strategies in MolBiol 7:32-34). Linker scanning mutagenesis, particularly in acombinatorial setting, is an attractive method for identifying truncated(bioactive) forms of ActRIIA polypeptides.

A wide range of techniques are known in the art for screening geneproducts of combinatorial libraries made by point mutations andtruncations, and, for that matter, for screening cDNA libraries for geneproducts having a certain property. Such techniques will be generallyadaptable for rapid screening of the gene libraries generated by thecombinatorial mutagenesis of ActRIIA polypeptides. The most widely usedtechniques for screening large gene libraries typically comprisescloning the gene library into replicable expression vectors,transforming appropriate cells with the resulting library of vectors,and expressing the combinatorial genes under conditions in whichdetection of a desired activity facilitates relatively easy isolation ofthe vector encoding the gene whose product was detected. Preferredassays include activin binding assays and activin-mediated cellsignaling assays.

In certain embodiments, ActRIIA polypeptides used in the inhibitors ofthe methods and compositions described herein may further comprisepost-translational modifications in addition to any that are naturallypresent in the ActRIIA polypeptides. Such modifications may include, butare not limited to, acetylation, carboxylation, glycosylation,phosphorylation, lipidation, and acylation. As a result, the modifiedActRIIA polypeptides may contain non-amino acid elements, such aspolyethylene glycols, lipids, poly- or mono-saccharide, and phosphates.Effects of such non-amino acid elements on the functionality of aActRIIA polypeptide may be tested by any method known to the skilledartisan. When an ActRIIA polypeptide is produced in cells by cleaving anascent form of the ActRIIA polypeptide, post-translational processingmay also be important for correct folding and/or function of theprotein. Different cells (such as CHO, HeLa, MDCK, 293, W138, NIH-3T3 orHEK293) have specific cellular machinery and characteristic mechanismsfor such post-translational activities and may be chosen to ensure thecorrect modification and processing of the ActRIIA polypeptides.

In certain aspects, functional variants or modified forms of the ActRIIApolypeptides used in the inhibitors of the methods and compositionsdescribed herein include fusion proteins having at least a portion ofthe ActRIIA polypeptides and one or more fusion domains. Well knownexamples of such fusion domains include, but are not limited to,polyhistidine, Glu-Glu, glutathione S transferase (GST), thioredoxin,protein A, protein G, an immunoglobulin heavy chain constant region(Fc), maltose binding protein (MBP), or human serum albumin. A fusiondomain may be selected so as to confer a desired property. For example,some fusion domains are particularly useful for isolation of the fusionproteins by affinity chromatography. For the purpose of affinitypurification, relevant matrices for affinity chromatography, such asglutathione-, amylase-, and nickel- or cobalt-conjugated resins areused. Many of such matrices are available in “kit” form, such as thePharmacia GST purification system and the QIAexpress.TM. system (Qiagen)useful with (HIS6) fusion partners. As another example, a fusion domainmay be selected so as to facilitate detection of the ActRIIApolypeptides. Examples of such detection domains include the variousfluorescent proteins (e.g., GFP) as well as “epitope tags,” which areusually short peptide sequences for which a specific antibody isavailable. Well known epitope tags for which specific monoclonalantibodies are readily available include FLAG, influenza virushemagglutinin (HA), and c-myc tags. In some cases, the fusion domainshave a protease cleavage site, such as for Factor Xa or Thrombin, whichallows the relevant protease to partially digest the fusion proteins andthereby liberate the recombinant proteins therefrom. The liberatedproteins can then be isolated from the fusion domain by subsequentchromatographic separation. In certain preferred embodiments, an ActRIIApolypeptide is fused with a domain that stabilizes the ActRIIApolypeptide in vivo (a “stabilizer” domain). By “stabilizing” is meantanything that increases serum half life, regardless of whether this isbecause of decreased destruction, decreased clearance by the kidney, orother pharmacokinetic effect. Fusions with the Fc portion of animmunoglobulin are known to confer desirable pharmacokinetic propertieson a wide range of proteins. Likewise, fusions to human serum albumincan confer desirable properties. Other types of fusion domains that maybe selected include multimerizing (e.g., dimerizing, tetramerizing)domains and functional domains (that confer an additional biologicalfunction, such as further stimulation of bone growth or muscle growth,as desired).

It is understood that different elements of the fusion proteins may bearranged in any manner that is consistent with the desiredfunctionality. For example, an ActRIIA polypeptide may be placedC-terminal to a heterologous domain, or, alternatively, a heterologousdomain may be placed C-terminal to an ActRIIA polypeptide. The ActRIIApolypeptide domain and the heterologous domain need not be adjacent in afusion protein, and additional domains or amino acid sequences may beincluded C- or N-terminal to either domain or between the domains.

In certain embodiments, the ActRIIA polypeptides used in the inhibitorsof the methods and compositions described herein may contain one or moremodifications that are capable of stabilizing the ActRIIA polypeptides.For example, such modifications may enhance the in vitro half life ofthe ActRIIA polypeptides, enhance circulatory half life of the ActRIIApolypeptides or reduce proteolytic degradation of the ActRIIApolypeptides. Such stabilizing modifications may include, but are notlimited to, fusion proteins (including, for example, fusion proteinscomprising an ActRIIA polypeptide and a stabilizer domain),modifications of a glycosylation site (including, for example, additionof a glycosylation site to an ActRIIA polypeptide), and modifications ofcarbohydrate moiety (including, for example, removal of carbohydratemoieties from an ActRIIA polypeptide). In the case of fusion proteins,an ActRIIA polypeptide is fused to a stabilizer domain such as an IgGmolecule (e.g., an Fc domain). As used herein, the term “stabilizerdomain” not only refers to a fusion domain (e.g., Fc) as in the case offusion proteins, but also includes nonproteinaceous modifications suchas a carbohydrate moiety, or nonproteinaceous polymer, such aspolyethylene glycol.

In certain embodiments, isolated and/or purified forms of ActRIIApolypeptides, which are isolated from, or otherwise substantially freeof other proteins can be used with the methods and compositionsdescribed herein. ActRIIA polypeptides can generally be produced byexpression from recombinant nucleic acids.

In certain aspects, the ActRIIA polypeptides used in the compositionsand methods described herein are generated using isolated and/orrecombinant nucleic acids encoding any of the ActRIIA polypeptides(e.g., soluble ActRIIA polypeptides), including fragments, functionalvariants and fusion proteins disclosed herein. For example, SEQ ID NO: 4encodes the naturally occurring human ActRIIA precursor polypeptide,while SEQ ID NO: 5 encodes the processed extracellular domain ofActRIIA. Such nucleic acids may be single-stranded or double stranded.Such nucleic acids may be DNA or RNA molecules. These nucleic acids maybe used, for example, in methods for making ActRIIA polypeptides or asdirect therapeutic agents (e.g., in a gene therapy approach).

In certain aspects, nucleic acids encoding ActRIIA polypeptides mayinclude nucleic acids that are variants of SEQ ID NO: 4 or 5. Variantnucleotide sequences include sequences that differ by one or morenucleotide substitutions, additions or deletions, such as allelicvariants.

In certain embodiments, isolated or recombinant nucleic acid sequencesencoding ActRIIA polypeptides may be least 80%, 85%, 90%, 95%, 97%, 98%,99% or 100% identical to SEQ ID NO: 4 or 5. One of ordinary skill in theart will appreciate that nucleic acid sequences complementary to SEQ IDNO: 4 or 5, and variants of SEQ ID NO: 4 or 5 may be used in theproduction of ActRIIA polypeptides suitable for use in the methods andcompositions described herein. In further embodiments, such nucleic acidsequences can be isolated, recombinant, and/or fused to a heterologousnucleotide sequence, or be from a DNA library.

In other embodiments, nucleic acids used in the production of ActRIIApolypeptides suitable for use in the methods and compositions describedherein may include nucleotide sequences that hybridize under highlystringent conditions to the nucleotide sequence designated in SEQ ID NO:4 or 5, complement sequence of SEQ ID NO: 4 or 5, or fragments thereof.One of ordinary skill in the art will understand that appropriatestringency conditions which promote DNA hybridization can be varied. Forexample, one can perform the hybridization at 6.0 times sodiumchloride/sodium citrate (SSC) at about 45 degree Celsius, followed by awash of 2.0 times SSC at 50 degree Celsius. For example, the saltconcentration in the wash step can be selected from a low stringency ofabout 2.0 times SSC at 50 degree Celsius to a high stringency of about0.2 times SSC at 50 degree Celsius. In addition, the temperature in thewash step can be increased from low stringency conditions at roomtemperature, about 22 degree Celsius, to high stringency conditions atabout 65 degree Celsius. Both temperature and salt may be varied, ortemperature or salt concentration may be held constant while the othervariable is changed. In one embodiment, nucleic acids which hybridizeunder low stringency conditions of 6 times SSC at room temperaturefollowed by a wash at 2 times SSC at room temperature can be used withthe methods and compositions described herein.

Isolated nucleic acids which differ from the nucleic acids as set forthin SEQ ID NOs: 4 or 5 due to degeneracy in the genetic code also can beused in the production of ActRIIA polypeptides suitable for use in themethods and compositions described herein. For example, a number ofamino acids are designated by more than one triplet. Codons that specifythe same amino acid, or synonyms (for example, CAU and CAC are synonymsfor histidine) may result in “silent” mutations which do not affect theamino acid sequence of the protein. However, it is expected that DNAsequence polymorphisms that do lead to changes in the amino acidsequences of the subject proteins will exist among mammalian cells. Oneskilled in the art will appreciate that these variations in one or morenucleotides (up to about 3-5% of the nucleotides) of the nucleic acidsencoding a particular protein may exist among individuals of a givenspecies due to natural allelic variation.

In certain embodiments, the recombinant nucleic acids may be operablylinked to one or more regulatory nucleotide sequences in an expressionconstruct. Regulatory nucleotide sequences will generally be appropriateto the host cell used for expression. Numerous types of appropriateexpression vectors and suitable regulatory sequences are known in theart for a variety of host cells. Typically, said one or more regulatorynucleotide sequences may include, but are not limited to, promotersequences, leader or signal sequences, ribosomal binding sites,transcriptional start and termination sequences, translational start andtermination sequences, and enhancer or activator sequences. Constitutiveor inducible promoters as known in the art are contemplated herein. Thepromoters may be either naturally occurring promoters, or hybridpromoters that combine elements of more than one promoter. An expressionconstruct may be present in a cell on an episome, such as a plasmid, orthe expression construct may be inserted in a chromosome. In a preferredembodiment, the expression vector contains a selectable marker gene toallow the selection of transformed host cells. Selectable marker genesare well known in the art and will vary with the host cell used.

In certain aspects, the a nucleic acid used in the production of ActRIIApolypeptides suitable for use in the methods and compositions describedherein can be provided in an expression vector comprising a nucleotidesequence encoding an ActRIIA polypeptide and operably linked to at leastone regulatory sequence. Regulatory sequences are art-recognized and areselected to direct expression of the ActRIIA polypeptide. Accordingly,the term regulatory sequence includes promoters, enhancers, and otherexpression control elements. Exemplary regulatory sequences aredescribed in Goeddel; Gene Expression Technology: Methods in Enzymology,Academic Press, San Diego, Calif. (1990). For instance, any of a widevariety of expression control sequences that control the expression of aDNA sequence when operatively linked to it may be used in these vectorsto express DNA sequences encoding an ActRIIA polypeptide. Such usefulexpression control sequences, include, for example, the early and latepromoters of SV40, tet promoter, adenovirus or cytomegalovirus immediateearly promoter, RSV promoters, the lac system, the trp system, the TACor TRC system, T7 promoter whose expression is directed by T7 RNApolymerase, the major operator and promoter regions of phage lambda, thecontrol regions for fd coat protein, the promoter for 3-phosphoglyceratekinase or other glycolytic enzymes, the promoters of acid phosphatase,e.g., Pho5, the promoters of the yeast α-mating factors, the polyhedronpromoter of the baculovirus system and other sequences known to controlthe expression of genes of prokaryotic or eukaryotic cells or theirviruses, and various combinations thereof. It should be understood thatthe design of the expression vector may depend on such factors as thechoice of the host cell to be transformed and/or the type of proteindesired to be expressed. Moreover, the vector’s copy number, the abilityto control that copy number and the expression of any other proteinencoded by the vector, such as antibiotic markers, should also beconsidered.

A recombinant nucleic acid used in the production of ActRIIApolypeptides suitable for use in the methods and compositions describedherein can be produced by ligating the cloned gene, or a portionthereof, into a vector suitable for expression in either prokaryoticcells, eukaryotic cells (yeast, avian, insect or mammalian), or both.Expression vehicles for production of a recombinant ActRIIA polypeptideinclude plasmids and other vectors. For instance, suitable vectorsinclude plasmids of the types: pBR322-derived plasmids, pEMBL-derivedplasmids, pEX-derived plasmids, pBTac-derived plasmids and pUC-derivedplasmids for expression in prokaryotic cells, such as E. coli.

Some mammalian expression vectors contain both prokaryotic sequences tofacilitate the propagation of the vector in bacteria, and one or moreeukaryotic transcription units that are expressed in eukaryotic cells.The pcDNAI/amp, pcDNAI/neo, pRc/CMV, pSV2gpt, pSV2neo, pSV2-dhfr, pTk2,pRSVnco, pMSG, pSVT7, pko-neo and pHyg derived vectors are examples ofmammalian expression vectors suitable for transfection of eukaryoticcells. Some of these vectors are modified with sequences from bacterialplasmids, such as pBR322, to facilitate replication and drug resistanceselection in both prokaryotic and eukaryotic cells. Alternatively,derivatives of viruses such as the bovine papilloma virus (BPV-1), orEpstein-Barr virus (pHEBo, pREP-derived and p205) can be used fortransient expression of proteins in eukaryotic cells. Examples of otherviral (including retroviral) expression systems can be found below inthe description of gene therapy delivery systems. The various methodsemployed in the preparation of the plasmids and in transformation ofhost organisms are well known in the art. For other suitable expressionsystems for both prokaryotic and eukaryotic cells, as well as generalrecombinant procedures, see Molecular Cloning A Laboratory Manual, 3rdEd., ed. by Sambrook, Fritsch and Maniatis (Cold Spring HarborLaboratory Press, 2001). In some instances, it may be desirable toexpress the recombinant polypeptides by the use of a baculovirusexpression system. Examples of such baculovirus expression systemsinclude pVL-derived vectors (such as pVL1392, pVL1393 and pVL941),pAcUW-derived vectors (such as pAcUW1), and pBlueBac-derived vectors(such as the β-gal containing pBlueBac III).

Vectors can be designed for production of the subject ActRIIApolypeptides in CHO cells, such as a Pcmv-Script vector (Stratagene, LaJolla, Calif.), pcDNA4 vectors (Invitrogen, Carlsbad, Calif.) andpCI-neo vectors (Promega, Madison, Wis.). As will be apparent, thesubject gene constructs can be used to cause expression of the subjectActRIIA polypeptides in cells propagated in culture, e.g., to produceproteins, including fusion proteins or variant proteins, forpurification.

Host cells transfected with a recombinant gene including a codingsequence (e.g., SEQ ID NO: 4 or 5) for one or more of the subjectActRIIA polypeptides can be used in the production of ActRIIApolypeptides suitable for use in the methods and compositions describedherein. The host cell may be any prokaryotic or eukaryotic cell. Forexample, an ActRIIA polypeptide provided herein may be expressed inbacterial cells such as E. coli, insect cells (e.g., using a baculovirusexpression system), yeast, or mammalian cells. Other suitable host cellsare known to those skilled in the art.

Accordingly, provided herein are methods of producing the ActRIIApolypeptides. For example, a host cell transfected with an expressionvector encoding an ActRIIA polypeptide can be cultured under appropriateconditions to allow expression of the ActRIIA polypeptide to occur. TheActRIIA polypeptide may be secreted and isolated from a mixture of cellsand medium containing the ActRIIA polypeptide. Alternatively, theActRIIA polypeptide may be retained cytoplasmically or in a membranefraction and the cells harvested, lysed and the protein isolated. A cellculture includes host cells, media and other byproducts. Suitable mediafor cell culture are well known in the art. The subject ActRIIApolypeptides can be isolated from cell culture medium, host cells, orboth, using techniques known in the art for purifying proteins,including ion-exchange chromatography, gel filtration chromatography,ultrafiltration, electrophoresis, immunoaffinity purification withantibodies specific for particular epitopes of the ActRIIA polypeptidesand affinity purification with an agent that binds to a domain fused tothe ActRIIA polypeptide (e.g., a protein A column may be used to purifyan ActRIIA-Fc fusion). In a preferred embodiment, the ActRIIApolypeptide is a fusion protein containing a domain which facilitatesits purification. In one embodiment, purification is achieved by aseries of column chromatography steps, including, for example, three ormore of the following, in any order: protein A chromatography, Qsepharose chromatography, phenylsepharose chromatography, size exclusionchromatography, and cation exchange chromatography. The purificationcould be completed with viral filtration and buffer exchange. Asdemonstrated herein, ActRIIA-hFc protein was purified to a purityof >98% as determined by size exclusion chromatography and >95% asdetermined by SDS PAGE. This level of purity was sufficient to achievedesirable effects on bone in mice and an acceptable safety profile inmice, rats and non-human primates.

In another embodiment, a fusion gene coding for a purification leadersequence, such as a poly-(His)/enterokinase cleavage site sequence atthe N-terminus of the desired portion of a recombinant ActRIIApolypeptide, can allow purification of the expressed fusion protein byaffinity chromatography using a Ni2+ metal resin. The purificationleader sequence can then be subsequently removed by treatment withenterokinase to provide the purified ActRIIA polypeptide (e.g., seeHochuli et al., (1987) J. Chromatography 411:177; and Janknecht et al.,PNAS USA 88:8972).

Techniques for making fusion genes are well known. Essentially, thejoining of various DNA fragments coding for different polypeptidesequences is performed in accordance with conventional techniques,employing blunt-ended or stagger-ended termini for ligation, restrictionenzyme digestion to provide for appropriate termini, filling-in ofcohesive ends as appropriate, alkaline phosphatase treatment to avoidundesirable joining, and enzymatic ligation. In another embodiment, thefusion gene can be synthesized by conventional techniques includingautomated DNA synthesizers. Alternatively, PCR amplification of genefragments can be carried out using anchor primers which give rise tocomplementary overhangs between two consecutive gene fragments which cansubsequently be annealed to generate a chimeric gene sequence (see, forexample, Current Protocols in Molecular Biology, eds. Ausubel et al.,John Wiley & Sons: 1992).

ActRIIA-Fc fusion protein can be expressed in stably transfectedCHO-DUKX Bl 1 cells from a pAID4 vector (SV40 ori/cnhanccr, CMVpromoter), using a tissue plasminogen leader sequence of SEQ ID NO:9.The Fc portion is a human IgG1 Fc sequence, as shown in SEQ ID NO:7. Incertain embodiments, upon expression, the protein contained has, onaverage, between about 1.5 and 2.5 moles of sialic acid per molecule ofActRIIA-Fc fusion protein.

In certain embodiments, the long serum half-life of an ActRIIA-Fc fusioncan be 25-32 days in human patients. Additionally, the CHO cellexpressed material can have a higher affinity for activin B ligand thanthat reported for an ActRIIA-hFc fusion protein expressed in human 293cells (del Re et al., J Biol Chem. 2004 Dec 17;279(51):53126-35).Additionally, without being bound by theory, the use of the TPA leadersequence provided greater production than other leader sequences and,unlike ActRIIA-Fc expressed with a native leader, may provide a highlypure N-terminal sequence. Use of the native leader sequence may resultin two major species of ActRIIA-Fc, each having a different N-terminalsequence.

(B) Inhibitors of ActRIIB

As used herein, the term “ActRIIB” refers to a family of activinreceptor type IIB (ActRIIB) proteins from any species and variantsderived from such ActRIIB proteins by mutagenesis or other modification.Reference to ActRIIB herein is understood to be a reference to any oneof the currently identified forms of the receptor. Members of theActRIIB family are generally transmembrane proteins, composed of aligand-binding extracellular domain with a cysteine-rich region, atransmembrane domain, and a cytoplasmic domain with predictedserine/threonine kinase activity.

ActRIIB inhibitors to be used in the compositions and methods describedherein include, without limitation, activin-binding soluble ActRIIBpolypeptides; antibodies that bind to activin (particularly the activinA or B subunits, also referred to as ßA or ßB) and disrupt ActRIIBbinding; antibodies that bind to ActRIIB and disrupt activin binding;non-antibody proteins selected for activin or ActRIIB binding; andrandomized peptides selected for activin or ActRIIB binding, which canbe conjugated to an Fc domain.

In certain embodiments, two or more different proteins (or othermoieties) with activin or ActRIIB binding activity, especially activinbinders that block the type I (e.g., a soluble type I activin receptor)and type II (e.g., a soluble type II activin receptor) binding sites,respectively, may be linked together to create a bifunctional ormultifunctional binding molecule that inhibits ActRIIB and thus can beused in the compositions and methods described herein include. Incertain embodiments, Activin-ActRIIB signaling axis antagonists thatinhibit ActRIIB include nucleic acid aptamers, small molecules and otheragents are used in the compositions and methods described hereininclude.

(I) ActRIIB Inhibitors Comprising ActRIIB Polypeptides

As used herein, the term “ActRIIB polypeptide” refers to polypeptidescomprising any naturally occurring polypeptide of an ActRIIB familymember as well as any variants thereof (including mutants, fragments,fusions, and peptidomimetic forms) that retain a useful activity. Forexample, ActRIIB polypeptides include polypeptides derived from thesequence of any known ActRIIB receptor having a sequence at least about80% identical to the sequence of an ActRIIB polypeptide, and optionallyat least 85%, 90%, 95%, 96%, 97%, 98%, 99% or greater identity. Forexample, an ActRIIB polypeptide may bind to and inhibit the function ofan ActRIIB protein and/or activin. An example of an ActRIIB polypeptideincludes the human ActRIIB precursor polypeptide (SEQ ID NO:16 or SEQ IDNO:28). With respect to the ActRIIB precursor polypeptide whose aminoacid sequence is depicted as SEQ ID NO:16 or SEQ ID NO:28 (i.e., thehuman ActRIIB precursor polypeptide), the signal peptide of the ActRIIBprecursor polypeptide is located at amino acids 1 to 18; theextracellular domain is located at amino acids 19 to 134 and thepotential N-linked glycosylation sites are located at amino acidpositions 42 and 65. The nucleic acid sequence encoding the humanActRIIB precursor polypeptide of SEQ ID NO:16 is disclosed as SEQ ID NO:19 (SEQ ID NO: 19 provides an alanine at the codon corresponding toamino acid position 64, but could be readily modified by one of skill inthe art using methods known in the art to provide an arginine at thecodon corresponding to amino acid position 64 instead). See Table 6 fora description of the sequences.

The numbering of amino acids for all of the ActRIIB-related polypeptidesdescribed herein is based on the amino acid numbering for SEQ ID NO:16and SEQ ID NO:28 (which only differ in the amino acid expressed atposition 64), unless specifically designated otherwise. For example, ifan ActRIIB polypeptide is described as having a substitution/mutation atamino acid position 79, then it is to be understood that position 79refers to the 79th amino acid in SEQ ID NO:16 or SEQ ID NO:28, fromwhich the ActRIIB polypeptide is derived. Likewise, if an ActRIIBpolypeptide is described as having an alanine or an arginine at aminoacid position 64, then it is to be understood that position 64 refers tothe 64th amino acid in SEQ ID NO:16 or SEQ ID NO:28, from which theActRIIB polypeptide is derived.

In certain embodiments, the inhibitors of ActRIIB used in thecompositions and methods described herein comprise polypeptidescomprising an activin-binding domain of ActRIIB. In some embodiments,the activin-binding domains of ActRIIB comprise the extracellular domainof ActRIIB, or a portion thereof. In specific embodiments, theextracellular domain or portion thereof of ActRIIB is soluble.Illustrative modified forms of ActRIIB polypeptides are disclosed inU.S. Pat. Application Publication Nos. 20090005308 and 20100068215, thedisclosures of which are incorporated herein by reference in theirentireties.

In specific embodiments, the ActRIIB polypeptides used in thecompositions and methods described herein are soluble ActRIIBpolypeptides. The term “soluble ActRIIB polypeptide” generally refers topolypeptides comprising an extracellular domain of an ActRIIB protein,including any naturally occurring extracellular domain of an ActRIIBprotein as well as any variants thereof (including mutants, fragmentsand peptidomimetic forms). Soluble ActRIIB polypeptides can bind toactivin; however, the wild type ActRIIB protein does not exhibitsignificant selectivity in binding to activin versus GDF8/11. In certainembodiments, altered forms of ActRIIB with different binding propertiescan be used in the methods provided herein. Such altered forms aredisclosed, e.g., in international patent application publication Nos. WO2006/012627 and WO 2010/019261, the disclosures of which areincorporated herein by reference in their entireties. Native or alteredActRIIB proteins may be given added specificity for activin by couplingthem with a second, activin-selective binding agent. Exemplary solubleActRIIB polypeptides include the extracellular domain of a human ActRIIBpolypeptide (e.g., SEQ ID NOs: 17, 18, 23, 26, 27, 29, 30, 31, 32, 33,36, 37, 42, and 43).

An Fc fusion protein having the ActRIIB extracellular sequence disclosedby Hilden et al. (Blood, 1994, 83(8):2163-70), which has an alanine atthe position corresponding to amino acid 64 of the ActRIIB precursoramino acid sequence, i.e., SEQ ID NO: 16 (herein referred to as “A64”),has been demonstrated to possess a relatively low affinity for activinand GDF-11. By contrast, an Fc fusion protein with an arginine atposition 64 of the ActRIIB precursor amino acid sequence (hereinreferred to as “R64”) has an affinity for activin and GDF-11 in the lownanomolar to high picomolar range (see, e.g., U.S. Pat. ApplicationPublication No. 20100068215, the disclosure of which is hereinincorporated in its entirety). An ActRIIB precursor amino acid sequencewith an arginine at position 64 is presented in SEQ ID NO:28. As such,in certain embodiments, the ActRIIB polypeptides used in accordance withthe compositions and methods described herein may comprise either (i) analanine at the position corresponding to amino acid 64 of the ActRIIBprecursor amino acid sequence, i.e., SEQ ID NO: 16; or (ii) an arginineat position 64 of the ActRIIB precursor amino acid sequence, i.e., SEQID NO: 28. In other embodiments, the ActRIIB polypeptides used inaccordance with the compositions and methods described herein maycomprise an amino acid that is not alanine or arginine at the positioncorresponding to amino acid 64 of the ActRIIB precursor amino acidsequence, i.e., SEQ ID NO: 16 or SEQ ID NO:28.

It has been shown that a deletion of the proline knot at the C-terminusof the extracellular domain of ActRIIB reduces the affinity of thereceptor for activin (see, e.g., Attisano et al., Cell, 1992,68(1):97-108). An ActRIIB-Fc fusion protein containing amino acids20-119 of SEQ ID NO: 28 (i.e., SEQ ID NO:32), “ActRIIB(20-119)-Fc” hasreduced binding to GDF-11 and activin relative to an ActRIIB-Fc fusionprotein containing amino acids 20-134 of SEQ ID NO: 28 (i.e., SEQ IDNO:31), “ActRIIB(20-134)-Fc”, which includes the proline knot region andthe complete juxtamembrane domain. However, an ActRIIB-Fc fusion proteincontaining amino acids 20-129 of SEQ ID NO: 28, “ActRIIB(20-129)-Fc”retains similar but somewhat reduced activity relative to thenon-truncated extracellular domain of ActRIIB, even though the prolineknot region is disrupted. Thus, ActRIIB polypeptides comprisingextracellular domains that stop at amino acid 134, 133, 132, 131, 130and 129 of SEQ ID NO: 28 (or SEQ ID NO:16) are all expected to beactive, but constructs stopping at amino acid 134 or 133 may be mostactive. Similarly, mutations at any of residues 129-134 are not expectedto alter ligand binding affinity by large margins, as indicated by thefact that mutations of P129 and P130 of SEQ ID NO: 28 do notsubstantially decrease ligand binding. Therefore, the ActRIIBpolypeptides used in accordance with the methods and compositionsdescribed herein may end as early as amino acid 109 (i.e., the finalcysteine) of SEQ ID NO:28 (or SEQ ID NO:16), however, forms ending at orbetween amino acid positions 109 and 119 of SEQ ID NO:28 (or SEQ IDNO:16) are expected to have reduced ligand binding ability.

Amino acid 29 of SEQ ID NO:16 and SEQ ID NO:28 represents the initialcysteine in the ActRIIB precursor sequence. It is expected that anActRIIB polypeptide beginning at amino acid 29 of the N-terminus of SEQID NO:16 or SEQ ID NO:28, or before these amino acid positions, willretain ligand binding activity. An alanine to asparagine mutation atposition 24 of SEQ ID NO:16 or SEQ ID NO:28 introduces an N-linkedglycosylation sequence without substantially affecting ligand binding.This confirms that mutations in the region between the signal cleavagepeptide and the cysteine cross-linked region, corresponding to aminoacids 20-29 of SEQ ID NO:16 or SEQ ID NO:28, are well tolerated. Inparticular, ActRIIB polypeptides beginning at amino acid position 20,21, 22, 23 and 24 of SEQ ID NO:16 or SEQ ID NO:28 will retain activity,and ActRIIB polypeptides beginning at amino acid positions 25, 26, 27,28 and 29 of SEQ ID NO:16 or SEQ ID NO:28 are also expected to retainactivity. An ActRIIB polypeptide beginning at amino acid position 22,23, 24 or 25 of SEQ ID NO:16 or SEQ ID NO:28 will have the mostactivity.

Taken together, the active portions (i.e., ActRIIB polypeptides) of theActRIIB precursor protein (i.e., SEQ ID NO:16 or SEQ ID NO:28) to beused in accordance with the methods and compositions described hereinwill generally comprise amino acids 29-109 of SEQ ID NO:16 or SEQ IDNO:28, and such ActRIIB polypeptides may, for example, begin at aresidue corresponding to any one of amino acids 19-29 of SEQ ID NO:16 orSEQ ID NO:28 and end at a position corresponding to any one of aminoacids 109-134 of SEQ ID NO:16 or SEQ ID NO:28. Specific examples ofActRIIB polypeptides encompassed herein include those that begin at anamino acid position from 19-29, 20-29 or 21-29 of SEQ ID NO:16 or SEQ IDNO:28 and end at an amino acid position from 119-134, 119-133 or129-134, 129-133 of SEQ ID NO:16 or SEQ ID NO:28. Other specificexamples of ActRIIB polypeptides encompassed herein include those thatbegin at an amino acid position from 20-24 (or 21-24, or 22-25) of SEQID NO:16 or SEQ ID NO:28 and end at an amino acid position from 109-134(or 109-133), 119-134 (or 119-133) or 129-134 (or 129-133) of SEQ IDNO:16 or SEQ ID NO:28. Variant ActRIIB polypeptides falling within theseranges are also contemplated, particularly those having at least 80%,85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequenceidentity or sequence homology to the corresponding portion of SEQ IDNO:16 or SEQ ID NO:28.

In certain embodiments, the inhibitors of ActRIIB used in thecompositions and methods described herein comprise a truncated form ofan extracellular domain of ActRIIB. The truncation can be at the carboxyterminus and/or the amino terminus of the ActRIIB polypeptide. Incertain embodiments, the truncation can be 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 aminoacids long relative to the mature ActRIIB polypeptide extracellulardomain. In certain embodiments, the truncation can be 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or25 N-terminal amino acids of the mature ActRIIB polypeptideextracellular domain. In certain embodiments, the truncation can be 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, or 25 C-terminal amino acids of the mature ActRIIBpolypeptide extracellular domain. For example, truncated forms ofActRIIB include polypeptides with amino acids 20-119; 20-128; 20-129;20-130; 20-131; 20-132; 20-133; 20-134; 20-131; 21-131; 22-131; 23-131;24-131; and 25-131, wherein the amino acid positions refer to the aminoacid positions in SEQ ID NO:16 or SEQ ID NO:28.

Additional exemplary truncated forms of ActRIIB include (i) polypeptidesbeginning at amino acids at any of amino acids 21-29 of SEQ ID NO:16 orSEQ ID NO:28 (optionally beginning at 22-25 of SEQ ID NO:16 or SEQ IDNO:28) and ending at any of amino acids 109-134 of SEQ ID NO:16 or SEQID NO:28; (ii) polypeptides beginning at any of amino acids 20-29 of SEQID NO:16 or SEQ ID NO:28 (optionally beginning at 22-25 of SEQ ID NO:16or SEQ ID NO:28) and ending at any of amino acids 109-133 of SEQ IDNO:16 or SEQ ID NO:28; (iii) polypeptides beginning at any of aminoacids 20-24 of SEQ ID NO:16 or SEQ ID NO:28 (optionally beginning at22-25 of SEQ ID NO:16 or SEQ ID NO:28) and ending at any of amino acids109-133 of SEQ ID NO:16 or SEQ ID NO:28; (iv) polypeptides beginning atany of amino acids 21-24 of SEQ ID NO:16 or SEQ ID NO:28 and ending atany of amino acids 109-134 of SEQ ID NO:16 or SEQ ID NO:28; (v)polypeptides beginning at any of amino acids 20-24 of SEQ ID NO:16 orSEQ ID NO:28 and ending at any of amino acids 118-133 of SEQ ID NO:16 orSEQ ID NO:28; (vi) polypeptides beginning at any of amino acids 21-24 ofSEQ ID NO:16 or SEQ ID NO:28 and ending at any of amino acids 118-134 ofSEQ ID NO:16 or SEQ ID NO:28; (vii) polypeptides beginning at any ofamino acids 20-24 of SEQ ID NO:16 or SEQ ID NO:28 and ending at any ofamino acids 128-133 of SEQ ID NO:16 or SEQ ID NO:28; (viii) polypeptidesbeginning at any of amino acids 20-24 of SEQ ID NO:16 or SEQ ID NO:28and ending at any of amino acids 128-133 of SEQ ID NO:16 or SEQ IDNO:28; (ix) polypeptides beginning at any of amino acids 21-29 of SEQ IDNO:16 or SEQ ID NO:28 and ending at any of amino acids 118-134 of SEQ IDNO:16 or SEQ ID NO:28; (x) polypeptides beginning at any of amino acids20-29 of SEQ ID NO:16 or SEQ ID NO:28 and ending at any of amino acids118-133 of SEQ ID NO:16 or SEQ ID NO:28; (xi) polypeptides beginning atany of amino acids 21-29 of SEQ ID NO:16 or SEQ ID NO:28 and ending atany of amino acids 128-134 of SEQ ID NO:16 or SEQ ID NO:28; and (xii)polypeptides beginning at any of amino acids 20-29 of SEQ ID NO:16 orSEQ ID NO:28 and ending at any of amino acids 128-133 of SEQ ID NO:16 orSEQ ID NO:28. In a specific embodiment, an ActRIIB polypeptidescomprises, consists essentially of, or consists of, an amino acidsequence beginning at amino acid position 25 of SEQ ID NO:16 or SEQ IDNO:28 and ending at amino acid position 131 of SEQ ID NO:16 or SEQ IDNO:28. In another specific embodiment, an ActRIIB polypeptide consistsof, or consists essentially of, the amino acid sequence of SEQ ID NO:17,18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, or 43.

Any of the ActRIIB polypeptides used in the compositions and methodsdescribed herein may be produced as a homodimer. Any of the ActRIIBpolypeptides used in the compositions and methods described herein maybe formulated as a fusion protein having a heterologous portion thatcomprises a constant region from an IgG heavy chain, such as an Fcdomain. Any of the ActRIIB polypeptides used in the compositions andmethods described herein may comprise an acidic amino acid at theposition corresponding to position 79 of SEQ ID NO:16 or SEQ ID NO:28,optionally in combination with one or more additional amino acidsubstitutions, deletions or insertions relative to SEQ ID NO:16 or SEQID NO:28.

In specific embodiments, the inhibitors of ActRIIB used in thecompositions and methods described herein comprise an extracellulardomain of ActRIIB with one or more amino acid substitutions/mutations.Such an amino acid substitution/mutation can be, for example, anexchange from the leucine at amino acid position 79 of SEQ ID NO:16 orSEQ ID NO:28 to an acidic amino acid, such as aspartic acid or glutamicacid. For example, position L79 of SEQ ID NO:16 or SEQ ID NO:28 may bealtered in ActRIIB extracellular domain polypeptides to confer alteredactivin-myostatin (GDF-11) binding properties. L79A and L79P mutationsreduce GDF-11 binding to a greater extent than activin binding. L79E andL79D mutations retain GDF-11 binding, while demonstrating greatlyreduced activin binding.

In certain embodiments, the inhibitors of ActRIIB used in thecompositions and methods described herein comprise a truncated form ofan ActRIIB extracellular domain that also carries an amino acidsubstitution, e.g., an exchange from the leucine at amino acid position79 of SEQ ID NO:16 or SEQ ID NO:28 to an acidic amino acid, such asaspartic acid or glutamic acid. In a specific embodiment, the truncatedform of an extracellular domain of ActRIIB polypeptide that also carriesan amino acid substitution used in the compositions and methodsdescribed herein is SEQ ID NO:23. Forms of ActRIIB that are truncatedand/or carry one or more amino acid substitutions can be linked to an Fcdomain of an antibody as discussed above.

Functionally active fragments of ActRIIB polypeptides can be obtained,for example, by screening polypeptides recombinantly produced from thecorresponding fragment of the nucleic acid encoding an ActRIIBpolypeptide. In addition, fragments can be chemically synthesized usingtechniques known in the art such as conventional Merrifield solid phasef-Moc or t-Boc chemistry. The fragments can be produced (recombinantlyor by chemical synthesis) and tested to identify those peptidylfragments that can function as antagonists (inhibitors) of ActRIIBprotein or signaling mediated by activin.

In addition, functionally active variants of ActRIIB polypeptides can beobtained, for example, by screening libraries of modified polypeptidesrecombinantly produced from the corresponding mutagenized nucleic acidsencoding an ActRIIB polypeptide. The variants can be produced and testedto identify those that can function as antagonists (inhibitors) ofActRIIB protein or signaling mediated by activin. In certainembodiments, a functional variant of the ActRIIB polypeptides comprisesan amino acid sequence that is at least 75% identical to an amino acidsequence selected from SEQ ID NO:17, 18, 23, 26, 27, 29, 30, 31, 32, 33,36, 37, 42, and 43. In certain embodiments, the functional variant hasan amino acid sequence at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or99% identical to an amino acid sequence selected from SEQ ID NO:17, 18,23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, and 43.

Functional variants may be generated, for example, by modifying thestructure of an ActRIIB polypeptide for such purposes as enhancingtherapeutic efficacy, or stability (e.g., ex vivo shelf life andresistance to proteolytic degradation in vivo). Such modified ActRIIBpolypeptides when selected to retain activin binding, are consideredfunctional equivalents of the naturally-occurring ActRIIB polypeptides.Modified ActRIIB polypeptides can also be produced, for instance, byamino acid substitution, deletion, or addition. For instance, it isreasonable to expect that an isolated replacement of a leucine with anisoleucine or valine, an aspartate with a glutamate, a threonine with aserine, or a similar replacement of an amino acid with a structurallyrelated amino acid (e.g., conservative mutations) will not have a majoreffect on the biological activity of the resulting molecule.Conservative replacements are those that take place within a family ofamino acids that are related in their side chains. Whether a change inthe amino acid sequence of an ActRIIB polypeptide results in afunctional homolog can be readily determined by assessing the ability ofthe variant ActRIIB polypeptide to produce a response in cells in afashion similar to the wild-type ActRIIB polypeptide.

ActRIIB polypeptide mutants, particularly sets of combinatorial mutantsof an ActRIIB polypeptide, as well as truncation mutants; pools ofcombinatorial mutants are especially useful for identifying functionalvariant sequences can be used in the methods and compositions describedherein. The purpose of screening such combinatorial libraries may be togenerate, for example, ActRIIB polypeptide variants which can act aseither agonists or antagonist, or alternatively, which possess novelactivities all together.

It has been demonstrated that the ligand binding pocket of ActRIIB isdefined by residues Y31, N33, N35, L38 through T41, E47, E50, Q53through K55, L57, H58, Y60, S62, K74, W78 through N83, Y85, R87, A92,and E94 through F101 of SEQ ID NO:16 or SEQ ID NO:28. At thesepositions, it is expected that conservative mutations will be tolerated,although a K74A mutation is well-tolerated, as are R40A, K55A, F82A andmutations at position L79. R40 is a K in Xenopus, indicating that basicamino acids at this position will be tolerated. Q53 is R in bovineActRIIB and K in Xenopus ActRIIB, and therefore amino acids including R,K, Q, N and H will be tolerated at this position. Thus, a generalformula for an ActRIIB polypeptide for use in the methods andcompositions described herein is one that comprises amino acids 29-109of SEQ ID NO:16 or SEQ ID NO:28, but optionally beginning at an aminoacid position ranging from 20-24 or 22-25 of SEQ ID NO:16 or SEQ IDNO:28 and ending at an amino acid position ranging from 129-134 of SEQID NO:16 or SEQ ID NO:28, and comprising no more than 1, 2, 5, or 15conservative amino acid changes in the ligand binding pocket, and zero,one or more non-conservative alterations at amino acid positions 40, 53,55, 74, 79 and/or 82 of SEQ ID NO:16 or SEQ ID NO:28 in the ligandbinding pocket. Such an ActRIIB polypeptide may retain greater than 80%,90%, 95% or 99% sequence identity or sequence homology to the sequenceof amino acids 29-109 of SEQ ID NO:16 or SEQ ID NO:28. Sites outside thebinding pocket, at which variability may be particularly well tolerated,include the amino and carboxy termini of the extracellular domain ofActRIIB, and positions 42-46 and 65-73. An asparagine to alaninealteration at position 65 of SEQ ID NO:16 or SEQ ID NO:28 (N65A)actually improves ligand binding in the A64 background, and is thusexpected to have no detrimental effect on ligand binding in the R64background. This change probably eliminates glycosylation at N65 in theA64 background, thus demonstrating that a significant change in thisregion is likely to be tolerated. While an R64A change is poorlytolerated, R64K is well-tolerated, and thus another basic residue, suchas H may be tolerated at position 64.

As a specific example of an ActRIIB polypeptide with a mutation in theligand binding domain, the positively-charged amino acid residue Asp(D80) of the ligand-binding domain of ActRIIB can be mutated to adifferent amino acid residue such that the variant ActRIIB polypeptidepreferentially binds to GDF8, but not activin. In a specific embodiment,the D80 residue is changed to an amino acid residue selected from thegroup consisting of: an uncharged amino acid residue, a negative aminoacid residue, and a hydrophobic amino acid residue. As a furtherspecific example, the hydrophobic residue L79 can be altered to theacidic amino acids aspartic acid or glutamic acid to greatly reduceactivin binding while retaining GDF11 binding. As will be recognized byone of skill in the art, most of the described mutations, variants ormodifications may be made at the nucleic acid level or, in some cases,by post translational modification or chemical synthesis. Suchtechniques are well known in the art.

In specific embodiments, the inhibitors of ActRIIB used in thecompositions and methods described herein comprise a conjugate/fusionprotein comprising an extracellular domain (e.g., an activin-bindingdomain) of an ActRIIB receptor linked to an Fc portion of an antibody.Such conjugate/fusion proteins may comprise any of the ActRIIBpolypeptides disclosed herein (e.g., any of SEQ ID NOs:17, 18, 23, 26,27, 29, 30, 31, 32, 33, 36, 37, 42, or 43), any ActRIIB polypeptidesknown in the art, or any ActRIIB polypeptides generated using methodsknown in the art and/or provided herein.

In certain embodiments, the extracellular domain is linked to an Fcportion of an antibody via a linker, e.g., a peptide linker. Exemplarylinkers include short polypeptide sequences such as 2-10, 2-5, 2-4, 2-3amino acid residues (e.g., glycine residues), such as, for example, aGly-Gly-Gly linker. In a specific embodiment, the linker comprises theamino acid sequence Gly-Gly-Gly (GGG). In another specific embodiment,the linker comprises the amino acid sequence Thr-Gly-Gly-Gly (TGGG).Optionally, the Fc domain has one or more mutations at residues such asAsp-265, lysine 322, and Asn-434. In certain cases, the mutant Fc domainhaving one or more of these mutations (e.g., an Asp-265 mutation) has areduced ability to bind to the Fcγ receptor relative to a wild-type Fcdomain. In other cases, the mutant Fc domain having one or more of thesemutations (e.g., an Asn-434 mutation) has an increased ability to bindto the MHC class I- related Fc-rcccptor (FeRN) relative to a wild-typeFc domain. Exemplary fusion proteins comprising a soluble extracellulardomain of ActRIIB fused to an Fc domain are set forth in SEQ ID NOs:20,21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46, and 47.

In a specific embodiment, the ActRIIB inhibitors used in thecompositions and methods described herein comprise the extracellulardomain of ActRIIB, or a portion thereof, linked to an Fc portion of anantibody, wherein said ActRIIB inhibitor comprises an amino acidsequence that is at least 75% identical to an amino acid sequenceselected from SEQ ID NOs:20, 21, 24, 25, 34, 35, 38, 39, 40, 41, 44, 46,and 47. In another specific embodiment, the ActRIIB inhibitors used inthe compositions and methods described herein comprise the extracellulardomain of ActRIIB, or a portion thereof, linked to an Fc portion of anantibody, wherein said ActRIIB inhibitor comprises an amino acidsequence that is at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%identical to an amino acid sequence selected from SEQ ID NOs:20, 21, 24,25, 34, 35, 38, 39, 40, 41, 44, 46, and 47.

In a specific embodiment, the ActRIIB inhibitor to be used in thecompositions and methods described herein is a fusion protein betweenthe extracellular domain of the human ActRIIB receptor and the Fcportion of IgG1. In another specific embodiment, the ActRIIB inhibitorto be used in the compositions and methods described herein is a fusionprotein between a truncated extracellular domain of the human ActRIIBreceptor and the Fc portion of IgG1. In another specific embodiment, theActRIIB inhibitor to be used in the compositions and methods describedherein is a fusion protein between a truncated extracellular domain ofthe human ActRIIB receptor and the Fc portion of IgG1, wherein thetruncated extracellular domain of the human ActRIIB receptor possessesan amino acid substitution at the amino acid position corresponding toamino acid 79 of SEQ ID NO:16 or SEQ ID NO:28. In one embodiment, theamino acid substitution at the amino acid position corresponding toamino acid 79 of SEQ ID NO:16 or SEQ ID NO:28 is substitution of Leucinefor Aspartic Acid (i.e., an L79D mutation).

In a specific embodiment, the ActRIIB inhibitor to be used in thecompositions and methods described herein is SEQ ID NO:24 or 25, whichrepresents a fusion protein between the extracellular domain of thehuman ActRIIB receptor and the Fc portion of IgG1, wherein said ActRIIBextracellular domain comprises amino acids 25-131 of SEQ ID NO:28 withan L79D mutation. The nucleic acid sequence encoding the ActRIIB-Fcfusion protein of SEQ ID NO:24 is presented in SEQ ID NO:45.

In another specific embodiment, the ActRIIB inhibitor to be used in thecompositions and methods described herein is SEQ ID NO:34 or 35, whichrepresents a fusion protein between the extracellular domain of thehuman ActRIIB receptor and the Fc portion of IgG1, wherein said ActRIIBextracellular domain comprises amino acids 25-131 of SEQ ID NO:16 withan L79D mutation.

Asparagine-linked glycosylation recognition sites generally comprise atripeptide sequence, asparagine-X-threonine (or asparagine-X-serine)(where “X” is any amino acid) which is specifically recognized byappropriate cellular glycosylation enzymes. The alteration may also bemade by the addition of, or substitution by, one or more serine orthreonine residues to the sequence of the wild-type ActRIIB polypeptide(for O-linked glycosylation sites). A variety of amino acidsubstitutions or deletions at one or both of the first or third aminoacid positions of a glycosylation recognition site (and/or amino aciddeletion at the second position) results in non-glycosylation at themodified tripeptide sequence. Another means of increasing the number ofcarbohydrate moieties on an ActRIIB polypeptide is by chemical orenzymatic coupling of glycosides to the ActRIIB polypeptide. Dependingon the coupling mode used, the sugar(s) may be attached to (a) arginineand histidine; (b) free carboxyl groups; (c) free sulfhydryl groups suchas those of cysteine; (d) free hydroxyl groups such as those of serine,threonine, or hydroxyproline; (e) aromatic residues such as those ofphenylalanine, tyrosine, or tryptophan; or (f) the amide group ofglutamine. These methods are described in International PatentApplication No. WO 87/05330 published Sep. 11, 1987, and in Aplin andWriston (1981) CRC Crit. Rev. Biochem., pp. 259-306, incorporated byreference herein. Removal of one or more carbohydrate moieties presenton an ActRIIB polypeptide may be accomplished chemically and/orenzymatically. Chemical deglycosylation may involve, for example,exposure of the ActRIIB polypeptide to the compoundtrifluoromethanesulfonic acid, or an equivalent compound. This treatmentresults in the cleavage of most or all sugars except the linking sugar(N-acetylglucosamine or N-acetylgalactosamine), while leaving the aminoacid sequence intact. Chemical deglycosylation is further described byHakimuddin et al. (1987) Arch. Biochem. Biophys. 259:52 and by Edge etal. (1981) Anal. Biochem. 118:131. Enzymatic cleavage of carbohydratemoieties on ActRIIB polypeptides can be achieved by the use of a varietyof endo-and exo-glycosidascs as described by Thotakura et al. (1987)Meth. Enzymol. 138:350. The sequence of an ActRIIB polypeptide may beadjusted, as appropriate, depending on the type of expression systemused, as mammalian, yeast, insect and plant cells may all introducediffering glycosylation patterns that can be affected by the amino acidsequence of the peptide. In general, ActRIIB proteins for use in humanswill be expressed in a mammalian cell line that provides properglycosylation, such as HEK293 or CHO cell lines, although otherexpression systems, such as other mammalian expression cell lines, yeastcell lines with engineered glycosylation enzymes and insect cells, areexpected to be useful as well.

In specific embodiments, mutated ActRIIB polypeptides comprising theaddition of a further N-linked glycosylation site (N-X-S/T) thatincreases the serum half-life of an ActRIIB-Fc fusion protein, relativeto the ActRIIB(R64)-Fc form can be used in the methods and compositionsdescribed herein. In a specific embodiment, introduction of anasparagine at position 24 of SEQ ID NO:16 or SEQ ID NO:28 (A24N) resultsin the creation of an NXT sequence that confers a longer half-life.Other NX(T/S) sequences can be found at 42-44 (NQS) and 65-67 (NSS),although the latter may not be efficiently glycosylated with the R atposition 64 (i.e., in R64 polypeptides). N-X-S/T sequences may begenerally introduced at positions outside the ligand binding pocket ofActRIIB, which is detailed above. Particularly suitable sites for theintroduction of non-endogenous N-X-S/T sequences include amino acids20-29, 20-24, 22-25, 109-134, 120-134 or 129-134 of SEQ ID NO:16 or SEQID NO:28. N-X-S/T sequences may also be introduced into the linkerbetween the ActRIIB sequence and the Fc or other fusion component. Sucha site may be introduced with minimal effort by introducing an N in thecorrect position with respect to a pre-existing S or T, or byintroducing an S or T at a position corresponding to a pre-existing N.Thus, desirable alterations that would create an N-linked glycosylationsite are: A24N, R64N, S67N (possibly combined with an N65A alteration),E106N, R112N, G120N, E123N, P129N, A132N, R112S and R112T (with allamino acid positions corresponding to the positions they can be found inSEQ ID NO:16 or SEQ ID NO:28). Any S that is predicted to beglycosylated may be altered to a T without creating an immunogenic site,because of the protection afforded by the glycosylation. Likewise, any Tthat is predicted to be glycosylated may be altered to an S. Thus thealterations S67T and S44T are encompassed herein. Likewise, in an A24Nvariant, an S26T alteration may be used. Accordingly, an ActRIIBpolypeptide may include one or more additional, non-endogenous N-linkedglycosylation consensus sequences.

A variety of screening assays may be used to evaluate ActRIIBpolypeptide variants. For example, an ActRIIB polypeptide variant may bescreened for ability to bind to an ActRIIB ligand, to prevent binding ofan ActRIIB ligand to an ActRIIB polypeptide or to interfere withsignaling caused by an ActRIIB ligand. The activity of an ActRIIBpolypeptide or its variants may also be tested in a cell-based or invivo assay.

Combinatorially-derived variants can be generated which have a selectiveor generally increased potency relative to a naturally occurring ActRIIBpolypeptide. Likewise, mutagenesis can give rise to variants which haveintracellular half-lives dramatically different than the correspondingwild-type ActRIIB polypeptide. For example, the altered protein can berendered either more stable or less stable to proteolytic degradation orother cellular processes which result in destruction of, or otherwiseinactivation of a native ActRIIB polypeptide. Such variants, and thegenes which encode them, can be utilized to alter ActRIIB polypeptidelevels by modulating the half-life of the ActRIIB polypeptides. Forinstance, a short half-life can give rise to more transient biologicaleffects and can allow tighter control of recombinant ActRIIB polypeptidelevels within the patient. In an Fc fusion protein, mutations may bemade in the linker (if any) and/or the Fc portion to alter the half-lifeof the protein.

A combinatorial library may be produced by way of a degenerate libraryof genes encoding a library of polypeptides which each include at leasta portion of potential ActRIIB polypeptide sequences. For instance, amixture of synthetic oligonucleotides can be enzymatically ligated intogene sequences such that the degenerate set of potential ActRIIBpolypeptide nucleotide sequences are expressible as individualpolypeptides, or alternatively, as a set of larger fusion proteins(e.g., for phage display).

There are many ways by which the library of potential homologs can begenerated from a degenerate oligonucleotide sequence. Chemical synthesisof a degenerate gene sequence can be carried out in an automatic DNAsynthesizer, and the synthetic genes then be ligated into an appropriatevector for expression. The synthesis of degenerate oligonucleotides iswell known in the art (see for example, Narang, S A (1983) Tetrahedron39:3; Itakura et al., (1981) Recombinant DNA, Proc. 3rd ClevelandSympos. Macromolecules, ed. AG Walton, Amsterdam: Elsevier pp 273-289;Itakura et al., (1984) Annu. Rev. Biochem. 53:323; Itakura et al.,(1984) Science 198:1056; Ike et al., (1983) Nucleic Acid Res. 11:477).Such techniques have been employed in the directed evolution of otherproteins (see, for example, Scott et al., (1990) Science 249:386-390;Roberts et al., (1992) PNAS USA 89:2429-2433; Devlin et al., (1990)Science 249: 404-406; Cwirla et al., (1990) PNAS USA 87: 6378-6382; aswell as U.S. Pat. Nos. 5,223,409, 5,198,346, and 5,096,815).

Alternatively, other forms of mutagenesis can be utilized to generate acombinatorial library. For example, ActRIIB polypeptide variants can begenerated and isolated from a library by screening using, for example,alanine scanning mutagenesis and the like (Ruf et al., (1994)Biochemistry 33:1565-1572; Wang et al., (1994) J. Biol. Chem.269:3095-3099; Balint et al., (1993) Gene 137:109-118; Grodberg et al.,(1993) Eur. J. Biochem. 218:597-601; Nagashima et al., (1993) J. Biol.Chem. 268:2888-2892; Lowman et al., (1991) Biochemistry 30:10832-10838;and Cunningham et al., (1989) Science 244:1081-1085), by linker scanningmutagenesis (Gustin et al., (1993) Virology 193:653-660; Brown et al.,(1992) Mol. Cell Biol. 12:2644-2652; McKnight et al., (1982) Science232:316); by saturation mutagenesis (Meyers et al., (1986) Science232:613); by PCR mutagenesis (Leung et al., (1989) Method Cell Mol Biol1:11-19); or by random mutagenesis, including chemical mutagenesis, etc.(Miller et al., (1992) A Short Course in Bacterial Genetics, CSHL Press,Cold Spring Harbor, N.Y.; and Greener et al., (1994) Strategies in MolBiol 7:32-34). Linker scanning mutagenesis, particularly in acombinatorial setting, is an attractive method for identifying truncated(bioactive) forms of ActRIIB polypeptides.

A wide range of techniques are known in the art for screening geneproducts of combinatorial libraries made by point mutations andtruncations, and, for that matter, for screening cDNA libraries for geneproducts having a certain property. Such techniques will be generallyadaptable for rapid screening of the gene libraries generated by thecombinatorial mutagenesis of ActRIIB polypeptides. The most widely usedtechniques for screening large gene libraries typically comprisescloning the gene library into replicable expression vectors,transforming appropriate cells with the resulting library of vectors,and expressing the combinatorial genes under conditions in whichdetection of a desired activity facilitates relatively easy isolation ofthe vector encoding the gene whose product was detected. Preferredassays include activin binding assays and activin-mediated cellsignaling assays.

In certain embodiments, ActRIIB polypeptides used in the methods andcompositions described herein may further comprise post-translationalmodifications in addition to any that are naturally present in theActRIIB polypeptides. Such modifications include, but are not limitedto, acetylation, carboxylation, glycosylation, phosphorylation,lipidation, and acylation. As a result, the modified ActRIIBpolypeptides may contain non-amino acid elements, such as polyethyleneglycols, lipids, poly- or mono-saccharide, and phosphates. Effects ofsuch non-amino acid elements on the functionality of a ActRIIBpolypeptide may be tested by any method known to the skilled artisan.When an ActRIIB polypeptide is produced in cells by cleaving a nascentform of the ActRIIB polypeptide, post-translational processing may alsobe important for correct folding and/or function of the protein.Different cells (such as CHO, HeLa, MDCK, 293, W138, NIH-3T3 or HEK293)have specific cellular machinery and characteristic mechanisms for suchpost-translational activities and may be chosen to ensure the correctmodification and processing of the ActRIIB polypeptides.

In certain aspects, functional variants or modified forms of the ActRIIBpolypeptides include fusion proteins having at least a portion of theActRIIB polypeptides and one or more fusion domains. Well known examplesof such fusion domains include, but are not limited to, polyhistidine,Glu-Glu, glutathione S transferase (GST), thioredoxin, protein A,protein G, an immunoglobulin heavy chain constant region (Fc), maltosebinding protein (MBP), or human serum albumin. A fusion domain may beselected so as to confer a desired property. For example, some fusiondomains are particularly useful for isolation of the fusion proteins byaffinity chromatography. For the purpose of affinity purification,relevant matrices for affinity chromatography, such as glutathione-,amylase-, and nickel- or cobalt-conjugated resins are used. Many of suchmatrices are available in “kit” form, such as the Pharmacia GSTpurification system and the QIAexpressTM system (Qiagen) useful with(HIS6) fusion partners. As another example, a fusion domain may beselected so as to facilitate detection of the ActRIIB polypeptides.Examples of such detection domains include the various fluorescentproteins (e.g., GFP) as well as “epitope tags,” which are usually shortpeptide sequences for which a specific antibody is available. Well knownepitope tags for which specific monoclonal antibodies are readilyavailable include FLAG, influenza virus hemagglutinin (HA), and c-myctags. In some cases, the fusion domains have a protease cleavage site,such as for Factor Xa or Thrombin, which allows the relevant protease topartially digest the fusion proteins and thereby liberate therecombinant proteins therefrom. The liberated proteins can then beisolated from the fusion domain by subsequent chromatographicseparation. In certain preferred embodiments, an ActRIIB polypeptide isfused with a domain that stabilizes the ActRIIB polypeptide in vivo (a“stabilizer” domain). By “stabilizing” is meant anything that increasesserum half life, regardless of whether this is because of decreaseddestruction, decreased clearance by the kidney, or other pharmacokineticeffect. Fusions with the Fc portion of an immunoglobulin are known toconfer desirable pharmacokinetic properties on a wide range of proteins.Likewise, fusions to human serum albumin can confer desirableproperties. Other types of fusion domains that may be selected includemultimerizing (e.g., dimerizing, tetramerizing) domains and functionaldomains (that confer an additional biological function, such as furtherstimulation of bone growth or muscle growth, as desired).

It is understood that different elements of the fusion proteins may bearranged in any manner that is consistent with the desiredfunctionality. For example, an ActRIIB polypeptide may be placedC-terminal to a heterologous domain, or, alternatively, a heterologousdomain may be placed C-terminal to an ActRIIB polypeptide. The ActRIIBpolypeptide domain and the heterologous domain need not be adjacent in afusion protein, and additional domains or amino acid sequences may beincluded C- or N-terminal to either domain or between the domains.

In certain embodiments, the ActRIIB polypeptides used in the methods andcompositions described herein contain one or more modifications that arecapable of stabilizing the ActRIIB polypeptides. For example, suchmodifications enhance the in vitro half life of the ActRIIBpolypeptides, enhance circulatory half life of the ActRIIB polypeptidesor reduce proteolytic degradation of the ActRIIB polypeptides. Suchstabilizing modifications include, but are not limited to, fusionproteins (including, for example, fusion proteins comprising an ActRIIBpolypeptide and a stabilizer domain), modifications of a glycosylationsite (including, for example, addition of a glycosylation site to anActRIIB polypeptide), and modifications of carbohydrate moiety(including, for example, removal of carbohydrate moieties from anActRIIB polypeptide). In the case of fusion proteins, an ActRIIBpolypeptide is fused to a stabilizer domain such as an IgG molecule(e.g., an Fc domain). As used herein, the term “stabilizer domain” notonly refers to a fusion domain (e.g., Fc) as in the case of fusionproteins, but also includes nonproteinaceous modifications such as acarbohydrate moiety, or nonproteinaceous polymer, such as polyethyleneglycol.

In certain embodiments, the methods and compositions described hereinuse isolated or purified ActRIIB polypeptides, i.e., ActRIIBpolypeptides which are isolated from, or otherwise substantially freeof, other proteins can be used with the methods and compositionsdescribed herein. ActRIIB polypeptides will generally be produced byexpression from recombinant nucleic acids.

In certain aspects, the ActRIIB polypeptides used in the methods andcompositions described herein are encoded by isolated and/or recombinantnucleic acids, including fragments, functional variants and fusionproteins disclosed herein. For example, SEQ ID NO:19 encodes thenaturally occurring human ActRIIB precursor polypeptide. The subjectnucleic acids may be single-stranded or double stranded. Such nucleicacids may be DNA or RNA molecules. These nucleic acids may be used, forexample, in methods for making ActRIIB polypeptides or as directtherapeutic agents (e.g., in a gene therapy approach).

In certain aspects, the nucleic acids that can be used to produceActRIIB polypeptides suitable for use in the methods and compositionsdescribed herein are further understood to include nucleic acids thatare variants of SEQ ID NO: 19 as well as variants of those nucleic acidsequences that encode soluble ActRIIB polypeptides (e.g., nucleic acidsthat encode SEQ ID NOs: 17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37,42, and 43). Variant nucleotide sequences include sequences that differby one or more nucleotide substitutions, additions or deletions, such asallelic variants.

In certain embodiments, the isolated or recombinant nucleic acidsequences that can be used to produce ActRIIB polypeptides suitable foruse in the methods and compositions described herein are at least 80%,85%, 90%, 95%, 97%, 98%, 99% or 100% identical to SEQ ID NO:19 or thosenucleic acid sequences that encode soluble ActRIIB polypeptides (e.g.,nucleic acids that encode SEQ ID NOs: 17, 18, 23, 26, 27, 29, 30, 31,32, 33, 36, 37, 42, and 43). One of ordinary skill in the art willappreciate that nucleic acid sequences complementary to SEQ ID NO:19 orthose nucleic acid sequences that encode soluble ActRIIB polypeptides(e.g., nucleic acids that encode SEQ ID NOs: 17, 18, 23, 26, 27, 29, 30,31, 32, 33, 36, 37, 42, and 43), and variants of SEQ ID NO:19 or thosenucleic acid sequences that encode soluble ActRIIB polypeptides (e.g.,nucleic acids that encode SEQ ID NOs: 17, 18, 23, 26, 27, 29, 30, 31,32, 33, 36, 37, 42, and 43) can be used with the methods andcompositions described herein. In further embodiments, the nucleic acidsequences can be isolated, recombinant, and/or fused with a heterologousnucleotide sequence, or in a DNA library.

In other embodiments, nucleic acids that can be used to produce ActRIIBpolypeptides suitable for use in the methods and compositions describedherein include nucleotide sequences that hybridize under highlystringent conditions to the nucleotide sequence designated in SEQ IDNO:19 or those nucleic acid sequences that encode soluble ActRIIBpolypeptides (e.g., nucleic acids that encode SEQ ID NOs: 17, 18, 23,26, 27, 29, 30, 31, 32, 33, 36, 37, 42, and 43), complement sequence ofSEQ ID NO:19 or those nucleic acid sequences that encode soluble ActRIIBpolypeptides (e.g., nucleic acids that encode SEQ ID NOs: 17, 18, 23,26, 27, 29, 30, 31, 32, 33, 36, 37, 42, and 43), or fragments thereof.One of ordinary skill in the art will understand that appropriatestringency conditions which promote DNA hybridization can be varied. Forexample, one can perform the hybridization at 6.0 times sodiumchloride/sodium citrate (SSC) at about 45 degree Celsius, followed by awash of 2.0 times SSC at 50 degree Celsius. For example, the saltconcentration in the wash step can be selected from a low stringency ofabout 2.0 times SSC at 50 degree Celsius to a high stringency of about0.2 times SSC at 50 degree Celsius. In addition, the temperature in thewash step can be increased from low stringency conditions at roomtemperature, about 22 degree Celsius, to high stringency conditions atabout 65 degree Celsius. Both temperature and salt may be varied, ortemperature or salt concentration may be held constant while the othervariable is changed. In one embodiment, nucleic acids which hybridizeunder low stringency conditions of 6 times SSC at room temperaturefollowed by a wash at 2 times SSC at room temperature can be used withthe methods and compositions described herein.

Isolated nucleic acids which differ from the nucleic acids as set forthin SEQ ID NO:19 or those nucleic acid sequences that encode solubleActRIIB polypeptides (e.g., nucleic acids that encode SEQ ID NOs: 17,18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, and 43) due todegeneracy in the genetic code can also be used to produce ActRIIBpolypeptides suitable for use in the methods and compositions describedherein. For example, a number of amino acids are designated by more thanone triplet. Codons that specify the same amino acid, or synonyms (forexample, CAU and CAC are synonyms for histidine) may result in “silent”mutations which do not affect the amino acid sequence of the protein.However, it is expected that DNA sequence polymorphisms that do lead tochanges in the amino acid sequences of the subject proteins will existamong mammalian cells. One skilled in the art will appreciate that thesevariations in one or more nucleotides (up to about 3-5% of thenucleotides) of the nucleic acids encoding a particular protein mayexist among individuals of a given species due to natural allelicvariation. Any and all such nucleotide variations and resulting aminoacid polymorphisms can be used with the methods and compositionsdescribed herein.

In certain embodiments, the recombinant nucleic acids that can be usedto produce ActRIIB polypeptides suitable for use in the methods andcompositions described herein may be operably linked to one or moreregulatory nucleotide sequences in an expression construct. Regulatorynucleotide sequences will generally be appropriate to the host cell usedfor expression. Numerous types of appropriate expression vectors andsuitable regulatory sequences are known in the art for a variety of hostcells. Typically, said one or more regulatory nucleotide sequences mayinclude, but are not limited to, promoter sequences, leader or signalsequences, ribosomal binding sites, transcriptional start andtermination sequences, translational start and termination sequences,and enhancer or activator sequences. Constitutive or inducible promotersas known in the art can be used with the methods and compositionsdescribed herein. The promoters may be either naturally occurringpromoters, or hybrid promoters that combine elements of more than onepromoter. An expression construct may be present in a cell on anepisome, such as a plasmid, or the expression construct may be insertedin a chromosome. In a preferred embodiment, the expression vectorcontains a selectable marker gene to allow the selection of transformedhost cells. Selectable marker genes are well known in the art and willvary with the host cell used.

In certain aspects, the nucleic acids that can be used to produceActRIIB polypeptides suitable for use in the methods and compositionsdescribed herein are provided in an expression vector comprising anucleotide sequence encoding an ActRIIB polypeptide and operably linkedto at least one regulatory sequence. Regulatory sequences areart-recognized and are selected to direct expression of the ActRIIBpolypeptide. Accordingly, the term regulatory sequence includespromoters, enhancers, and other expression control elements. Exemplaryregulatory sequences are described in Goeddel; Gene ExpressionTechnology: Methods in Enzymology, Academic Press, San Diego, Calif.(1990). For instance, any of a wide variety of expression controlsequences that control the expression of a DNA sequence when operativelylinked to it may be used in these vectors to express DNA sequencesencoding an ActRIIB polypeptide. Such useful expression controlsequences, include, for example, the early and late promoters of SV40,tet promoter, adenovirus or cytomegalovirus immediate early promoter,RSV promoters, the lac system, the trp system, the TAC or TRC system, T7promoter whose expression is directed by T7 RNA polymerase, the majoroperator and promoter regions of phage lambda, the control regions forfd coat protein, the promoter for 3-phosphoglycerate kinase or otherglycolytic enzymes, the promoters of acid phosphatase, e.g., Pho5, thepromoters of the yeast α-mating factors, the polyhedron promoter of thebaculovirus system and other sequences known to control the expressionof genes of prokaryotic or eukaryotic cells or their viruses, andvarious combinations thereof. It should be understood that the design ofthe expression vector may depend on such factors as the choice of thehost cell to be transformed and/or the type of protein desired to beexpressed. Moreover, the vector’s copy number, the ability to controlthat copy number and the expression of any other protein encoded by thevector, such as antibiotic markers, should also be considered.

A recombinant nucleic acid can be produced by ligating the cloned gene,or a portion thereof, into a vector suitable for expression in eitherprokaryotic cells, eukaryotic cells (yeast, avian, insect or mammalian),or both. Expression vehicles for production of a recombinant ActRIIBpolypeptide include plasmids and other vectors. For instance, suitablevectors include plasmids of the types: pBR322-derived plasmids,pEMBL-derived plasmids, pEX-derived plasmids, pBTac-derived plasmids andpUC-derived plasmids for expression in prokaryotic cells, such as E.coli.

Some mammalian expression vectors contain both prokaryotic sequences tofacilitate the propagation of the vector in bacteria, and one or moreeukaryotic transcription units that are expressed in eukaryotic cells.The pcDNAI/amp, pcDNAI/neo, pRc/CMV, pSV2gpt, pSV2neo, pSV2-dhfr, pTk2,pRSVnco, pMSG, pSVT7, pko-nco and pHyg derived vectors arc examples ofmammalian expression vectors suitable for transfection of eukaryoticcells. Some of these vectors are modified with sequences from bacterialplasmids, such as pBR322, to facilitate replication and drug resistanceselection in both prokaryotic and eukaryotic cells. Alternatively,derivatives of viruses such as the bovine papilloma virus (BPV-1), orEpstein-Barr virus (pHEBo, pREP-derived and p205) can be used fortransient expression of proteins in eukaryotic cells. Examples of otherviral (including retroviral) expression systems can be found below inthe description of gene therapy delivery systems. The various methodsemployed in the preparation of the plasmids and in transformation ofhost organisms arc well known in the art. For other suitable expressionsystems for both prokaryotic and eukaryotic cells, as well as generalrecombinant procedures, see Molecular Cloning A Laboratory Manual, 3rdEd., ed. by Sambrook, Fritsch and Maniatis (Cold Spring HarborLaboratory Press, 2001). In some instances, it may be desirable toexpress the recombinant polypeptides by the use of a baculovirusexpression system. Examples of such baculovirus expression systemsinclude pVL-derived vectors (such as pVL1392, pVL1393 and pVL941),pAcUW-derived vectors (such as pAcUW1), and pBlueBac-derived vectors(such as the β-gal containing pBlueBac III).

In one embodiment, a vector can be designed for production of theActRIIB polypeptides used in the methods and compositions describedherein in CHO cells, such as a Pcmv-Script vector (Stratagene, La Jolla,Calif.), pcDNA4 vectors (Invitrogen, Carlsbad, Calif.) and pCI-neovectors (Promega, Madison, Wis.). As will be apparent, the subject geneconstructs can be used to cause expression of the subject ActRIIBpolypeptides in cells propagated in culture, e.g., to produce proteins,including fusion proteins or variant proteins, for purification.

Host cells transfected with a recombinant gene including a codingsequence (e.g., SEQ ID NO:19 or those nucleic acid sequences that encodesoluble ActRIIB polypeptides (e.g., nucleic acids that encode SEQ IDNOs: 17, 18, 23, 26, 27, 29, 30, 31, 32, 33, 36, 37, 42, and 43)) forone or more of the subject ActRIIB polypeptides can be used to produceActRIIB polypeptides suitable for use in the methods and compositionsdescribed herein. The host cell may be any prokaryotic or eukaryoticcell. For example, an ActRIIB polypeptide may be expressed in bacterialcells such as E. coli, insect cells (e.g., using a baculovirusexpression system), yeast, or mammalian cells. Other suitable host cellsare known to those skilled in the art.

Accordingly, provided herein arc methods of producing the ActRIIBpolypeptides used in the methods and compositions described herein. Forexample, a host cell transfected with an expression vector encoding anActRIIB polypeptide can be cultured under appropriate conditions toallow expression of the ActRIIB polypeptide to occur. The ActRIIBpolypeptide may be secreted and isolated from a mixture of cells andmedium containing the ActRIIB polypeptide. Alternatively, the ActRIIBpolypeptide may be retained cytoplasmically or in a membrane fractionand the cells harvested, lysed and the protein isolated. A cell cultureincludes host cells, media and other byproducts. Suitable media for cellculture are well known in the art. The subject ActRIIB polypeptides canbe isolated from cell culture medium, host cells, or both, usingtechniques known in the art for purifying proteins, includingion-exchange chromatography, gel filtration chromatography,ultrafiltration, electrophoresis, immunoaffinity purification withantibodies specific for particular epitopes of the ActRIIB polypeptidesand affinity purification with an agent that binds to a domain fused tothe ActRIIB polypeptide (e.g., a protein A column may be used to purityan ActRIIB-Fc fusion). In a preferred embodiment, the ActRIIBpolypeptide is a fusion protein containing a domain which facilitatesits purification. In a preferred embodiment, purification is achieved bya series of column chromatography steps, including, for example, threeor more of the following, in any order: protein A chromatography, Qsepharose chromatography, phenylsepharose chromatography, size exclusionchromatography, and cation exchange chromatography. The purificationcould be completed with viral filtration and buffer exchange. Asdemonstrated herein, ActRIIB -hFc protein was purified to a purityof >98% as determined by size exclusion chromatography and >95% asdetermined by SDS PAGE. This level of purity was sufficient to achievedesirable effects on bone in mice and an acceptable safety profile inmice, rats and non-human primates.

In another embodiment, a fusion gene coding for a purification leadersequence, such as a poly-(His)/enterokinase cleavage site sequence atthe N-terminus of the desired portion of the recombinant ActRIIBpolypeptide, can allow purification of the expressed fusion protein byaffinity chromatography using a Ni2+ metal resin. The purificationleader sequence can then be subsequently removed by treatment withenterokinase to provide the purified ActRIIB polypeptide (e.g., seeHochuli et al., (1987) J. Chromatography 411:177; and Janknecht et al.,PNAS USA 88:8972).

Techniques for making fusion genes are well known. Essentially, thejoining of various DNA fragments coding for different polypeptidesequences is performed in accordance with conventional techniques,employing blunt-ended or stagger-ended termini for ligation, restrictionenzyme digestion to provide for appropriate termini, filling-in ofcohesive ends as appropriate, alkaline phosphatase treatment to avoidundesirable joining, and enzymatic ligation. In another embodiment, thefusion gene can be synthesized by conventional techniques includingautomated DNA synthesizers. Alternatively, PCR amplification of genefragments can be carried out using anchor primers which give rise tocomplementary overhangs between two consecutive gene fragments which cansubsequently be annealed to generate a chimeric gene sequence (see, forexample, Current Protocols in Molecular Biology, cds. Ausubcl et al.,John Wiley & Sons: 1992).

ActRIIB -Fc fusion protein can be expressed in stably transfectedCHO-DUKX B1 1 cells from a pAID4 vector (SV40 ori/enhancer, CMVpromoter), using a tissue plasminogen leader sequence of SEQ ID NO:8.The Fc portion can comprise a human IgGl Fc sequence, as shown in SEQ IDNO:7. In certain embodiments, upon expression, the protein containedhas, on average, between about 1.5 and 2.5 moles of sialic acid permolecule of ActRIIB-Fc fusion protein.

In certain embodiments, the long serum half-life of an ActRIIB-Fc fusioncan be 25-32 days in human patients. Additionally, the CHO cellexpressed material can have a higher affinity for activin B ligand thanthat reported for an ActRIIB-hFc fusion protein expressed in human 293cells (del Re et al., J Biol Chem. 2004 Dec 17;279(51):53126-35).Additionally, without being bound by theory, the use of the TPA leadersequence provided greater production than other leader sequences and,unlike ActRIIB-Fc expressed with a native leader, may provide a highlypure N-terminal sequence. Use of the native leader sequence may resultin two major species of ActRIIB-Fc, each having a different N-terminalsequence.

(II) Other ActRII Receptor Inhibitors

In certain embodiments, the inhibitors of ActRII receptors used in thecompositions and methods described herein are nucleic acid compounds.

Examples of categories of nucleic acid compounds that inhibit ActRIIreceptors include antisense nucleic acids, siRNA or RNAi constructs andcatalytic nucleic acid constructs. A nucleic acid compound may besingle- or double-stranded. A double-stranded compound may also includeregions of overhang or non-complementarity, where one or the other ofthe strands is single-stranded. A single-stranded compound may includeregions of self-complementarity, meaning that the compound may form aso-called “hairpin” or “stem-loop” structure, with a region of doublehelical structure.

In certain embodiments, the nucleic acid compounds that inhibit ActRIIreceptors may comprise a nucleotide sequence that is complementary to aregion consisting of no more than 1000, no more than 500, no more than250, no more than 100 or no more than 50, 35, 30, 25, 22, 20 or 18nucleotides of the full-length ActRII receptor nucleic acid sequence oractivin nucleic acid sequence (e.g., the nucleic acid sequence of anactivin A or activin B subunit, also referred to as βA or βB). Inspecific embodiments, the region of complementarity will be at least 8nucleotides, and optionally at least 10 or at least 15 nucleotides, andoptionally between 15 and 25 nucleotides. A region of complementaritymay fall within an intron, a coding sequence or a noncoding sequence ofthe target transcript, such as the coding sequence portion. Generally, anucleic acid compound that inhibits an ActRII receptor will have alength of about 8 to about 500 nucleotides or base pairs in length, andoptionally the length will be about 14 to about 50 nucleotides. Anucleic acid compound that inhibits an ActRII receptor may be a DNA(particularly for use as an antisense), an RNA, or an RNA:DNA hybrid.Any one strand may include a mixture of DNA and RNA, as well as modifiedforms that cannot readily be classified as either DNA or RNA. Likewise,a double stranded nucleic acid compound may be DNA:DNA, DNA:RNA, orRNA:RNA, and any one strand may also include a mixture of DNA and RNA,as well as modified forms that cannot readily be classified as eitherDNA or RNA.

The nucleic acid compounds that inhibit an ActRII receptor may includeany of a variety of modifications, including one or modifications to thebackbone (the sugar-phosphate portion in a natural nucleic acid,including internucleotide linkages) or the base portion (the purine orpyrimidine portion of a natural nucleic acid). In certain embodiments,an antisense nucleic acid compound will have a length of about 15 toabout 30 nucleotides and will often contain one or more modifications toimprove certain characteristics, such as stability in the serum,stability in a cell, or stability in a place where the compound islikely to be delivered, such as, e.g., the stomach in the case of orallydelivered compounds and the lung for inhaled compounds. In the case ofan RNAi construct, the strand complementary to the target transcriptwill generally be RNA or modifications thereof. The other strand may beRNA, DNA, or any other variation. The duplex portion of double strandedor single stranded “hairpin” RNAi construct may, in certain embodiments,have a length of 18 to 40 nucleotides in length and optionally about 21to 23 nucleotides in length, so long as it serves as a Dicer substrate.Catalytic or enzymatic nucleic acids may be ribozymes or DNA enzymes andmay also contain modified forms. In certain embodiments, nucleic acidcompounds that inhibit ActRII receptors may inhibit expression of theirtarget by about 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or moreunder physiological conditions and at a concentration where a nonsenseor sense control has little or no effect. Concentrations for testing theeffect of nucleic acid compounds include 1, 5, 10 micromolar, or more.

In other embodiments, the inhibitors of ActRII receptors used in thecompositions and methods described herein arc antibodies. Suchantibodies include antibodies that bind to activin (particularly theactivin A or B subunits, also referred to as βA or βB) and disruptActRII receptor binding; and antibodies that bind to ActRII receptorpolypeptides (e.g., a soluble ActRIIA or soluble ActRIIB polypeptide)and disrupt activin binding.

By using immunogens derived from an ActRII receptor polypeptide or anactivin polypeptide, anti-protein/anti-peptide antisera or monoclonalantibodies can be made by standard protocols (see, for example,Antibodies: A Laboratory Manual ed. by Harlow and Lane (Cold SpringHarbor Press: 1988)). A mammal, such as a mouse, a hamster or rabbit canbe immunized with an immunogenic form of the ActRII receptorpolypeptide, an antigenic fragment which is capable of eliciting anantibody response, or a fusion protein. Techniques for conferringimmunogenicity on a protein or peptide include conjugation to carriersor other techniques well known in the art. An immunogenic portion of anActRII receptor or activin polypeptide can be administered in thepresence of adjuvant. The progress of immunization can be monitored bydetection of antibody titers in plasma or serum. Standard ELISA or otherimmunoassays can be used with the immunogen as antigen to assess thelevels of antibodies.

Following immunization of an animal with an antigenic preparation of anActRII receptor polypeptide, antisera can be obtained and, if desired,polyclonal antibodies can be isolated from the serum. To producemonoclonal antibodies, antibody-producing cells (lymphocytes) can beharvested from an immunized animal and fused by standard somatic cellfusion procedures with immortalizing cells such as myeloma cells toyield hybridoma cells. Such techniques are well known in the art, andinclude, for example, the hybridoma technique (originally developed byKohler and Milstein, (1975) Nature, 256: 495-497), the human B cellhybridoma technique (Kozbar et al., (1983) Immunology Today, 4: 72), andthe EBV-hybridoma technique to produce human monoclonal antibodies (Coleet al., (1985) Monoclonal Antibodies and Cancer Therapy, Alan R. Liss,Inc. pp. 77-96). Hybridoma cells can be screened immunochemically forproduction of antibodies specifically reactive with an ActRII receptorpolypeptide and monoclonal antibodies isolated from a culture comprisingsuch hybridoma cells.

The term “antibody” as used herein is intended to include fragmentsthereof which are also specifically reactive with a subject polypeptide.Antibodies can be fragmented using conventional techniques and thefragments screened for utility in the same manner as described above forwhole antibodies. For example, F(ab)2 fragments can be generated bytreating antibody with pepsin. The resulting F(ab)2 fragment can betreated to reduce disulfide bridges to produce Fab fragments. Anantibody is further intended to include bispecific, single-chain,chimeric, humanized and fully human molecules having affinity for anActRII receptor or activin polypeptide conferred by at least one CDRregion of the antibody. An antibody may further comprise a labelattached thereto and able to be detected (e.g., the label can be aradioisotope, fluorescent compound, enzyme or enzyme co-factor).

In certain embodiments, the antibody is a recombinant antibody, whichterm encompasses any antibody generated in part by techniques ofmolecular biology, including CDR-grafted or chimeric antibodies, humanor other antibodies assembled from library-selected antibody domains,single chain antibodies and single domain antibodies (e.g., human VHproteins or camelid VHH proteins). In certain embodiments, an antibodycan be a monoclonal antibody, and in certain embodiments. For example, amethod for generating a monoclonal antibody that binds specifically toan ActRII receptor polypeptide or activin polypeptide may compriseadministering to a mouse an amount of an immunogenic compositioncomprising the antigen polypeptide effective to stimulate a detectableimmune response, obtaining antibody-producing cells (e.g., cells fromthe spleen) from the mouse and fusing the antibody-producing cells withmyeloma cells to obtain antibody-producing hybridomas, and testing theantibody-producing hybridomas to identify a hybridoma that produces amonoclonal antibody that binds specifically to the antigen. Onceobtained, a hybridoma can be propagated in a cell culture, optionally inculture conditions where the hybridoma-derived cells produce themonoclonal antibody that binds specifically to the antigen. Themonoclonal antibody may be purified from the cell culture.

The adjective “specifically reactive with” as used in reference to anantibody is intended to mean, as is generally understood in the art,that the antibody is sufficiently selective between the antigen ofinterest (e.g., an ActRII receptor polypeptide) and other antigens thatare not of interest that the antibody is useful for, at minimum,detecting the presence of the antigen of interest in a particular typeof biological sample. In certain methods employing the antibody, such astherapeutic applications, a higher degree of specificity in binding maybe desirable. Monoclonal antibodies generally have a greater tendency(as compared to polyclonal antibodies) to discriminate effectivelybetween the desired antigens and cross-reacting polypeptides. Onecharacteristic that influences the specificity of an antibody:antigcninteraction is the affinity of the antibody for the antigen. Althoughthe desired specificity may be reached with a range of differentaffinities, generally preferred antibodies will have an affinity (adissociation constant) of about 10-6, 10-7, 10-8, 10-9 or less. Giventhe extraordinarily tight binding between activin and an ActRIIreceptor, it is expected that a neutralizing anti-activin or anti-ActRIIreceptor antibody would generally have a dissociation constant of 10-10or less.

In addition, the techniques used to screen antibodies in order toidentify a desirable antibody may influence the properties of theantibody obtained. For example, if an antibody is to be used for bindingan antigen in solution, it may be desirable to test solution binding. Avariety of different techniques are available for testing interactionbetween antibodies and antigens to identify particularly desirableantibodies. Such techniques include ELISAs, surface plasmon resonancebinding assays (e.g., the Biacore.TM. binding assay, Biacore AB,Uppsala, Sweden), sandwich assays (e.g., the paramagnetic bead system ofIGEN International, Inc., Gaithersburg, Md.), Western blots,immunoprecipitation assays, and immunohistochemistry.

In certain embodiments, ActRII receptor inhibitors to be used in thecompositions and methods described herein include alternative forms ofactivin, particularly those with alterations in the type I receptorbinding domain can bind to type II receptors and fail to form an activeternary complex. In certain embodiments, nucleic acids, such asantisense molecules, siRNAs or ribozymes that inhibit activin A, B, C orE, or, particularly, ActRII receptor expression, can be used in thecompositions and methods described herein.

In other embodiments, the inhibitors of ActRII receptors used in thecompositions and methods described herein are non-antibody proteins withActRII receptor antagonist activity, including inhibin (i.e., inhibinalpha subunit), follistatin (e.g., follistatin-288 and follistatin-315),Cerberus, follistatin related protein (“FSRP”), cndoglin, activin C,alpha(2)-macroglobulin, and an M108A (methionine to alanine change atposition 108) mutant activin A.

In a specific embodiment, the ActRII receptor inhibitor to be used inthe compositions and methods described herein is a follistatinpolypeptide that antagonizes activin bioactivity and/or binds toactivin. The term “follistatin polypeptide” includes polypeptidescomprising any naturally occurring polypeptide of follistatin as well asany variants thereof (including mutants, fragments, fusions, andpeptidomimetic forms) that retain a useful activity, and furtherincludes any functional monomer or multimer of follistatin. Variants offollistatin polypeptides that retain activin binding properties can beidentified based on previous studies involving follistatin and activininteractions. For example, WO2008/030367, which is included by referenceherein in its entirety, discloses specific follistatin domains (“FSDs”)that are shown to be important for activin binding. Follistatinpolypeptides include polypeptides derived from the sequence of any knownfollistatin having a sequence at least about 80% identical to thesequence of a follistatin polypeptide, and optionally at least 85%, 90%,95%, 96%, 97%, 98%, 99% or greater identity. Examples of follistatinpolypeptides include the mature follistatin polypeptide or shorterisoforms or other variants of the human follistatin precursorpolypeptide as described, for example, in WO2005/025601, which isincluded by reference herein in its entirety.

In a specific embodiment, the ActRII receptor inhibitor to be used inthe compositions and methods described herein is a follistatin-likerelated gene (FLRG) that antagonizes activin bioactivity and/or binds toactivin. The term “FLRG polypeptide” includes polypeptides comprisingany naturally occurring polypeptide of FLRG as well as any variantsthereof (including mutants, fragments, fusions, and peptidomimeticforms) that retain a useful activity. Variants of FLRG polypeptides thatretain activin binding properties can be identified using routinemethods to assay FLRG and activin interactions. See, for example, U.S.Pat. No. 6,537,966, which is included by reference herein in itsentirety. FLRG polypeptides include polypeptides derived from thesequence of any known FLRG having a sequence at least about 80%identical to the sequence of an FLRG polypeptide, and optionally atleast 85%, 90%, 95%, 96%, 97%, 98%, 99% or greater identity.

In certain embodiments, functional variants or modified forms of thefollistatin polypeptides and FLRG polypeptides include fusion proteinshaving at least a portion of the follistatin polypeptides or FLRGpolypeptides and one or more fusion domains, such as, for example,domains that facilitate isolation, detection, stabilization ormultimcrization of the polypeptide. Suitable fusion domains arediscussed in detail above with reference to the ActRIIA and ActRIIBpolypeptides. In one embodiment, an ActRII receptor inhibitor is afusion protein comprising an activin binding portion of a follistatinpolypeptide fused to an Fc domain. In another embodiment, an ActRIIreceptor inhibitor is a fusion protein comprising an activin bindingportion of an FLRG polypeptide fused to an Fc domain.

5.3 Assays (A) Diagnostic Assays (I) Bone Turnover

Various circulating markers of bone turnover can be used to diagnosebone disorders, such as low bone turnover. Circulating markers of boneturnover are markers of bone formation such as bone specific alkalinephosphatase (bAP), osteocalcin, procollagen type I C-terminal propeptide(PICP) and insulin-like growth factor-1 (IGF-1), some being markers ofbone resorption such as pyridinoline, deoxypyridinoline,tartrate-resistant acid phosphatase (TRAP), TRAP type 5b, pyridinoline,deoxypyridinoline and procollagen type I C-terminal telopeptide (ICTP),serum or urine collagen cross-links (N-telopeptide or C-telopeptide),and 25 hydroxyvitamin D. Assays to measure the entire parathyroidhormone (PTH) molecule can also be used. The skilled artisan is aware ofimaging methods allowing the assessment of bone mineral density (BMD).See, e.g., Tilman B. Drueke and Sharon M. Moe, Disturbances of bone andmineral metabolism in chronic kidney disease: an internationalinitiative to improve diagnosis and treatment, Nephrol Dial Transplant(2004) 19: 534-536; Okuno S, Inaba M., Biochemical markers of boneturnover. New aspect. Dialysis and bone metabolic marker, Clin Calcium.2009 Aug;19(8):1084-91; Herberth J, Monier-Faugere MC, Mawad HW,Branscum AJ, Herberth Z, Wang G, Cantor T, Malluche HH, The five mostcommonly used intact parathyroid hormone assays are useful for screeningbut not for diagnosing bone turnover abnormalities in CKD-5 patients,Clin Nephrol. 2009 Jul;72(1):5-14; Lehmann G, Ott U, Kaemmerer D,Schuetze J, Wolf G., Bone histomorphometry and biochemical markers ofbone turnover in patients with chronic kidney disease Stages 3-5, ClinNephrol. 2008 Oct;70(4):296-305; Drüeke TB., Is parathyroid hormonemeasurement useful for the diagnosis of renal bone disease?, Kidney Int.2008 Mar;73(6):674-6; Yamada S, Inaba M, Kurajoh M, Shidara K, ImanishiY, Ishimura E, Nishizawa Y., Utility of serum tartrate-resistant acidphosphatase (TRACP5b) as a bone resorption marker in patients withchronic kidney disease: independence from renal dysfunction., ClinEndocrinol (Oxf). 2008 Aug;69(2):189-96. Epub 2008 Jan 23. See also,Paul D. Miller, Diagnosis and Treatment of Osteoporosis in Chronic RenalDisease, 2009.

Another marker for monitoring bone resorption in CKD patients with mildrenal dysfunction is serum concentration of type I collagenN-telopeptide (S-NTX). See, e.g., Hamano T, Fujii N, Nagasawa Y, IsakaY, Moriyama T, Okada N, Imai E, Horio M, Ito T., Serum NTX is apractical marker for assessing antircsorptivc therapy for glucocorticoidtreated patients with chronic kidney disease., Bone. 2006Nov;39(5):1067-72. Epub 2006 Jun 16.

Quantitative computed tomography (QCT) can also be used to determinebone turnover.

(II) Adynamic Bone Disorder Model

A mouse model for adynamic bone disease in a renal setting is to use amouse nephrectomy model, such as the ⅚ nephrectomy model used inSections 6.2 and 6.3, wherein the mice are fed a low phosphate diet.

In another mouse model, mice are subjected to electrocautery of onekidney and nephrectomy of the other kidney. The mice are fedlow-phosphate chow supplemented with calcitriol. See, e.g., Lund et al.,2004, J Am Soc Nephrol 15:349-369.

(III) Tetracycline Labeling of Bone

A diagnostic test that can be used to determine the type of bone diseaseassociated with CKD is iliac crest bone biopsy with double tetracyclinelabeling and bone histomorphometric analysis. See, e.g., National KidneyFoundation: NKF KDOQI Guidelines.

(IV) Vascular Calcification

Non-contrast computed tomography (CT) for imaging the extent of coronaryartery calcification (CAC) and contrast CT for noninvasive coronaryangiography (CTA) are developments generally used to diagnoseobstructive coronary disease. Radionuclide stress testing, coronaryartery calcium scanning, and noninvasive coronary angiography fordiagnostic and prognostic cardiac assessment can also be used. See:Berman DS, Shaw LJ, Hachamovitch R, Friedman JD, Polk DM, Hayes SW,Thomson LE, Germano G, Wong ND, Kang X, Rozanski A., Comparative use ofradionuclide stress testing, coronary artery calcium scanning, andnoninvasive coronary angiography for diagnostic and prognostic cardiacassessment, Semin Nucl Med. 2007 Jan;37(1):2-16.

Coronary calcium screening results from asymptomatic patients can beused as a comparison. For example, calcium screening results obtainedprior to the onset of kidney disease can be used as a comparison whenvascular calcification is related to the kidney disease.

Possible methods of detecting and quantifying coronary arterycalcification (CAC) include, but arc not limited to, x-ray computedtomography and myocardial perfusion single photon emission computedtomography (SPECT). Moser KW, O′Keefe JH Jr, Bateman TM, McGhie IA.,Coronary calcium screening in asymptomatic patients as a guide to riskfactor modification and stress myocardial perfusion imaging, J NuclCardiol. 2003 Nov-Dec;1 0(6):590-8. Multi-detector computed tomography(MDCT) also can be used to detect vascular calcification (see, e.g.,Burrill et al., 2007, Postgrad. Med. J. 83(985):698-704).

Another diagnostic method for vascular calcification is fluorine 18fluorodeoxyglucose (FDG) uptake in the thoracic aortic wall at combinedpositron emission tomography (PET)/computed tomography (CT). See:Tatsumi M, Cohade C, Nakamoto Y, Wahl RL., Fluorodeoxyglucose uptake inthe aortic wall at PET/CT: possible finding for active atherosclerosis,Radiology. 2003 Dec;229(3):831-7. Epub 2003 Oct 30.

In even another embodiment, ultrafast CT can be used to detect thepresence of atherosclerotic coronary disease. See, e.g., Breen JF,Sheedy PF 2nd, Schwartz RS, Stanson AW, Kaufmann RB, Moll PP, RumbergerJA, Coronary artery calcification detected with ultrafast CT as anindication of coronary artery disease, Radiology. 1992 Nov;185(2):435-9.

Electron-beam computed tomography scanning can also be used to diagnosecoronary artery disease. See: Schmermund A, Baumgart D, Sack S,Möhlenkamp S, Grönemeyer D, Seibel R, Erbel R., Assessment of coronarycalcification by electron-beam computed tomography in symptomaticpatients with normal, abnormal or equivocal exercise stress test, EurHeart J. 2000 Oct;21(20):1674-82.

Another test for vascular calcification regards the plaque compositionin plexogenic and thromboembolic pulmonary hypertension. Chronicthromboembolic pulmonary hypertension is associated with atheroscleroticplaques with glycophorin-rich pultaceous cores, and plexogenic pulmonaryhypertension with fibrous plaques. Thromboembolic material plays acritical role in the formation of pultaceous cores, of which erythrocytemembrane derived glycophorin is a major component. Thereby, chronicthromboembolic and plexogenic pulmonary hypertension (primary andsecondary (Eisenmenger syndrome)) are investigated. See: Arbustini E,Morbini P, D′Armini AM, Repetto A, Minzioni G, Piovella F, Viganó M,Tavazzi L, Plaque composition in plexogenic and thromboembolic pulmonaryhypertension: the critical role of thrombotic material in pultaceouscore formation, Heart. 2002 Aug;88(2):177-82.

Agatston scoring, a calcium scoring system based on density measurementsof deposited calcium plaques, can be used to quantify vascularcalcification. In this system, levels of vascular calcification can bemeasured by multi-detector computed tomography (MDCT) and attenuationsin the rate of progression in the Agatston score can be assessed (see,e.g., Sharma et al., 2010, Vase. Health Risk Manag. 6:603-611).

Further, vascular calcification can be assessed using the methodsdescribed in Adragao et al., 2004, Nephrol. Dial. Transplant19:1480-1488.

Another assay for use in quantifying vascular calcification in a subjectis the lesion-specific calcium score, which comprises a method ofcalcium measurement that results from a CT test for coronary arterycalcification. This method is described by, e.g., Akram and Voros, 2008,Int. J. cardiovac. Imaging 14:743-749.

(V) Kidney Disease

Glomerular filtration rate can be determined by any method known to theskilled artisan to determine kidney disease. See website of the NationalKidney Foundation.

(VI) Secondary Parathyroidism

Secondary hyperparathyroidism occurs when the parathyroid glands producetoo much parathyroid hormone (PTH) because of too low calcium levels orincreased phosphorus levels. Calcium, phosphorus, and PTH levels can bedetermined from blood samples.

(VII) Hyperphosphatemia

Abnormally elevated levels of phosphate in the blood can be determinedby any method known to the skilled artisan.

(B) Screening Assays

Various ActRII polypeptide variants, or soluble ActRII polypeptidevariants, may be tested for their ability to inhibit ActRII. Inaddition, compounds can be tested for their ability to inhibit ActRII.Once inhibitors of ActRII activity are confirmed, these compounds can beused with the methods provided herein. ActRII can be ActRIIA or ActRIIB.The assays below are described for ActRIIA but can be performedanalogously for ActRIIB.

For example, the effect of an ActRIIA polypeptide variant on theexpression of genes involved in bone production or bone destruction maybe assessed. This may, as needed, be performed in the presence of one ormore recombinant ActRIIA ligand proteins (e.g., activin), and cells maybe transfected so as to produce an ActRIIA polypeptide and/or variantsthereof, and optionally, an ActRIIA ligand. Likewise, an ActRIIApolypeptide may be administered to a mouse or other animal, and one ormore bone properties, such as density or volume may be assessed. Thehealing rate for bone fractures may also be evaluated. Dual-energy x-rayabsorptiometry (DEXA) is a well-established, non-invasive, quantitativetechnique for assessing bone density in an animal. In humans centralDEXA systems may be used to evaluate bone density in the spine andpelvis. These are the best predictors of overall bone density.Peripheral DEXA systems may be used to evaluate bone density inperipheral bones, including, for example, the bones of the hand, wrist,ankle and foot. Traditional x-ray imaging systems, including CAT scans,may be used to evaluate bone growth and fracture healing. In addition,bone density can be measured using quantitative computed tomography(qCT). The mechanical strength of bone may also be evaluated.

In certain aspects, provided herein is the use of ActRIIA polypeptides(e.g., soluble ActRIIA polypeptides) and activin polypeptides toidentify compounds (agents) which are agonist or antagonists of theactivin-ActRIIA signaling pathway. Compounds identified through thisscreening can be tested to assess their ability to modulate bone growthor mineralization in vitro. Optionally, these compounds can further betested in animal models to assess their ability to modulate tissuegrowth in vivo.

There arc numerous approaches to screening for therapeutic agents formodulating tissue growth by targeting activin and ActRIIA polypeptides.In certain embodiments, high-throughput screening of compounds can becarried out to identify agents that perturb activin or ActRIIA-mediatedeffects on bone. In certain embodiments, the assay is carried out toscreen and identify compounds that specifically inhibit or reducebinding of an ActRIIA polypeptide to activin. Alternatively, the assaycan be used to identify compounds that enhance binding of an ActRIIApolypeptide to activin. In a further embodiment, the compounds can beidentified by their ability to interact with an activin or ActRIIApolypeptide.

A variety of assay formats will suffice and, in light of the presentdisclosure, those not expressly described herein will nevertheless becomprehended by one of ordinary skill in the art. As described herein,the test compounds (agents) used herein may be created by anycombinatorial chemical method. Alternatively, the subject compounds maybe naturally occurring biomolecules synthesized in vivo or in vitro.Compounds (agents) to be tested for their ability to act as modulatorsof tissue growth can be produced, for example, by bacteria, yeast,plants or other organisms (e.g., natural products), produced chemically(e.g., small molecules, including peptidomimetics), or producedrecombinantly. Test compounds contemplated herein include non-peptidylorganic molecules, peptides, polypeptides, peptidomimetics, sugars,hormones, and nucleic acid molecules. In a specific embodiment, the testagent is a small organic molecule having a molecular weight of less thanabout 2,000 daltons.

The test compounds can be provided as single, discrete entities, orprovided in libraries of greater complexity, such as made bycombinatorial chemistry. These libraries can comprise, for example,alcohols, alkyl halides, amines, amides, esters, aldehydes, ethers andother classes of organic compounds. Presentation of test compounds tothe test system can be in either an isolated form or as mixtures ofcompounds, especially in initial screening steps. Optionally, thecompounds may be derivatized with other compounds and have derivatizinggroups that facilitate isolation of the compounds. Non-limiting examplesof derivatizing groups include biotin, fluorescein, digoxygenin, greenfluorescent protein, isotopes, polyhistidine, magnetic beads,glutathione S transferase (GST), photoactivatible crosslinkers or anycombinations thereof.

In many drug screening programs which test libraries of compounds andnatural extracts, high throughput assays are desirable in order tomaximize the number of compounds surveyed in a given period of time.Assays which are performed in cell-free systems, such as may be derivedwith purified or semi-purified proteins, are often preferred as“primary” screens in that they can be generated to permit rapiddevelopment and relatively easy detection of an alteration in amolecular target which is mediated by a test compound. Moreover, theeffects of cellular toxicity or bioavailability of the test compound canbe generally ignored in the in vitro system, the assay instead beingfocused primarily on the effect of the drug on the molecular target asmay be manifest in an alteration of binding affinity between an ActRIIApolypeptide and activin.

Merely to illustrate, in an exemplary screening assay, the compound ofinterest is contacted with an isolated and purified ActRIIA polypeptidewhich is ordinarily capable of binding to activin. To the mixture of thecompound and ActRIIA polypeptide is then added a composition containingan ActRIIA ligand. Detection and quantification of ActRIIA/activincomplexes provides a means for determining the compound’s efficacy atinhibiting (or potentiating) complex formation between the ActRIIApolypeptide and activin. The efficacy of the compound can be assessed bygenerating dose response curves from data obtained using variousconcentrations of the test compound. Moreover, a control assay can alsobe performed to provide a baseline for comparison. For example, in acontrol assay, isolated and purified activin is added to a compositioncontaining the ActRIIA polypeptide, and the formation of ActRIIA/activincomplex is quantitated in the absence of the test compound. It will beunderstood that, in general, the order in which the reactants may beadmixed can be varied, and can be admixed simultaneously. Moreover, inplace of purified proteins, cellular extracts and lysates may be used torender a suitable cell-free assay system.

Complex formation between the ActRIIA polypeptide and activin may bedetected by a variety of techniques. For instance, modulation of theformation of complexes can be quantitated using, for example, detectablylabeled proteins such as radiolabeled (e.g., ³²P, ³⁵S, ¹⁴C or ³H),fluorescently labeled (e.g., FITC), or enzymatically labeled ActRIIApolypeptide or activin, by immunoassay, or by chromatographic detection.

In certain embodiments, contemplated herein is the use of fluorescencepolarization assays and fluorescence resonance energy transfer (FRET)assays in measuring, either directly or indirectly, the degree ofinteraction between an ActRIIA polypeptide and its binding protein.Further, other modes of detection, such as those based on opticalwaveguides (PCT Publication WO 96/26432 and U.S. Pat. No. 5,677,196),surface plasmon resonance (SPR), surface charge sensors, and surfaceforce sensors, are compatible with many embodiments described herein.

Moreover, an interaction trap assay, also known as the “two hybridassay,” can be used for identifying agents that disrupt or potentiateinteraction between an ActRIIA polypeptide and its binding protein. Seefor example, U.S. Pat. No. 5,283,317; Zervos et al. (1993) Cell72:223-232; Madura et al. (1993) J Biol Chem 268:12046-12054; Bartel etal. (1993) Biotechniques 14:920-924; and Iwabuchi et al. (1993) Oncogene8:1693-1696). In a specific embodiment, contemplated herein is the useof reverse two hybrid systems to identify compounds (e.g., smallmolecules or peptides) that dissociate interactions between an ActRIIApolypeptide and its binding protein. See for example, Vidal and Legrain,(1999) Nucleic Acids Res 27:919-29; Vidal and Legrain, (1999) TrendsBiotechnol 17:374-81; and U.S. Pat. Nos. 5,525,490; 5,955,280; and5,965,368.

In certain embodiments, the subject compounds are identified by theirability to interact with an ActRIIA or activin polypeptide. Theinteraction between the compound and the ActRIIA or activin polypeptidemay be covalent or non-covalent. For example, such interaction can beidentified at the protein level using in vitro biochemical methods,including photo-crosslinking, radiolabeled ligand binding, and affinitychromatography (Jakoby W B et al., 1974, Methods in Enzymology 46: 1).In certain cases, the compounds may be screened in a mechanism basedassay, such as an assay to detect compounds which bind to an activin orActRIIA polypeptide. This may include a solid phase or fluid phasebinding event. Alternatively, the gene encoding an activin or ActRIIApolypeptide can be transfected with a reporter system (e.g.,β-galactosidase, luciferase, or green fluorescent protein) into a celland screened against the library preferably by a high throughputscreening or with individual members of the library. Other mechanismbased binding assays may be used, for example, binding assays whichdetect changes in free energy. Binding assays can be performed with thetarget fixed to a well, bead or chip or captured by an immobilizedantibody or resolved by capillary electrophoresis. The bound compoundsmay be detected usually using colorimetric or fluorescence or surfaceplasmon resonance.

In certain aspects, provided herein are methods and agents formodulating (stimulating or inhibiting) bone formation and increasingbone mass. Therefore, any compound identified can be tested in wholecells or tissues, in vitro or in vivo, to confirm their ability tomodulate bone growth or mineralization. Various methods known in the artcan be utilized for this purpose. In particular, the compounds can betested for their ability to increase bone turnover.

For example, the effect of the ActRIIA or activin polypeptides or testcompounds on bone or cartilage growth can be determined by measuringinduction of Msx2 or differentiation of osteoprogenitor cells intoosteoblasts in cell based assays (see, e.g., Daluiski et al., Nat Genet.2001, 27(1):84-8; Hino et al., Front Biosci. 2004, 9:1520-9). Anotherexample of cell-based assays includes analyzing the osteogenic activityof the subject ActRIIA or activin polypeptides and test compounds inmesenchymal progenitor and osteoblastic cells. To illustrate,recombinant adenoviruses expressing an activin or ActRIIA polypeptidecan be constructed to infect pluripotent mesenchymal progenitorC3H10T1/2 cells, preosteoblastic C2C12 cells, and osteoblastic TE-85cells. Osteogenic activity is then determined by measuring the inductionof alkaline phosphatase, osteocalcin, and matrix mineralization (see,e.g., Cheng et al., J bone Joint Surg Am. 2003, 85-A(8): 1544-52).

Also provided herein are in vivo assays to measure bone or cartilagegrowth. For example, Namkung-Matthai et al., Bone, 28:80-86 (2001)discloses a rat osteoporotic model in which bone repair during the earlyperiod after fracture is studied. Kubo et al., Steroid Biochemistry &Molecular Biology, 68:197-202 (1999) also discloses a rat osteoporoticmodel in which bone repair during the late period after fracture isstudied. Andersson et al., J. Endocrinol. 170:529-537 describe a mouseosteoporosis model in which mice are ovariectomized, which causes themice to lose substantial bone mineral content and bone mineral density,with the trabecular bone losing roughly 50% of bone mineral density.Bone density could be increased in the ovariectomized mice byadministration of factors such as parathyroid hormone. In certainaspects, fracture healing assays that are known in the art can be used.These assays include fracture technique, histological analysis, andbiomechanical analysis, which are described in, for example, U.S. Pat.No. 6,521,750, which is incorporated by reference in its entirety forits disclosure of experimental protocols for causing as well asmeasuring the extent of fractures, and the repair process.

5.4 Dose

Provided herein are methods for the treatment of CKD-MBD and / or lowturnover bone disease, wherein the methods comprise administering to apatient in need of treatment a therapeutically effective amount of aninhibitor of ActRII (see Section 5.2). In certain embodiments, an ActRIIinhibitor is an inhibitor of ActRIIA as set forth in Section 5.2(a). Inother embodiments, an ActRII inhibitor is an inhibitor of ActRIIB as setforth in Section 5.2(b). In certain embodiments, an ActRII inhibitor isa combination of an ActRIIA inhibitor and an ActRIIB inhibitor.

In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor is sufficient to ameliorate one symptom of CKD-MBD. In certainembodiments, a therapeutically effective amount of an ActRII inhibitoris sufficient to prevent at least one symptom of CKD-MBD from worsening.

In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor improves or stabilizes kidney function. Kidney function can bemeasured by glomerular filtration rate. See, e.g., Section 5.4(a)(iv).In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor is a daily dose that is sufficient to stabilize the glomerularfiltration rate of a CKD-MBD patient for the duration of treatment withActRII inhibitor and for at least 3 months, 6 months, 9 months, or 12months. In certain embodiments, a therapeutically effective amount of anActRIIA inhibitor is a daily dose that is sufficient to increase theglomerular filtration rate by at least 5%, 10%, 15%, 20%, 25%, 30%, 35%,40%, 45%, or at least 50%.

In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor increases the red blood cell level and / or hemoglobin levelsin the patient.

In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor is effective to (a) increase red blood cell and / orhemoglobin levels in the patient; (b) improvement in bone quality and /or bone mineral density in the patient; and (c) improve kidney functionin the patient.

In certain embodiments, a therapeutically effective amount of an ActRIIinhibitor is effective to (a) increase red blood cell and / orhemoglobin levels in the patient; (b) increase the bone turnover in thepatient; and (c) improve kidney function in the patient.

In certain embodiments, the ActRII inhibitor is dosed at intervals andamounts sufficient to achieve serum concentrations of 0.2 microgram/kgor greater, and serum levels of 1 microgram/kg or 2 microgram/kg orgreater are desirable for achieving significant effects on bone densityand strength. Dosing regimens may be designed to reach serumconcentrations of between 0.2 and 15 microgram/kg, and optionallybetween 1 and 5 microgram/kg. In humans, serum levels of 0.2microgram/kg may be achieved with a single dose of 0.1 mg/kg or greaterand serum levels of 1 microgram/kg may be achieved with a single dose of0.3 mg/kg or greater. The observed serum half-life of the molecule isbetween about 20 and 30 days, substantially longer than most Fc fusionproteins, and thus a sustained effective serum level may be achieved,for example, by dosing with 0.2-0.4 mg/kg on a weekly or biweekly basis,or higher doses may be used with longer intervals between dosings. Forexample, doses of 1-3 mg/kg might be used on a monthly or bimonthlybasis, and the effect on bone may be sufficiently durable that dosing isnecessary only once every 3, 4, 5, 6, 9, 12 or more months.

5.5 Pharmaceutical Compositions

In certain embodiments, activin-ActRII antagonists (e.g., ActRIIpolypeptides) are formulated with a pharmaceutically acceptable carrierfor use with the methods described herein. For example, an ActRIIpolypeptide can be administered alone or as a component of apharmaceutical formulation (therapeutic composition). The subjectcompounds may be formulated for administration in any convenient way foruse in human or veterinary medicine. ActRII can be ActRIIA or ActRIIB.

In certain embodiments, the therapeutic methods described herein includeadministering the composition systemically, or locally as an implant ordevice. When administered, the therapeutic compositions used herein canbe in a pyrogen-free, physiologically acceptable form. Therapeuticallyuseful agents other than the ActRIIA antagonists which may alsooptionally be included in the composition as described above, may beadministered simultaneously or sequentially with the subject compounds(e.g., ActRII polypeptides, such as ActRIIA and / or ActRIIBpolypeptides (see Section 5.2)).

Typically, ActRIIA antagonists will be administered parenterally.Pharmaceutical compositions suitable for parenteral administration maycomprise one or more ActRII polypeptides in combination with one or morepharmaceutically acceptable sterile isotonic aqueous or nonaqueoussolutions, dispersions, suspensions or emulsions, or sterile powderswhich may be reconstituted into sterile injectable solutions ordispersions just prior to use, which may contain antioxidants, buffers,bacteriostats, solutes which render the formulation isotonic with theblood of the intended recipient or suspending or thickening agents.Examples of suitable aqueous and nonaqueous carriers which may beemployed in the pharmaceutical compositions used in the methodsdescribed herein include water, ethanol, polyols (such as glycerol,propylene glycol, polyethylene glycol, and the like), and suitablemixtures thereof, vegetable oils, such as olive oil, and injectableorganic esters, such as ethyl oleate. Proper fluidity can be maintained,for example, by the use of coating materials, such as lecithin, by themaintenance of the required particle size in the case of dispersions,and by the use of surfactants.

Further, the composition may be encapsulated or injected in a form fordelivery to a target tissue site (e.g., bone). In certain embodiments,compositions used in the methods described herein may include a matrixcapable of delivering one or more therapeutic compounds (e.g., ActRIIApolypeptides) to a target tissue site (e.g., bone), providing astructure for the developing tissue and optimally capable of beingresorbed into the body. For example, the matrix may provide slow releaseof the ActRIIA polypeptides. Such matrices may be formed of materialspresently in use for other implanted medical applications.

The choice of matrix material is based on biocompatibility,biodegradability, mechanical properties, cosmetic appearance andinterface properties. The particular application of the subjectcompositions will define the appropriate formulation. Potential matricesfor the compositions may be biodegradable and chemically defined calciumsulfate, tricalciumphosphate, hydroxyapatite, polylactic acid andpolyanhydrides. Other potential materials are biodegradable andbiologically well defined, such as bone or dermal collagen. Furthermatrices are comprised of pure proteins or extracellular matrixcomponents. Other potential matrices are non-biodegradable andchemically defined, such as sintered hydroxyapatite, bioglass,aluminates, or other ceramics. Matrices may be comprised of combinationsof any of the above mentioned types of material, such as polylactic acidand hydroxyapatite or collagen and tricalciumphosphate. The bioceramicsmay be altered in composition, such as in calcium-aluminate-phosphateand processing to alter pore size, particle size, particle shape, andbiodegradability.

In certain embodiments, the compositions used in the methods describedherein can be administered orally, e.g., in the form of capsules,cachets, pills, tablets, lozenges (using a flavored basis, usuallysucrose and acacia or tragacanth), powders, granules, or as a solutionor a suspension in an aqueous or non-aqueous liquid, or as anoil-in-water or water-in-oil liquid emulsion, or as an elixir or syrup,or as pastilles (using an inert base, such as gelatin and glycerin, orsucrose and acacia) and/or as mouth washes and the like, each containinga predetermined amount of an agent as an active ingredient. An agent mayalso be administered as a bolus, electuary or paste.

In solid dosage forms for oral administration (capsules, tablets, pills,dragees, powders, granules, and the like), one or more therapeuticcompounds used in the methods described herein may be mixed with one ormore pharmaceutically acceptable carriers, such as sodium citrate ordicalcium phosphate, and/or any of the following: (1) fillers orextenders, such as starches, lactose, sucrose, glucose, mannitol, and/orsilicic acid; (2) binders, such as, for example, carboxymethylcellulose,alginates, gelatin, polyvinyl pyrrolidone, sucrose, and/or acacia; (3)humectants, such as glycerol; (4) disintegrating agents, such asagar-agar, calcium carbonate, potato or tapioca starch, alginic acid,certain silicates, and sodium carbonate; (5) solution retarding agents,such as paraffin; (6) absorption accelerators, such as quaternaryammonium compounds; (7) wetting agents, such as, for example, cetylalcohol and glycerol monostearate; (8) absorbents, such as kaolin andbentonite clay; (9) lubricants, such as talc, calcium stearate,magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate,and mixtures thereof; and (10) coloring agents. In the case of capsules,tablets and pills, the pharmaceutical compositions may also comprisebuffering agents. Solid compositions of a similar type may also beemployed as fillers in soft and hard-filled gelatin capsules using suchexcipients as lactose or milk sugars, as well as high molecular weightpolyethylene glycols and the like.

Liquid dosage forms for oral administration include pharmaceuticallyacceptable emulsions, microemulsions, solutions, suspensions, syrups,and elixirs. In addition to the active ingredient, the liquid dosageforms may contain inert diluents commonly used in the art, such as wateror other solvents, solubilizing agents and emulsifiers, such as ethylalcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzylalcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils(in particular, cottonseed, groundnut, corn, germ, olive, castor, andsesame oils), glycerol, tetrahydrofuryl alcohol, polyethylene glycolsand fatty acid esters of sorbitan, and mixtures thereof. Besides inertdiluents, the oral compositions can also include adjuvants such aswetting agents, emulsifying and suspending agents, sweetening,flavoring, coloring, perfuming, and preservative agents.

Suspensions, in addition to the active compounds, may contain suspendingagents such as ethoxylated isostearyl alcohols, polyoxyethylenesorbitol, and sorbitan esters, microcrystalline cellulose, aluminummetahydroxide, bentonite, agar-agar and tragacanth, and mixturesthereof.

The compositions used in the methods described herein may also containadjuvants, such as preservatives, wetting agents, emulsifying agents anddispersing agents. Prevention of the action of microorganisms may beensured by the inclusion of various antibacterial and antifungal agents,for example, paraben, chlorobutanol, phenol sorbic acid, and the like.It may also be desirable to include isotonic agents, such as sugars,sodium chloride, and the like into the compositions. In addition,prolonged absorption of the injectable pharmaceutical form may bebrought about by the inclusion of agents which delay absorption, such asaluminum monostearate and gelatin.

It is understood that the dosage regimen will be determined by theattending physician considering various factors which modify the actionof the subject compounds used in the methods described herein (e.g.,ActRII polypeptides, such as ActRIIA and / or ActRIIB polypeptides (seeSection 5.2)). The various factors include, but are not limited to,amount of bone weight desired to be formed, the degree of bone densityloss, the site of bone damage, the condition of the damaged bone, thepatient’s age, sex, and diet, the severity of any disease that may becontributing to bone loss, time of administration, and other clinicalfactors. Optionally, the dosage may vary with the type of matrix used inthe reconstitution and the types of compounds in the composition. Theaddition of other known growth factors to the final composition, mayalso affect the dosage. Progress can be monitored by periodic assessmentof bone growth and/or repair, for example, X-rays (including DEXA),histomorphometric determinations, and tetracycline labeling.

In certain embodiments, the methods described herein comprise genetherapy for the in vivo production of ActRII polypeptides. Such therapywould achieve its therapeutic effect by introduction of the ActRIIpolynucleotide sequences into cells or tissues having the disorders aslisted above. Delivery of ActRII polynucleotide sequences can beachieved using a recombinant expression vector such as a chimeric virusor a colloidal dispersion system. Preferred for therapeutic delivery ofActRII polynucleotide sequences is the use of targeted liposomes. TheActRII polypeptides can be ActRIIA and / or ActRIIB polypeptides (seeSection 5.2)).

Various viral vectors which can be utilized for gene therapy as taughtherein include adenovirus, herpes virus, vaccinia, or, preferably, anRNA virus such as a retrovirus. Preferably, the retroviral vector is aderivative of a murine or avian retrovirus. Examples of retroviralvectors in which a single foreign gene can be inserted include, but arenot limited to: Moloney murine leukemia virus (MoMuLV), Harvey murinesarcoma virus (HaMuSV), murine mammary tumor virus (MuMTV), and RousSarcoma Virus (RSV). A number of additional retroviral vectors canincorporate multiple genes. All of these vectors can transfer orincorporate a gene for a selectable marker so that transduced cells canbe identified and generated. Retroviral vectors can be madetarget-specific by attaching, for example, a sugar, a glycolipid, or aprotein. Preferred targeting is accomplished by using an antibody. Thoseof skill in the art will recognize that specific polynucleotidesequences can be inserted into the retroviral genome or attached to aviral envelope to allow target specific delivery of the retroviralvector containing the ActRIIA polynucleotide. In a preferred embodiment,the vector is targeted to bone or cartilage.

Alternatively, tissue culture cells can be directly transfected withplasmids encoding the retroviral structural genes gag, pol and env, byconventional calcium phosphate transfection. These cells are thentransfected with the vector plasmid containing the genes of interest.The resulting cells release the retroviral vector into the culturemedium.

Another targeted delivery system for ActRIIA polynucleotides is acolloidal dispersion system. Colloidal dispersion systems includemacromolecule complexes, nanocapsules, microspheres, beads, andlipid-based systems including oil-in-water emulsions, micelles, mixedmicelles, and liposomes. One colloidal system that can be used is aliposome. Liposomes are artificial membrane vesicles which are useful asdelivery vehicles in vitro and in vivo. RNA, DNA and intact virions canbe encapsulated within the aqueous interior and be delivered to cells ina biologically active form (see e.g., Fraley, et al., Trends Biochem.Sci., 6:77, 1981). Methods for efficient gene transfer using a liposomevehicle, are known in the art, see e.g., Mannino, et al., Biotechniques,6:682, 1988. The composition of the liposome is usually a combination ofphospholipids, usually in combination with steroids, especiallycholesterol. Other phospholipids or other lipids may also be used. Thephysical characteristics of liposomes depend on pH, ionic strength, andthe presence of divalent cations.

Examples of lipids useful in liposome production include phosphatidylcompounds, such as phosphatidylglyccrol, phosphatidylcholine,phosphatidylserine, phosphatidylethanolamine, sphingolipids,cerebrosides, and gangliosides. Illustrative phospholipids include eggphosphatidylcholine, dipalmitoylphosphatidylcholine, anddistearoylphosphatidylcholine. The targeting of liposomes is alsopossible based on, for example, organ-specificity, cell-specificity, andorganelle-specificity and is known in the art.

In certain embodiments, the ActRIIA inhibitor is substantially pure in apharmaceutical composition. Specifically, at most 20%, 10%, 5%, 2.5%,1%, 0.1%, or at most 0.05% of the compounds in the pharmaceuticalcomposition arc compounds other than the ActRII inhibitor and thepharmaceutical acceptable carrier.

EXAMPLES 6.1 Example 1 (A) ActRIIA-Fc Fusion Proteins

A soluble ActRIIA fusion protein that has the extracellular domain ofhuman ActRIIA fused to a human or mouse Fc domain with a minimal linkeris described. The constructs are referred to as ActRIIA-hFc andmActRIIA-Fc, respectively. ActRIIA-hFc is provided as SEQ ID NO:7.mActRIIA-Fc is the murine counterpart to SEQ ID NO:7.

The ActRIIA-hFc and mActRIIA-Fc proteins were expressed in CHO celllines. Three different leader sequences were considered:

-   (i) Honey bee mellitin (HBML): SEQ ID NO: 8-   (ii) Tissue Plasminogen Activator (TPA): SEQ ID NO: 9-   (iii) Native ActRIIA: SEQ ID NO: 10

The selected form employs the TPA leader and has the followingunprocessed amino acid sequence is set forth in SEQ ID NO: 13. Thispolypeptide is encoded by SEQ ID NO: 14.

(B) ActRIIB-Fc Fusion Proteins

Co-crystal structure of an extracellular domain of human ActRIIB fusedto a human Fc domain and Activin did not show any role for the final(C-terminal) 15 amino acids (referred to as the “tail” herein) of theextracellular domain in ligand binding. This sequence failed to resolveon the crystal structure, suggesting that these residues are present ina flexible loop that did not pack uniformly in the crystal. Thompson etal. EMBO J. 2003 Apr 1 ;22(7):1555-66. This sequence is also poorlyconserved between ActRIIB and ActRIIA. Accordingly, these residues wereomitted in the basic, or background, ActRIIB-Fc fusion construct.Additionally, position 64 in the background form is occupied by analanine, which is generally considered the “wild type” form, although aA64R allele occurs naturally. Thus, the background ActRIIB-Fc fusion hasthe sequence disclosed as SEQ ID NO:21.

Surprisingly, the C-terminal tail was found to enhance activin andGDF-11 binding, thus a preferred version of ActRIIB-Fc has a sequenceSEQ ID NO:20.

A variety of ActRIIB a variants that may be used according to themethods described herein are described in the International PatentApplication published as WO2006/012627 (see e.g., pp. 59-60),incorporated herein by reference in its entirety.

6.2 Effects of mACTRIIA Inhibition in a Mouse Model of Chronic KidneyDisease

This study was designed to study the effects of soluble mouse ActRIIAfused with mouse Fc via a minimal linker (SEQ ID NO:15) on treatment ofblood and bone parameters in a mouse model of chronic kidney disease andCKD-MBD.

Patients with chronic kidney disease (CKD) can become anemic and alsobecome osteopenic. Mice with partial renal ablation (⅚ nephrectomy) wereused as a model of CKD to test the effects of the polypeptide with theamino acid sequence of SEQ ID NO:15 in this model. Mice received twosurgeries to 1) remove one kidney completely and 2) to ligate 2 of the 3renal arteries in the remaining kidney. Sham operated mice were alsoincluded as controls. The sham or ⅚ nephrectomy surgeries were performedat Jackson Laboratories.

After mice were received they were placed on high fat diet for theduration of the study. Two weeks after the final surgery mice weredivided into groups (both SHAM and CKD) and began dosing with vehicle(PBS) or mActRIIA-Fc at 10 mg/kg twice per week for 8 weeks. Completeblood counts (CBC) were taken periodically during the study to assessfor anemia.

Bone mineral density was determined using dual energy x-rayabsorptiometry (DEXA, PIXIMus). At the conclusion of the studynecropsies were conducted to collect the long bones of the hind limbsand major organs. The remnant kidney was sent for histology processingand staining with H&E or Trichrome stain. Femurs were scanned by uCT(Scanco) to determine bone microarchitecture.

Mice appeared normal and healthy throughout the study period and put onweight as the study progressed (FIG. 1 ). Bone mineral density increasedin all four groups of mice, but mActRIIA-Fc treated mice (SHAM and CKD)had greater increases than either vehicle treated group (FIG. 2 ).mActRIIA-Fc treatment in CKD mice had bone mineral densities thatequaled or exceeded SHAM-VEH treated mice by the end of the study. CKDmice also became anemic by the end of the study (HCT < 40%), butmActRIIA-Fc treatment prevented anemia in the CKD group (HCT > 40%; FIG.3 ). mActRIIA-Fc treated mice in the SHAM group also showed increases inHCT when compared to VEH controls. Micro CT analysis of femurs afterdissection showed increases in trabecular bone in the mActRIIA-Fctreated mice, but there were no major differences between the SHAM andCKD vehicle treated groups at this time in the disease progression. Atnecropsy, no major differences in organ weights were observed, althoughmActRIIA-Fc treated mice had a slight increase in fat pad weights.Trichrome stained histological sections of the remnant kidney did notindicate widespread fibrosis at this point in the study in the CKD mice.

6.3 mACTRIIA Inhibition Prevents Anemia and Bone Loss in a TherapeuticModel of Established Kidney Disease

The ⅚ nephrectomy surgery in rodents is a commonly performedexperimental protocol used to model chronic kidney disease. In thistwo-phase surgery ⅔ of one kidney and the complete kidney on thecontralateral side are removed using aseptic surgical procedures. As aresult of the surgery the animal experiences impaired kidney functionand exhibits physiologic behavior analogous to humans with chronickidney disease.

Sham or ⅚ nephrectomy surgery was performed at Jackson Laboratoriesaccording to standard operating procedures. Animals were allowed torecover from surgery and then shipped. Animals were acclimated tolaboratory conditions for a minimum of 48 hours prior to the firstmeasurements being made. During this period all animals were observedfor any signs of clinical abnormalities that would exclude them fromstudy. Animals were assigned a study number on their cage cards anduniquely identified by ear notching.

ActRIIA-mIgG2aFc was diluted using Sterile PBS to a concentration of 2.0mg/ml. The dosing concentration: was 2.0 mg/ml. ActRIIA-mIgG2aFc wasstored at -65° C. ± 15° C., material may be thawed at room temperature,or overnight at 4° C. Thawed protein was kept on wet ice until use.

Thirty C57BL/6 female mice (10 weeks old) underwent a ⅚ nephrectomysurgery in which one kidney is completely removed followed by ligationof 2 of 3 renal veins ligated in the remaining kidney two weeks later.Sham surgeries were also performed on thirty C57BL/6 females in whichthe animals are subject to the same abdominal surgical procedure withoutremoval of the kidneys. After recovery from the second surgery animalswere shipped and allowed to acclimate to laboratory conditions for aminimum of 48 hours. Two months after the second surgery mice wererandomly assigned to one of four treatment groups with 15 mice per group(Table 2). Mice were weighed and dosed with either mActRIIA-Fc or PBStwice per week, for a total of 8 weeks. Longitudinal measurements ofbone mineral density (BMD) and hematological parameters were made atbaseline, an interim time point and at the conclusion of the study. Atnecropsy bones were collected and stored for histological examination orfor analysis by microCT scanning.

TABLE 2 Group N Mice Diet Treatment Surgery Concentration Route 1 15C57BL/6 Chow PBS Sham volume S.C. 2 15 C57BL/6 Chow mActRIIA-Fc Sham 10mg/kg S.C. 3 15 C57BL/6 Chow PBS ⅚ Nephr. volume S.C. 4 15 C57BL/6 ChowmActRIIA-Fc ⅚ Nephr. 10 mg/kg S.C.

(A) Experimental Procedures (I) Surgical Modification

Female C57BL/6 mice aged 10 weeks were given a two-stage surgery toaccomplish a ⅚ nephrectomy or the equivalent sham surgery.

(II) Animal Dosing

Dosing in the current study commenced one month after the completion ofthe ⅚ nephrectomy surgery. Mice were weighed and administered either PBSor mActRIIA-Fc at 10 mg/kg twice per week by subcutaneous injection.

(III) DXA Scanning

Longitudinal measurements of BMD were made monthly on anesthetized miceusing DXA scanning (Lunar PIXIMus, GE Medical Systems). During DXA scananalysis of BMD the mouse head was eliminated from the region ofinterest prevent quantification artifacts associated with the skull.

(IV) Blood Collection

Longitudinal measurements of complete blood counts (HM2, VetScan) weremade on blood collected by monthly submandibular bleeding. At thetermination of the study a terminal bleed was performed, blood wascollected and divided into either an EDTA containing tube for CBCanalysis or into a serum separation tube for serum collection. Serum wasfrozen at -80° for future analyses.

(V) Serum Analyses

Frozen serum was defrosted and 100 microliter were analyzed using aVetscan VS2 analyzer (Abaxis, Inc.). A comprehensive diagnostic rotorwas used to analyzes the samples for serum albumin (ALB), alkalinephosphatase (ALP), alanine aminotransferase (ALT), amylase (AMY), totalbilirubin (TBIL), blood urea nitrogen (BUN), total calcium (Ca++),Phosphorus (PHOS), creatinine (CRE), glucose (GLU), sodium (NA+),potassium (K+), total protein (TP) and globulin (GLOB).

(VI) Necropsy

At the conclusion of the study mice were euthanized by CO₂ inhalation.The kidneys and spleens were removed, weighed and stored in 10%formalin. The tibiae and femurs were collected and stored in 70%ethanol.

(VII) microCT Analysis

At the termination of the experiment the left femur and tibia from eachmouse were dissected and fixed in 70% ethanol. Bones were scanned usinga Scanco microCT (VivaCT75, Scanco) at 55 kV, 145 microA and a voxelsize of 20 microm. Scanned images were reconstructed using theincorporated Scanco software. Trabecular bone volume (BV/TV) andtrabecular thickness (Tb.Th) were assessed in a 400 microm section ofbone which was positioned 200 microm from the distal tip of the femur.Cortical thickness was measured in a 200 microm section of bone centeredat the mid-line of the femur.

(VIII) Data Analysts

Comparisons between mActRIIA-Fc and vehicle treated mice and tissueswere performed by Student’s t-Test using Microsoft Excel. Data areexpressed as mean ± SEM.

(B) Results

We investigated the ability of mActRIIA-Fc to prevent anemia and boneloss in a mouse model of chronic kidney disease. After 2 months ofdisease progression following the ⅚ nephrectomy surgery (Day 0), ⅚nephrectomized mice (CKD) exhibited a significant decrease in hematocritcompared to the sham cohorts (-5.4%, P<0.01). Longitudinal bloodsampling and subsequent CBC analysis showed that mActRIIA-Fc treatedmice in both the CKD and sham cohorts displayed significant increases inhematocrit compared to their VEH treated counterparts after 4 and 8weeks of treatment (FIG. 5 ).

After 2 months of disease progression following the ⅚ nephrectomysurgery (Day 0), ⅚ nephrectomized mice (CKD) exhibited a significantdecrease in BMD compared to the sham cohorts (-5.4%, P<0.01). Through 6weeks of treatment the mActRIIA-Fc treated sham and CKD cohorts hadsignificantly greater BMD compared to their VEH treated counterparts(FIG. 6 ).

At the conclusion of the study the hind limbs were collected and fixedin 70% ethanol. The right femur was microCT scanned (VivaCT 75, Scanco)to quantify cortical and trabecular bone structure. FIG. 7 showscross-sectional images of femurs from each treatment group.Nephrectomized mice exhibited decreased cortical thickness and noobvious changes to trabecular bone structure.

mActRIIA-Fc treated mice exhibited increases in both cortical thicknessand trabecular bone volume. Analyses of the femur mid-shaft were used toquantify the mean cortical thickness in each cohort (FIG. 8 ). The CKDmice had thinner cortical bones than their sham counterparts in both theVEH (P<0.01) and mActRIIA-Fc (P<0.01) cohorts. mActRIIA-Fc treated micehad a significant increase in cortical thickness in both the sham (+17%,P<0.01) and CKD (+19.2%, P<0.01) cohorts compared to their respectiveVEH-treated mice. As evidenced by the sample images in FIG. 7 , analysesof the distal femur revealed dramatic increases in trabecular bonevolume and thickness in mActRIIA-Fc treated mice. mActRIIA-Fc was ableto significantly increase trabecular bone volume (FIG. 9 ) andtrabecular thickness (FIG. 10 ) over VEH treated mice in both the shamand CKD cohorts. Measurements of trabecular bone volume demonstrated atweek 8 that mActRIIA-Fc treated mice had a significant increase intrabecular bone volume in both the sham (+549%, P<0.001) and CKD (+827%,P<0.001) cohorts compared to their respective VEH-treated mice.Measurements of trabecular thickness demonstrated at week 8 thatmActRIIA-Fc treated mice had a significant increase in trabecularthickness in the CKD (+62%, P<0.001) cohorts compared to theirrespective VEH-treated mice.

At terminal sacrifice whole blood was taken from all animals andprocessed for serum. Serum samples were analyzed using a Vetscan VS2analyzer (Abaxis, Inc) using a comprehensive profile rotor. Mean valuesfor the analyates from each group are shown in Table 3. Comparison ofthe SHAM and CKD vehicle control groups showed increases in blood ureanitrogen (BUN) and creatinine (CRE) as expected due to impaired renalfunction. Additionally the ALT and amylase (AMY) were increased in CKDmice due to altered kidney function or suggestive of the nephrectomyalso altering liver function. Calcium (CA++) and total alkalinephosphates (ALP) levels also increased as expected due to increased boneturnover. mActRIIA-Fc treatment increased ALP levels in both the SHAMand CKD mice due to the bone anabolic properties of the drug. In CKDmice mActRIIA-Fc treatment decreased albumin (ALB), total protein (TP)and CRE levels compared to CKD-VEH controls, but were not different thanSHAM mice. These changes are not thought to be relevant to the model orthe treatment at this point.

TABLE 3 SHAM VEH SHAM mActRIIA-Fc CKD VEH CKD mActRIIA-Fc AMY U/L 865.45± 39.41 803.38 ± 66.06 1486.18 ± 53.82 1418.42 ± 36.68 TBIL mg/dL 0.25 ±0.02 0.23 ± 0.02 0.23 ± 0.01 0.27 ± 0.01_(u̅) BUN mg/dL 27.92 ± 1.3929.20 ± 1.26 52.75 ± 2.66 51.50 ± 2.10 CA₊₊ mg/dL 10.18 ± 0.16 10.38 ±0.12 11.00 ± 0.13 11.33 ± 0.13 PHOS mg/dL 8.58 ± 0.17 8.96 ± 0.28 8.28 ±0.36 7.96 ± 0.26 CRE mg/dL 0.33 ± 0.05 0.40 ± 0.05 0.44 ± 0.05 0.31 ±0.02_(u̅) GLU mg/dL 198.50 ± 6.52 260.90 ± 28.79* 223.67 ± 13.53 260.86 ±14.98 NA₊ mmol/L 156.50 ± 0.77 157.60 ± 0.73 158.58 ± 2.37 155.64 ± 0.34K₊ mmol/L 7.65 ± 0.14 7.85 ± 0.15 7.98 ± 0.14 7.77 ± 0.13 TP g/dL 5.66 ±0.05 5.42 ± 0.057 * 5.73 ± 0.08 5.47 ± 0.07_(ū) GLOB g/dL 1.79 ± 0.081.67 ± 0.06 1.73 ± 0.07 1.97 ± 0.06_(ū) *= p<0.05 vs SHAM VEH; ++ =p<0.05 vs CKD VEH

(C) Conclusions

Treatment with mActRIIA-Fc was able to prevent anemia and bone loss in a⅚ nephrectomy model of chronic kidney disease. CKD mice were anemic, hadlower BMD and thinner cortical bone structure in the femur when comparedto the sham counterparts. mActRIIA-Fc treatment of CKD mice increasedthe hematocrit, BMD and cortical bone structure significantly over theVEH treated mice. Furthermore, mActRIIA-Fc was able to increasetrabecular bone volume and trabecular thickness in the CKD mice tovalues greater than the VEH treated mice in both the sham and CKDcohorts. These data demonstrate that blocking Activin receptor IIAsignaling by mActRIIA-Fc administration can prevent anemia and bone lossin the ⅚ nephrectomy model of chronic kidney disease.

6.4 Prophetic Example-mActRIIA Inhibition to Treat Adynamic Bone Diseasein CDK Context

Mice are subj ected to electrocautery of one kidney and nephrectomy ofthe other kidney. The mice are fed low-phosphate chow supplemented withcalcitriol. See, e.g., Lund et al., 2004, J Am Soc Nephrol 15:349-369.

This study is designed to study the effects of soluble mouse ActRIIAthat is fused with mouse Fc via a minimal linker (SEQ ID NO:15) ontreatment of blood and bone parameters in a mouse model of adynamic bonedisorder.

Mice with electrocautery of one kidney and nephrectomy of the otherkidney are used as a model of adynamic bone in CKD (“ADB”) context totest the effects of the polypeptide with the amino acid sequence of SEQID NO:15 in this model. Mice receive two surgeries to 1) remove onekidney completely and 2) electrocautery of the other kidney. Shamoperated mice are also included as controls. The surgeries can beconducted as described in Lund et al., 2004, J Am Soc Nephrol15:349-369.

One group of mice is placed on low-phosphate chow supplemented withcalcitriol diet. Another group of mice is placed on normal chow dict.Two weeks after the final surgery mice are divided into groups (bothSHAM and ADB) and administration begins with vehicle (PBS) ormActRIIA-Fc at 10 mg/kg twice per week for 8 weeks. Complete bloodcounts (CBC) are taken periodically during the study to assess foranemia.

Bone mineral density is determined using dual energy x-rayabsorptiometry (DEXA, PIXIMus). At the conclusion of the studynecropsies are conducted to collect the long bones of the hind limbs andmajor organs. The remnant kidney is sent for histology processing andstaining with H&E or Trichrome stain. Femurs are scanned by uCT (Scanco)to determine bone microarchitecture. Quantitative computed tomography(QCT) can also be used to determine bone turnover.

6.5 Effects of ActRIIA Inhibition on Vascular Calcification

This Example demonstrates that inhibiting ACTRIIA is effective inreducing calcium levels in the vasculature of subjects, and thusrepresents a means for treating vascular calcification.

Stage 3 chronic kidney disease (CKD) was induced in 14-week oldldlr^(-/-) mice (C57B1/6J background; Jackson Laboratory) that were fedhigh fat diets (“CKD micc”). Low-density lipoprotein receptor (ldlr) isknown to be involved in lipid clearance, and ldlr knockout micerepresent a model of atherosclerosis. The ldlr deficient mice that arefed high fat/cholesterol diets develop atherosclerosis, and aorticplaque associated calcification that is stimulated by CKD induced byrenal ablation. CKD was induced in the ldlr^(-/-) mice by ⅚ nephrectomy(see above). As described above, the ⅚ nephrectomy comprises completeremoval of one kidney followed by ligation of 2 of the 3 renal veins inthe remaining kidney.

By week 22, vascular calcification is established in the CKD mice, asconfirmed by chemical calcification quantitation. Briefly, hearts andaorta from the mice are dissected at sacrifice, and all extraneoustissue is removed by blunt dissection under a dissecting microscope.Tissues are desiccated for 20-24 hours at 55° C., weighed and crushed toa powder with a pestle and mortar. Calcium is eluted in 10% formic acid(10:1 v/w) for 24 hours at 4° C. Calcium content of eluate is assayedusing a cresolphthalein complexone method (Sigma, St Louis), accordingto manufacturers instructions, and results are corrected for dry tissueweight.

The CKD mice were divided into two experimental groups (i) mActRIIA-Fctreated mice; and (ii) CKD-3-Vchiclc mice, which were administered thevehicle portion only of the mActRIIA-Fc composition (i.e., the mice wereadministered a saline composition without mActRIIA-Fc).mActRIIA-Fc-treated mice (n=5) were administered 10 mg/kg of mActRIIA-Fctwice per week for 6 weeks. CKD-3-Vehicle mice (n=6; vehicle=saline)were administered vehicle only on the same days that mActRIIA-Fc wasadministered to the mActRIIA-Fc-treated mice. Wild-type mice (n=6;C57B1/6J background) and SHAM mice (n=8; C57B1/6J background) were usedas negative controls. SHAM mice consisted of ldlr^(-/-) mice that wereoperated on, but in which CKD was not induced (e.g., nephrectomy was notconducted). All mice were euthanized at week 28 for assessment of aorticcalcium levels in each of the four treatment groups (CKD-3-Vehicle;mActRIIA-Fc-treated; SHAM; and wild-type).

Table 4, below, provides the aortic calcium levels observed in eachmouse used in the study (column 2), as well as the average calciumlevels for each of the SHAM, CKD-3-Vehicle, mActRIIA-Fc, and wild-typestudy groups (column 3). The results are presented in graph form in FIG.11 . As demonstrated by the data, a clear reduction in aortic calciumwas observed in the mice belonging to the mActRIIA-Fc treated groupcompared to the vehicle-treated group. In 4 of the 5 CKD mice that weretreated with mActRIIA-Fc, levels of aortic calcium were comparable tolevels observed in the two negative control groups (wild-type and SHAMmice).

Elevated vascular (e.g., arterial) calcium levels are known to beassociated with vascular calcification (see, e.g., Raggi P et al., ClinJ Am Soc Nephrol 2008; 3: 836-843). Thus, the foregoing results indicatethat ActRIIA inhibition represents a suitable approach for the treatmentand prevention of vascular calcification.

TABLE 4 Aortic Calcium Levels Experimental Group Subject Specific Ca²⁺Levels (mg/g) Average Ca²⁺ mg/g Wild-type (n = 6) 0.25, 0.11, 0.26,0.36, 0.31, 0.35 0.27 ± 0.09 Sham (n = 8) 0.28, 0.18, 0.24, 0.16, 0.13,0.25, 0.26, 0.27 0.22 ± 0.06 CKD-3-Vehicle (n = 6) 0.58, 0.17, 0.51,0.56, 0.31, 0.99 0.52 ± 0.28 mActRIIA-Fc (n = 5) 0.83, 0.28, 0.19, 0.13,0.04 0.29 ± 0.31

6.6 Effects of ActRIIA Inhibition on Vascular Calcification

This Example describes a study of the the effect of ActRII inhibition onvascular calcification in subjects with chronic kidney disease.

The mouse model of early CKD-MBD described in the preceding examples canbe used. In this model, renal ablation is added to genetic deficiency ofthe LDL receptor, ldlr, and mice are fed a high fat high cholesteroldict. In stage 3 CKD, the animals have CKD induced stimulation ofvascular calcification, decreases in bone formation, elevated FGF23levels, hyperphosphatemia, and elevated PTH levels.

(A) Materials and Methods

Animals and diets: LDL receptor null (LDLR⁻/₋) mice on a C57B1/6Jbackground or wild type C57B1/6J mice can be purchased from JacksonLaboratory (Bar Harbor, Maine) and bred in a pathogen-free environment.Animals can be weaned at three weeks to a chow diet having 6.75%calories as fat. At 10 weeks, some animals can be initiated on a highcholesterol (0.15%) diet containing 42% calories as fat (Harlan Teklad,Madison WI, Product No. TD88137), a diet that has been shown to generateatherosclerosis with vascular calcification in this genetic background(see, e.g., Towler et al., 1998, J Biol Chem 273:30427-30434). Calciumcontent in all diets can be 0.6%. Animals can be given access to waterad libitum, and maintained according to local and national animal careguidelines. mActRIIA-Fc can be administered IP (10 mg/kg) twice weekly.

Surgical Procedures: A two-step procedure can be utilized to create CKDas previously described (see, e.g., Davies et al., 2003, J Am SocNephrol 14:1559-1567; and Davies et al., 2005, J Am Soc Nephrol16:917-928). Briefly, electrocautery can be applied to the right kidneythrough a 2 cm flank incision at 10 weeks post-natal, followed by lefttotal nephrectomy through a similar incision 2 weeks later. Controlanimals can receive sham operations in which the appropriate kidney isexposed and mobilized but not treated in any other way. Intraperitonealanesthesia (xylazine 13 mg/kg and ketamine 87 mg/kg) can be used for allprocedures. Saphenous vein blood samples can be taken at 1 weekfollowing the second surgery to assess baseline post-surgical renalfunction. Animals can be sacrificed under anesthesia at 20 weeks, or 26weeks depending on the group after blood is taken by intracardiac stab.The heart and aorta can be dissected en bloc.

Tissue Preparation: Resected specimens can be fixed in formalin, andthen divided as follows: the heart, ascending aorta and aortic arch canbe separated from the descending aorta, and bisected sagittally throughthe aortic outflow tract. The descending aorta can be bisectedcoronally, approximately halfway along its length. All four pieces canbe embedded in the same wax block. Slices (5 µm thick) can be cut andstained with hematoxilin and eosin, trichrome, Alizarin Red and vonKossa.

Immunohistochemistry: Tissue sections can be prepared as above,deparaffinized in xylene, and rehydrated in graded ethanols. Endogenousperoxidase activity can be blocked by incubation in 3% hydrogen peroxide(Sigma, St Louis MO), and non-specific binding can be blocked with a10-minute incubation with a proprietary solution of casein in PBS(‘Background SNIPER’, BioCare Medical, Walnut Creek CA). Antigenretrieval can be performed with a 5-minute incubation with citratebuffer (‘Decloaker’ BioCare Medical, Walnut Creek CA) at 100° C.Sections can be incubated with affinity-purified goat polyclonalantibody against mouse osteocalcin (OC) (Biogenesis Inc, Brentwood NH)overnight, then incubated with biotinylated mouse anti-goat secondaryantibody for 10 minutes prior to streptavidin-conjugated peroxidasestaining (all reagents, BioCare Medical, Walnut Creek CA), andcounterstained with 0.1% Hematoxylin (Sigma).

RT-PCR: RNA can be extracted from tissue samples using theRNAqueous-4PCR kit (Ambion), according to the manufacturer’sinstructions. RT-PCR can be performed using the One-step RT-PCR Kit(Qiagen, Valencia CA) according to manufacturer’s instructions.Conditions can be: 50° C. for 30 min, 95° C. for 15 min, then 35-40cycles of 94° C. for 1 min, 60° C. for 1 min & 72° C. for 1 min, then72° C. for 10 min. Primer specifc to murine osteocalcin and murin GAPDHcan be selected.

Chemical Calcification Quantitation: Hearts and aorta can be dissectedat sacrifice, and all extraneous tissue removed by blunt dissectionunder a dissecting microscope. Tissues can be desiccated for 20-24 hoursat 55° C., weighed and crushed to a powder with a pestle and mortar.Calcium can be eluted in 10% formic acid (10:1 v/w) for 24 hours at 4°C. Calcium content of eluate can be assayed using a crcsolphthalcincomplexone method (Sigma, St Louis), according to manufacturersinstructions, and results can be corrected for dry tissue weight.

Bone Histomorphometry: Bone formation rate can be determined by doublefluorescence labeling. All mice can receive intraperitoneal calcein (20mg/kg) 7d and 2d before they are sacrificed. Both femurs can bedissected at the time the animals are sacrificed and placed in 70%ethanol. The specimens can be implanted undecalcified in a plasticembedding kit H7000 (Energy Beam Sciences). Bones can be sectionedlongitudinally through the frontal plane in 10-µm sections with JB-4microtome (Energy Beam Sciences). Unstained sections can be used forcalcein-labeled fluorescence analysis. Slides can be examined at X400magnification with a Leitz microscope attached to an Osteomeasure ImageAnalyzer (Osteometrics, Atlanta GA). Ten contiguous 0.0225-mm² fields ofthe distal femur, 150 µm proximal to the growth plate, can be examinedper animal.

Measurements of Parathyroid Hormone and Serum Chemistry: Blood samplescan be obtained at 2 and 8 weeks of CKD by capillary tube aspiration ofthe saphenous vein, and with a different procedure (intracardiacpuncture) at the time of sacrifice (12 weeks CKD) and transferred toheparinized tubes. After centrifugation (400X g for 5 minutes), plasmacan be removed, aliquoted and frozen at -80° C. Intact PTH levels(performed only at sacrifice because of the volume of blood required)can be measured by two-site immunoradiometric assay (IRMA) using acommercially available kit (Immutopics, San Clemente, CA). Blood ureanitrogen (BUN), serum calcium and phosphorus can be measured usingstandard multichannel analyzer techniques.

Measurements of FGF23: An FGF23 murine ELISA assay can be purchased fromthe Kainos company.

Measurements of DKKl and osteocalcin: Commercial ELISA assays for DKK1and undercarboxylated osteocalcin can be used.

Measurements of OPG and sRANKL: The ratio of OPG to RANKL can bedetermined in serum assays. These assays have been shown to correlatewell with bone turnover and excess bone resorption (see, e.g., Geusenset al., 2006, Arthritis & Rheumatism 54:1772-17775). The levels ofsRANKL in the serum can be determined by a radioimmunoassay (LincoResearch, St. Louis MO). Levels of serum OPG can be measured by an ELISAmethod (OSTEOmedical NL, Marishof, NL). The intra- and interassaycoefficients of variation (CV) are less than 10% for both tests,according to the manufacturers. The detection limit for sRANKL is 0.08pmol/l, and for OPG is 0.14 pmol/l.

Measurements of Markers of Bone Turnover: Serum P1NP and osteocalcin canbe used as markers of osteoblast activity and tartrate resistant acidphosphatase form 5b (TRACP 5b) (mouseTRAP, IDS Ltd, Bolden, UK) can beused as a marker of osteoclast levels.

Measurements of Markers of inflamation: Serum assays for TNF alpha, andc reactive protein can be used to follow the levels of inflamation andthe response to mActRIIA-Fc.

Statistical Analysis: Data can be analyzed for statistical significance(P<0.05) using ANOVA. Comparison can be made between animals treatedwith vehicle (control group) and those treated with mActRIIA-Fc.Comparison can also be made between sham-operated mice and CKD micetreated with Vehicle and mActRIIA-Fc. These analyses can be performedwith the SPSS 11.0 statistical package (Needham Heights, MA).

(B) Study Parameters

Mice used in the study can be placed into one of eight groups as shownin Table 5, below.

TABLE 5 Group Description of Group # Animals A Wild type 10 B LDLR HighFat/CKD vehicle treated euth 22 wks 10 C LDLR High Fat/CKD RAP-011treated euth 22 wks 10 D LDLR High Fat/CKD vehicle teated euth 28 wks 10E LDLR High Fat/CKD RAP-011 treated euth 28 wks 10 F LDLR High Fat/shamoperation euth 28 wks 10 G LDLR High Fat/sham operation euth 20 wks 10 HLDLR High Fat/CKD euth at 14 weeks 10

One group of animals (Group H in Table 5) can be sacrificed at 14 weeksto measure the baseline vascular calcification and histomorphometry atthe time of instituting therapy. Groups C and E can be used to assessthe efficacy of treatment with mActRIIA-Fc compared to vehicle treatedgroups (Groups B and D) over variable periods of CKD. Groups F and G areage matched sham operated high fat fed animals that can be used as thecontrol for the CKD effects. Group sizes of 10 animals per group afterrandomization into the treatment groups can be sufficient to obtainstatistical significance.

At 16-18 weeks, glomerular filtration rate (GFR) can be measured byinjection of inulin into the mice and measurment of its disappearance.At euthanasia, blood can be drawn by intracardiac stab, and serum DKK 1,FGF23, osteocalcin, PTH and calcitriol levels can be determined, alongwith serum calcium, Pi, blood urea nitrogen (BUN), glucose, andcholesterol levels.

Aortas from the ldlr-/- high fat fed CKD animals can be analyzed. Totalaortic calcium levels and von Kossa stained microscopic sections can beobtained. Aortas can be processed to obtain RNA for analysis of aorticgene expression. Aortas can be processed for immunohistochemistry. At 22weeks in the model of CKD described above, the euthanasia age for groupsB and C, vascular calcification is established and adynamic bonedisorder is present despite secondary hyperparathyroidism. Between 22and 28 weeks, vascular calcification is progressive and the effects ofthe presence of parathyroid hormone begin to increase osteoblastsurfaces.

The study described in this example can be used to determine the effectsof ActRII inhibition on vascular calcification, bone remodeling rates,and secondary hyperparathyroidism observed in subjects having CKD.

TABLE 6 Sequence Information SEQ ID NO: Description Sequence 1 humanActRIIA precursor polypeptideMGAAAKLAFAVFLISCSSGAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGPPPPSPLLGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFHEKGSLSDFLKANVVSWNELCHIAETMARGLAYLHEDIPGLKDGHKPAISHRDIKSKNVLLKNNLTACIADFGLALKFEAGKSAGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGLVLWELASRCTAADGPVDEYMLPFEEEIGQHPSLEDMQEVVVHKKKRPVLRDYWQKHAGMAMLCETIEECWDHDAEARLSAGCVGERITQMQRLTNIITTEDIVTVVTMVTNVDFPPKESSL2 human ActRIIA soluble (extracellular), processed polypeptide sequenceILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPP3 human ActRIIA soluble (extracellular), processed polypeptide sequencewith the C-terminal 15 amino acids deletedILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEM4 nucleic acid sequence encoding human ActRIIA precursor proteinATGGGAGCTGCTGCAAAGTTGGCGTTTGCCGTCTTTCTTATCTCCTGTTCTTCAGGTGCTATACTTGGTAGATCAGAAACTCAGGAGTGTCTTTTCTTTAATGCTAATTGGGAAAAAGACAGAACCAATCAAACTGGTGTTGAACCGTGTTATGGTGACAAAGATAAACGGCGGCATTGTTTTGCTACCTGGAAGAATATTTCTGGTTCCATTGAAATAGTGAAACAAGGTTGTTGGCTGGATGATATCAACTGCTATGACAGGACTGATTGTGTAGAAAAAAAAGACAGCCCTGAAGTATATTTTTGTTGCTGTGAGGGCAATATGTGTAATGAAAAGTTTTCTTATTTTCCAGAGATGGAAGTCACACAGCCCACTTCAAATCCAGTTACACCTAAGCCACCCTATTACAACATCCTGCTCTATTCCTTGGTGCCACTTATGTTAATTGCGGGGATTGTCATTTGTGCATTTTGGGTGTACAGGCATCACAAGATGGCCTACCCTCCTGTACTTGTTCCAACTCAAGACCCAGGACCACCCCCACCTTCTCCATTACTAGGGTTGAAACCACTGCAGTTATTAGAAGTGAAAGCAAGGGGAAGATTTGGTTGTGTCTGGAAAGCCCAGTTGCTTAACGAATATGTGGCTGTCAAAATATTTCCAATACAGGACAAACAGTCATGGCAAAATGAATACGAAGTCTACAGTTTGCCTGGAATGAAGCATGAGAACATATTACAGTTCATTGGTGCAGAAAAACGAGGCACCAGTGTTGATGTGGATCTTTGGCTGATCACAGCATTTCATGAAAAGGGTTCACTATCAGACTTTCTTAAGGCTAATGTGGTCTCTTGGAATGAACTGTGTCATATTGCAGAAACCATGGCTAGAGGATTGGCATATTTACATGAGGATATACCTGGCCTAAAAGATGGCCACAAACCTGCCATATCTCACAGGGACATCAAAAGTAAAAATGTGCTGTTGAAAAACAACCTGACAGCTTGCATTGCTGACTTTGGGTTGGCCTTAAAATTTGAGGCTGGCAAGTCTGCAGGCGATACCCATGGACAGGTTGGTACCCGGAGGTACATGGCTCCAGAGGTATTAGAGGGTGCTATAAACTTCGAAAGGGATGCATTTTTGAGGATAGATATGTATGCCATGGGATTAGTCCTATGGGAACTGGCTTCTCGCTGTACTGCTGCAGATGGACCTGTAGATGAATACATGTTGCCATTTGAGGAGGAAATTGGCCAGCATCCATCTCTTGAAGACATGCAGGAAGTTGTTGTGCATAAAAAAAAGAGGCCTGTTTTAAGAGATTATTGGCAGAAACATGCTGGAATGGCAATGCTCTGTGAAACCATTGAAGAATGTTGGGATCACGACGCAGAAGCCAGGTTATCAGCTGGATGTGTAGGTGAAAGAATTACCCAGATGCAGAGACTAACAAATATTATTACCACAGAGGACATTGTAACAGTGGTCACAATGGTGACAAATGTTGACTTTCCTCCCAAAGAATCTAGTCTATGA5 nucleic acid sequence encoding a human ActRIIA soluble (extracellular)polypeptideATACTTGGTAGATCAGAAACTCAGGAGTGTCTTTTCTTTAATGCTAATTGGGAAAAAGACAGAACCAATCAAACTGGTGTTGAACCGTGTTATGGTGACAAAGATAAACGGCGGCATTGTTTTGCTACCTGGAAGAATATTTCTGGTTCCATTGAAATAGTGAAACAAGGTTGTTGGCTGGATGATATCAACTGCTATGACAGGACTGATTGTGTAGAAAAAAAAGACAGCCCTGAAGTATATTTTTGTTGCTGTGAGGGCAATATGTGTAATGAAAAGTTTTCTTATTTTCCAGAGATGGAAGTCACACAGCCCACTTCAAATCCAGTTACACCTAAGCCACCC6 fusion protein comprising a soluble extracellular domain of ActRIIAfused to an Fc domain THTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVD (A)VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCK (A)VSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGPFFLYSKLTVDKSRWQQGNVFSCSVMHEALHN(A)HYTQKSLSLSPGK*7 Extracellular domain of human ActRIIA fused to a human Fc domainILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK8 Leader sequence of Honey bee mellitin (HBML) MKFLVNVALVFMVVYISYIYA 9Leader sequence of Tissue Plasminogen Activator (TPA)MDAMKRGLCCVLLLCGAVFVSP 10 Native ActRIIA leader MGAAAKLAFAVFLISCSSGA 11ActRIIA-hFc and ActRIIA-mFc N-terminal sequence ILGRSETQE 12 ActRIIA-FcProtein with deletion of the C-terminal 15 amino acids of theextracellular domain of ActRIIAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK13 Unprocessed ActRIIA-hFc with TPA leader sequenceMDAMKRGLCCVLLLCGAVFVSPGAAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK14 Nucleic acid sequence encoding Unprocessed ActRIIA-hFc with TPAleader sequenceATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCGGCGCCGCTATACTTGGTAGATCAGAAACTCAGGAGTGTCTTTTTTTAATGCTAATTGGGAAAAAGACAGAACCAATCAAACTGGTGTTGAACCGTGTTATGGTGACAAAGATAAACGGCGGCATTGTTTTGCTACCTGGAAGAATATTTCTGGTTCCATTGAATAGTGAAACAAGGTTGTTGGCTGGATGATATCAACTGCTATGACAGGACTGATTGTGTAGAAAAAAAAGACAGCCCTGAAGTATATTTCTGTTGCTGTGAGGGCAATATGTGTAATGAAAAGTTTTCTTATTTTCCGGAGATGGAAGTCACACAGCCCACTTCAAATCCAGTTACACCTAAGCCACCCACCGGTGGTGGAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGTCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGAATTC15 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the N-terminal 6 amino acids of the EC domain deleted and theC-terminal 4 amino acids of the EC domain deleted (amino acids 25-130 ofSEQ ID NO:28) and with an L79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPP16 human ActRIIB precursor protein sequence (A64) MTAPWVALALLWGSLWPGSGRGEAETRECIYY NANWELERTNQSGLER CEGEQDKRLHCYASWA NSSGTIELVKKGCWLDDFNCYDRQECVATEEN PQVYFCCCEGNFCNER FTHLPEAGGPEVTYEP PPTAPTLLTVLAYSLLPIGGLSLIVLLAFWMY RHRKPPYGHVDIHEDP GPPPPSPLVGLKPLQL LEIKARGRFGCVWKAQLMNDFVAVKIFPLQDK QSWQSEREIFSTPGMK HENLLQFIAAEKRGSN LEVELWLITAFHDKGSLTDYLKGNIITWNELC HVAETMSRGLSYLHED VPWCRGEGHKPSIAHR DFKSKNVLLKSDLTAVLADFGLAVRFEPGKPP GDTHGQVGTRRYMAPE VLEGAINFQRDAFLRI DMYAMGLVLWELVSRCKAADGPVDEYMLPFEE EIGQHPSLEELQEVVV HKKMRPTIKDHWLKHP GLAQLCVTIEECWDHDAEARLSAGCVEERVSL IRRSVNGTTSDCLVSL VTSVTNVDLPPKESSI 17 human ActRIIBsoluble (extracellular), processed polypeptide sequence (amino acids19-134 of SEQ ID NO:16)SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT18 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the C-terminal 15 amino acids deleted (amino acids 19-119 of SEQ IDNO:16)SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEA 19 nucleic acid sequence encoding a human ActRIIB (A64) precursorproteinATGACGGCGCCCTGGGTGGCCCTCGCCCTCCTCTGGGGATCGCTGTGGCCCGGCTCTGGGCGTGGGGAGGCTGAGACACGGGAGTGCATCTACTACAACGCCAACTGGGAGCTGGAGCGCACCAACCAGAGCGGCCTGGAGCGCTGCGAAGGCGAGCAGGACAAGCGGCTGCACTGCTACGCCTCCTGGGCCAACAGCTCTGGCACCATCGAGCTCGTGAAGAAGGGCTGCTGGCTAGATGACTTCAACTGCTACGATAGGCAGGAGTGTGTGGCCACTGAGGAGAACCCCCAGGTGTACTTCTGCTGCTGTGAAGGCAACTTCTGCAACGAGCGCTTCACTCATTTGCCAGAGGCTGGGGGCCCGGAAGTCACGTACGAGCCACCCCCGACAGCCCCCACCCTGCTCACGGTGCTGGCCTACTCACTGCTGCCCATCGGGGGCCTTTCCCTCATCGTCCTGCTGGCCTTTTGGATGTACCGGCATCGCAAGCCCCCCTACGGTCATGTGGACATCCATGAGGACCCTGGGCCTCCACCACCATCCCCTCTGGTGGGCCTGAAGCCACTGCAGCTGCTGGAGATCAAGGCTCGGGGGCGCTTTGGCTGTGTCTGGAAGGCCCAGCTCATGAATGACTTTGTAGCTGTCAAGATCTTCCCACTCCAGGACAAGCAGTCGTGGCAGAGTGAACGGGAGATCTTCAGCACACCTGGCATGAAGCACGAGAACCTGCTACAGTTCATTGCTGCCGAGAAGCGAGGCTCCAACCTCGAAGTAGAGCTGTGGCTCATCACGGCCTTCCATGACAAGGGCTCCCTCACGGATTACCTCAAGGGGAACATCATCACATGGAACGAACTGTGTCATGTAGCAGAGACGATGTCACGAGGCCTCTCATACCTGCATGAGGATGTGCCCTGGTGCCGTGGCGAGGGCCACAAGCCGTCTATTGCCCACAGGGACTTTAAAAGTAAGAATGTATTGCTGAAGAGCGACCTCACAGCCGTGCTGGCTGACTTTGGCTTGGCTGTTCGATTTGAGCCAGGGAAACCTCCAGGGGACACCCACGGACAGGTAGGCACGAGACGGTACATGGCTCCTGAGGTGCTCGAGGGAGCCATCAACTTCCAGAGAGATGCCTTCCTGCGCATTGACATGTATGCCATGGGGTTGGTGCTGTGGGAGCTTGTGTCTCGCTGCAAGGCTGCAGACGGACCCGTGGATGAGTACATGCTGCCCTTTGAGGAAGAGATTGGCCAGCACCCTTCGTTGGAGGAGCTGCAGGAGGTGGTGGTGCACAAGAAGATGAGGCCCACCATTAAAGATCACTGGTTGAAACACCCGGGCCTGGCCCAGCTTTGTGTGACCATCGAGGAGTGCTGGGACCATGATGCAGAGGCTCGCTTGTCCGCGGGCTGTGTGGAGGAGCGGGTGTCCCTGATTCGGAGGTCGGTCAACGGCACTACCTCGGACTGTCTCGTTTCCCTGGTGACCTCTGTCACCAATGTGGACCTGCCCCCTAAAGAGTCAAGCATCTAA20 fusion protein comprising a soluble extracellular domain of ActRIIB(A64; SEQ ID NO:17) fused to an Fc domainSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK21 fusion protein comprising a soluble extracellular domain of ActRIIB(A64) with the C-terminal 15 amino acids deleted (SEQ ID NO:18) fused toan Fc domainSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK22 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the N-terminal 6 amino acids of the EC domain deleted and theC-terminal 5 amino acids of the EC domain deleted (amino acids 25-129 ofSEQ ID NO:28) and with an L79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPP23 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the N-terminal 6 amino acids of the EC domain deleted and theC-terminal 3 amino acids of the EC domain deleted (amino acids 25-131 ofSEQ ID NO:28) and with an L79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPT24 Unprocessed ActRIIB-Fc fusion protein with the N-terminal 6 aminoacids of the EC domain deleted and the C-terminal 3 amino acids of theEC domain deleted (amino acids 25-131 of SEQ ID NO:28) and with an L79Dmutation and with TPA leader sequenceMDAMKRGLCCVLLLCGAVFVSPGAAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK* 25 Processed ActRIIB-Fc fusion protein with the N-terminal 6amino acids of the EC domain deleted and the C-terminal 3 amino acids ofthe EC domain deleted (amino acids 25-131 of SEQ ID NO:28) and with anL79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*26 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:16)GRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT27 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the C-terminal 15 amino acids deleted (amino acids 20-119 of SEQ IDNO:16)GRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEA 28 human ActRIIB precursor protein sequence (R64) MTAPWVALALLWGSLWPGSGRGEAETRECIYY NANWELERTNQSGLER CEGEQDKRLHCYASWR NSSGTIELVKKGCWLDDFNCYDRQECVATEEN PQVYFCCCEGNFCNER FTHLPEAGGPEVTYEP PPTAPTLLTVLAYSLLPIGGLSLIVLLAFWMY RHRKPPYGHVDIHEDP GPPPPSPLVGLKPLQL LEIKARGRFGCVWKAQLMNDFVAVKIFPLQDK QSWQSEREIFSTPGMK HENLLQFIAAEKRGSN LEVELWLITAFHDKGSLTDYLKGNIITWNELC HVAETMSRGLSYLHED VPWCRGEGHKPSIAHR DFKSKNVLLKSDLTAVLADFGLAVRFEPGKPP GDTHGQVGTRRYMAPE VLEGAINFQRDAFLRI DMYAMGLVLWELVSRCKAADGPVDEYMLPFEE EIGQHPSLEELQEVVV HKKMRPTIKDHWLKHP GLAQLCVTIEECWDHDAEARLSAGCVEERVSL IRRSVNGTTSDCLVSL VTSVTNVDLPPKESSI 29 human ActRIIBsoluble (extracellular), processed polypeptide sequence (amino acids19-134 of SEQ ID NO:28)SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT30 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the C-terminal 15 amino acids deleted (amino acids 19-119 of SEQ IDNO:28)SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEA 31 human ActRIIB soluble (extracellular), processed polypeptidesequence (amino acids 20-134 of SEQ ID NO:28)GRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT32 human ActRIIB soluble (extracellular), processed polypeptide sequencewith the C-terminal 15 amino acids deleted (amino acids 20-119 of SEQ IDNO:28)GRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEA 33 human ActRIIB soluble (extracellular), processed polypeptidesequence with the N-terminal 6 amino acids of the EC domain deleted andthe C-terminal 3 amino acids of the EC domain deleted (amino acids25-131 of SEQ ID NO:16) and with an L79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPT34 Unprocessed ActRIIB-Fc fusion protein with the N-terminal 6 aminoacids of the EC domain deleted and the C-terminal 3 amino acids of theEC domain deleted (amino acids 25-131 of SEQ ID NO:16) and with an L79Dmutation and with TPA leader sequenceMDAMKRGLCCVLLLCGAVFVSPGAAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK* 35 Processed ActRIIB-Fc fusion protein with the N-terminal 6amino acids of the EC domain deleted and the C-terminal 3 amino acids ofthe EC domain deleted (amino acids 25-131 of SEQ ID NO:16) and with anL79D mutationETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*36 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:28) with L79D mutationGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT37 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:16) with L79D mutationGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT38 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:28) with L79D mutation fused to an Fcdomain with a GGG linkerGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*39 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:16) with L79D mutation fused to an FcdomainGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*40 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:28) with L79D mutation fused to an Fcdomain and with TPA leader sequenceMDAMKRGLCCVLLLCGAVFVSPGASGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*41 human ActRIIB soluble (extracellular), processed polypeptide sequence(amino acids 20-134 of SEQ ID NO:16) with L79D mutation fused to an Fcdomain and with TPA leader sequenceMDAMKRGLCCVLLLCGAVFVSPGASGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWANSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*42 human ActRIIB soluble (extracellular), processed polypeptide sequencehaving a variant C-terminal sequence (disclosed in WO2007/053775)GRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEGPWASTTIPSGGPEATAAAGDQGSGALWLCLEGPAHE43 human ActRIIB soluble (extracellular), processed polypeptide sequencehaving a variant C-terminal sequence (disclosed in WO2007/053775) havingan L79D mutationGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEGPWASTTIPSGGPEATAAAGDQGSGALWLCLEGPAHE44 human ActRIIB soluble (extracellular), processed polypeptide sequencehaving a variant C-terminal sequence (disclosed in WO2007/053775) havingan L79D mutation fused to an Fc domain with a TGGG linkerGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWDDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEGPWASTTIPSGGPEATAAAGDQGSGALWLCLEGPAHETGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*45 Nucleic Acid Sequence Encoding SEQ ID NO:24 ATGGATGCAA TGAAGAGAGGGCTCTGCTGT GTGCTGCTGC TGTGTGGAGC AGTCTTCGTT TCGCCCGGCG CCGCCGAAACCCGCGAATGT ATTTATTACA ATGCTAATTG GGAACTCGAA CGGACGAACC AATCCGGGCTCGAACGGTGT GAGGGGGAAC AGGATAAACG CCTCCATTGC TATGCGTCGT GGAGGAACTCCTCCGGGACG ATTGAACTGG TCAAGAAAGG GTGCTGGGAC GACGATTTCA ATTGTTATGACCGCCAGGAA TGTGTCGCGA CCGAAGAGAA TCCGCAGGTC TATTTCTGTT GTTGCGAGGGGAATTTCTGT AATGAACGGT TTACCCACCT CCCCGAAGCC GGCGGGCCCG AGGTGACCTATGAACCCCCG CCCACCGGTG GTGGAACTCA CACATGCCCA CCGTGCCCAG CACCTGAACTCCTGGGGGGA CCGTCAGTCT TCCTCTTCCC CCCAAAACCC AAGGACACCC TCATGATCTCCCGGACCCCT GAGGTCACAT GCGTGGTGGT GGACGTGAGC CACGAAGACC CTGAGGTCAAGTTCAACTGG TACGTGGACG GCGTGGAGGT GCATAATGCC AAGACAAAGC CGCGGGAGGAGCAGTACAAC AGCACGTACC GTGTGGTCAG CGTCCTCACC GTCCTGCACC AGGACTGGCTGAATGGCAAG GAGTACAAGT GCAAGGTCTC CAACAAAGCC CTCCCAGCCC CCATCGAGAAAACCATCTCC AAAGCCAAAG GGCAGCCCCG AGAACCACAG GTGTACACCC TGCCCCCATCCCGGGAGGAG ATGACCAAGA ACCAGGTCAG CCTGACCTGC CTGGTCAAAG GCTTCTATCCCAGCGACATC GCCGTGGAGT GGGAGAGCAA TGGGCAGCCG GAGAACAACT ACAAGACCACGCCTCCCGTG CTGGACTCCG ACGGCTCCTT CTTCCTCTAT AGCAAGCTCA CCGTGGACAAGAGCAGGTGG CAGCAGGGGA ACGTCTTCTC ATGCTCCGTG ATGCATGAGG CTCTGCACAACCACTACACG CAGAAGAGCC TCTCCCTGTC CCCGGGTAAA TGA 46 fusion proteincomprising a soluble extracellular domain of ActRIIB (R64; SEQ ID NO:29)fused to an Fc domainSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPTGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK47 fusion protein comprising a soluble extracellular domain of ActRIIB(R64) with the C-terminal 15 amino acids deleted (SEQ ID NO:30) fused toan Fc domainSGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGGTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPVPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK48 full-length, unprocessed precursor protein GDF11, i.e., GDF11preproproteinMVLAAPLLLGFLLLALELRPRGEAAEGPAAAAAAAAAAAAAGVGGERSSRPAPSVAPEPDGCPVCVWRQHSRELRLESIKSQILSKLRLKEAPNISREVVKQLLPKAPPLQQILDLHDFQGDALQPEDFLEEDEYHATTETVISMAQETDPAVQTDGSPLCCHFHFSPKVMFTKVLKAQLWVYLRPVPRPATVYLQILRLKPLTGEGTAGGGGGGRRHIRIRSLKIELHSRSGHWQSIDFKQVLHSWFRQPQSNWGIEINAFDPSGTDLAVTSLGPGAEGLHPFMELRVLENTKRSRRNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS49 Nucleic acid sequence encoding SEQ ID NO: 48ATGGTGCTCGCGGCCCCGCTGCTGCTGGGCTTCCTGCTCCTCGCCCTGGAGCTGCGGCCCCGGGGGGAGGCGGCCGAGGGCCCCGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGGGGTCGGGGGGGAGCGCTCCAGCCGGCCAGCCCCGTCCGTGGCGCCCGAGCCGGACGGCTGCCCCGTGTGCGTTTGGCGGCAGCACAGCCGCGAGCTGCGCCTAGAGAGCATCAAGTCGCAGATCTTGAGCAAACTGCGGCTCAAGGAGGCGCCCAACATCAGCCGCGAGGTGGTGAAGCAGCTGCTGCCCAAGGCGCCGCCGCTGCAGCAGATCCTGGACCTACACGACTTCCAGGGCGACGCGCTGCAGCCCGAGGACTTCCTGGAGGAGGACGAGTACCACGCCACCACCGAGACCGTCATTAGCATGGCCCAGGAGACGGACCCAGCAGTACAGACAGATGGCAGCCCTCTCTGCTGCCATTTTCACTTCAGCCCCAAGGTGATGTTCACAAAGGTACTGAAGGCCCAGCTGTGGGTGTACCTACGGCCTGTACCCCGCCCAGCCACAGTCTACCTGCAGATCTTGCGACTAAAACCCCTAACTGGGGAAGGGACCGCAGGGGGAGGGGGCGGAGGCCGGCGTCACATCCGTATCCGCTCACTGAAGATTGAGCTGCACTCACGCTCAGGCCATTGGCAGAGCATCGACTTCAAGCAAGTGCTACACAGCTGGTTCCGCCAGCCACAGAGCAACTGGGGCATCGAGATCAACGCCTTTGATCCCAGTGGCACAGACCTGGCTGTCACCTCCCTGGGGCCGGGAGCCGAGGGGCTGCATCCATTCATGGAGCTTCGAGTCCTAGAGAACACAAAACGTTCCCGGCGGAACCTGGGTCTGGACTGCGACGAGCACTCAAGCGAGTCCCGCTGCTGCCGATATCCCCTCACAGTGGACTTTGAGGCTTTCGGCTGGGACTGGATCATCGCACCTAAGCGCTACAAGGCCAACTACTGCTCCGGCCAGTGCGAGTACATGTTCATGCAAAAATATCCGCATACCCATTTGGTGCAGCAGGCCAATCCAAGAGGCTCTGCTGGGCCCTGTTGTACCCCCACCAAGATGTCCCCAATCAACATGCTCTACTTCAATGACAAGCAGCAGATTATCTACGGCAAGATCCCTGGCATGGTGGTGGATCGCTGTGGCTGCTCT50 GDF11 propeptide of human GDF11 proteinAEGPAAAAAAAAAAAAAGVGGERSSRPAPSVAPEPDGCPVCVWRQHSRELRLESIKSQILSKLRLKEAPNISREVVKQLLPKAPPLQQILDLHDFQGDALQPEDFLEEDEYHATTETVISMAQETDPAVQTDGSPLCCHFHFSPKVMFTKVLKAQLWVYLRPVPRPATVYLQILRLKPLTGEGTAGGGGGGRRHIRIRSLKIELHSRSGHWQSIDFKQVLHSWFRQPQSNWGIEINAFDPSGTDLAVTSLGPGAEGLHPFMELRVLENTKRSRR51 Nucleic acid sequence encoding SEQ ID NO: 50GCCGAGGGCCCCGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGGGGTCGGGGGGGAGCGCTCCAGCCGGCCAGCCCCGTCCGTGGCGCCCGAGCCGGACGGCTGCCCCGTGTGCGTTTGGCGGCAGCACAGCCGCGAGCTGCGCCTAGAGAGCATCAAGTCGCAGATCTTGAGCAAACTGCGGCTCAAGGAGGCGCCCAACATCAGCCGCGAGGTGGTGAAGCAGCTGCTGCCCAAGGCGCCGCCGCTGCAGCAGATCCTGGACCTACACGACTTCCAGGGCGACGCGCTGCAGCCCGAGGACTTCCTGGAGGAGGACGAGTACCACGCCACCACCGAGACCGTCATTAGCATGGCCCAGGAGACGGACCCAGCAGTACAGACAGATGGCAGCCCTCTCTGCTGCCATTTTCACTTCAGCCCCAAGGTGATGTTCACAAAGGTACTGAAGGCCCAGCTGTGGGTGTACCTACGGCCTGTACCCCGCCCAGCCACAGTCTACCTGCAGATCTTGCGACTAAAACCCCTAACTGGGGAAGGGACCGCAGGGGGAGGGGGCGGAGGCCGGCGTCACATCCGTATCCGCTCACTGAAGATTGAGCTGCACTCACGCTCAGGCCATTGGCAGAGCATCGACTTCAAGCAAGTGCTACACAGCTGGTTCCGCCAGCCACAGAGCAACTGGGGCATCGAGATCAACGCCTTTGATCCCAGTGGCACAGACCTGGCTGTCACCTCCCTGGGGCCGGGAGCCGAGGGGCTGCATCCATTCATGGAGCTTCGAGTCCTAGAGAACACAAAACGTTCCCGGCGG52 Mature human GDF11 proteinNLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS53 Nucleic acid sequence encoding SEQ ID NO: 52AACCTGGGTCTGGACTGCGACGAGCACTCAAGCGAGTCCCGCTGCTGCCGATATCCCCTCACAGTGGACTTTGAGGCTTTCGGCTGGGACTGGATCATCGCACCTAAGCGCTACAAGGCCAACTACTGCTCCGGCCAGTGCGAGTACATGTTCATGCAAAAATATCCGCATACCCATTTGGTGCAGCAGGCCAATCCAAGAGGCTCTGCTGGGCCCTGTTGTACCCCCACCAAGATGTCCCCAATCAACATGCTCTACTTCAATGACAAGCAGCAGATTATCTACGGCAAGATCCCTGGCATGGTGGTGGATCGCTGTGGCTGCTCT 54 Extracellular domain ofmurine ActRIIA fused to a murine Fc domain (“mActRIIA-Fc”) Murinecounterpart of SEQ ID NO: 7. Comprises murine IgG2a fused to theextracellular domain of AcrRIIA.

EQUIVALENTS

Although the invention is described in detail with reference to specificembodiments thereof, it will be understood that variations which arefunctionally equivalent are within the scope of this invention. Indeed,various modifications of the invention in addition to those shown anddescribed herein will become apparent to those skilled in the art fromthe foregoing description and accompanying drawings. Such modificationsare intended to fall within the scope of the appended claims. Thoseskilled in the art will recognize, or be able to ascertain using no morethan routine experimentation, many equivalents to the specificembodiments of the invention described herein. Such equivalents areintended to be encompassed by the following claims.

All publications, patents and patent applications mentioned in thisspecification are herein incorporated by reference into thespecification to the same extent as if each individual publication,patent or patent application was specifically and individually indicatedto be incorporated herein by reference in their entireties.

What is claimed:
 1. A method for treating an adynamic bone disorder in asubject, wherein the method comprises administering a therapeuticallyeffective amount of an ActRII inhibitor to a subject in need oftreatment of the adynamic bone disorder.
 2. A method for treating anadynamic bone disorder form of CKD-MBD in a subject, wherein the methodcomprises administering a therapeutically effective amount of an ActRIIinhibitor to a subject in need of treatment of the adynamic bonedisorder form of CKD-MBD.
 3. The method of claim 1 or 2, wherein theadynamic bone disorder is characterized by absence of tetracyclineincorporation into mineralized bone.
 4. A method for treating a low boneturnover form of CKD-MBD in a subject, wherein the method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto a subject in need of treatment of the low bone turnover form ofCKD-MBD.
 5. The method of claim 4, wherein the low bone turnover form ofCKD-MBD is osteomalacia.
 6. A method for treating a bone disordercharacterized by hyperphosphatemia in a subject, wherein the methodcomprises administering a therapeutically effective amount of an ActRIIinhibitor to a subject in need of treatment of the bone disordercharacterized by hyperphosphatemia.
 7. A method for treating vascularcalcification in a subject, wherein the method comprises administering atherapeutically effective amount of an ActRII inhibitor to a subject inneed of treatment of atherosclerotic calcification.
 8. A method fortreating a renal disease in a subject, wherein the method comprisesadministering a therapeutically effective amount of an ActRII inhibitorto a subject in need of treatment of the renal disease.
 9. The method ofclaim 8, wherein the renal disease is renal fibrosis.
 10. A method forreducing vascular calcium levels in a subject diagnosed with vascularcalcification, wherein the method comprises administering atherapeutically effective amount of an ActRII inhibitor to the subject.11. The method of claim 1, 2, 4, 6, 7, 8, or 10 wherein the ActRIIinhibitor is a polypeptide comprising an amino acid sequence selectedfrom the group consisting of: a. 90% identical to SEQ ID NO:2; b. 95%identical to SEQ ID NO:2; c. 98% identical to SEQ ID NO:2; d. SEQ IDNO:2; e. 90% identical to SEQ ID NO:3; f. 95% identical to SEQ ID NO:3;g. 98% identical to SEQ ID NO:3; h. SEQ ID NO:3; i. 90% identical to SEQID NO:6; j. 95% identical to SEQ ID NO:6; k. 98% identical to SEQ IDNO:6; l. SEQ ID NO:6; m. 90% identical to SEQ ID NO:7; n. 95% identicalto SEQ ID NO:7; o. 98% identical to SEQ ID NO:7; p. SEQ ID NO:7; q. 90%identical to SEQ ID NO: 12; r. 95% identical to SEQ ID NO: 12; s. 98%identical to SEQ ID NO: 12; t. SEQ ID NO:12; u. 90% identical to SEQ IDNO: 17; v. 95% identical to SEQ ID NO: 17; w. 98% identical to SEQ IDNO: 17; x. SEQ ID NO: 17; y. 90% identical to SEQ ID NO:20; z. 95%identical to SEQ ID NO:20; aa. 98% identical to SEQ ID NO:20; bb. SEQ IDNO:20; cc. 90% identical to SEQ ID NO:21; dd. 95% identical to SEQ IDNO:21; ee. 98% identical to SEQ ID NO:21; ff. SEQ ID NO:21 gg. 90%identical to SEQ ID NO:23; hh. 95% identical to SEQ ID NO:23; ii. 98%identical to SEQ ID NO:23; jj. SEQ ID NO:23 kk. 90% identical to SEQ IDNO:25; ll. 95% identical to SEQ ID NO:25; mm. 98% identical to SEQ IDNO:25; and nn. SEQ ID NO:25.
 12. The method of claim 1, 2, 4, 6, 7, 8,or 10, wherein the ActRII inhibitor is a polypeptide comprising theamino acid sequence of SEQ ID NO:7.
 13. The method of claim 1, 2, 4, 6,7, 8, or 10, wherein the ActRII inhibitor is administered parentally.14. The method of claim 1, 2, 4, 6, 7, 8, or 10, wherein the subject isless than 18 years old.
 15. The method of claim 1, 2, 4, 6, 7, 8, or 10,wherein the method increases the height of the subject.
 16. The methodof claim 1, 2, 4, 6, 7, 8, or 10, wherein the subject has end stagerenal disease.
 17. The method of claim 1, 2, 4, 6, 7, 8, or 10, whereinthe subject undergoes dialysis.